数字农科院2.0

The Yr9 gene encoding a CC-NBS-LRR protein in the 1RS-1BL translocation confers wheat stripe rust resistance

文献类型： 外文期刊

作者： 王春挥;常亚南;王冕;王婧;刘畅;范超兰;易从扬;周晨;袁静;杨武云;刘登才;王涛;刘阳;叶兴国;韩方普

关键词： Yr9; Yr9-b; stripe rust; rye; NLR; 1RS·1BL.

期刊名称： Science China-Life Sciences

ISSN： 1674-7305

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Stripe rust, a globally widespread disease, stands as one of the most significant threats to wheat cultivation. The 1RS·1BL translocation, renowned for its robust resistance to both rust and powdery mildew, has historically played an important role in wheat breeding and production. The gene for resistance to stripe rust on the 1RS is known as Yr9 and plays an important role in the production of wheat, but over the course of long-term breeding had lost its resistance due to the evolution of stripe rust towards greater and greater virulence. In this study, we cloned the stripe rust resistance gene Yr9-b in triticale via genetic mapping and Yr9 from the 1RS·1BL translocation through homologous cloning. The Yr9 and Yr9-b encodes a typical nucleotide-binding leucine-rich repeat (NLR) protein. Through overexpression, transgenic driven by its own promoter, and gene knockout, we demonstrated the stripe rust resistance function of Yr9-b. The transgenic wheat plants expressing Yr9-b controlled by its native promoter exhibited strong resistance to stripe rust without any visible pustules after inoculated with the CYR17 race. Additionally, despite the four amino acid differences between the Yr9 and Yr9-b, both exhibited identical resistance to stripe rust. Both the Yr9 and Yr9-b loses resistance when deployed in the field or inoculated with mixed physiological races collected from the field. Our findings provide valuable insights for breeders to strategically incorporate disease resistance genes and provides a foundation for further understanding how pathogenic fungus might evolve to evade recognition via NLR type proteins.

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