数字农科院2.0

The DNA N6-adenine methylation target gene GhMAF1 promotes fiber initiation at the base of Gossypium ovules

文献类型： 外文期刊

作者： Pei, Yanfei;Liu, Yongming;Peng, Jun;Pei, Yayue;Zhang, Tianen;Miao, Pengfei;Liu, Yuanyuan;Liu, Yang;Liu, Ji;Yang, Zuoren;Li, Fuguang;Wang, Zhi

作者机构：

关键词： 6mA methylation;cotton;fiber initiation;GhMAF1;spatial differentiation

期刊名称： NEW PHYTOLOGIST

ISSN： 0028-646X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： DNA methylation consists of 5-methylcytosine and N6-methyl deoxyadenosine (6mA) and is crucial in plant development. However, its specific role and potential mechanism to initiate cotton fibers remain unclear. This study employed Oxford Nanopore Technologies (ONT) sequencing to analyze DNA methylation alterations in ZM24 and ZM24 fuzzless-lintless (ZM24fl) during fiber initiation. Our results indicated that DNA 6mA methylation exhibited the most remarkable difference among ovule samples at -2, 0, and 5 d post anthesis of ZM24 and ZM24fl. Subsequently, genes with significant changes in DNA 6mA methylation and transcription during fiber initiation were screened. We found that GhMAF1 displayed significant transcriptional upregulation and 6mA enrichment in its promoter, which could serve as a potential target for DNA 6mA in fiber initiation. Further, we knocked out GhMAF1 using CRISPR-Cas technology and demonstrated that GhMAF1 specifically promotes the initiation of fiber cells at the base of the ovule by mediating the downstream JAZ2/CPC-MML3/MML4 pathway. These findings unveil a novel spatial module of fiber cell initiation on the ovule surface that involves GhMAF1. Ultimately, this work provides significant knowledge for the regulatory network of DNA 6mA modification in fiber initiation to improve fiber yield and quality.

分类号：

  • 相关文献

[1]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[2]2015年全国棉花种植品种监测报告——播种品种(系)344个;数量减少83个;减幅19.4%. 毛树春,冯璐,芦建华. 2016

[3]2015年中国与美国棉花种植品种比较. 冯璐,毛树春. 2016

[4]兰州百合病毒病原的DAS-ELISA检测. 王发林,古勤生,刘芬,彭斌,刘丽锋. 2003

[5]The DNA N6-adenine methylation target gene GhMAF1 promotes fiber initiation at the base of Gossypium ovules. . 2025

[6]Transcriptomic analysis reveals the key role of histone deacetylation via mediating different phytohormone signalings in fiber initiation of cotton. Zhenzhen Wei,Yonghui Li,Faiza Ali,Ye Wang,Jisheng Liu,Zuoren Yang,Zhi Wang,Yadi Xing,Fuguang Li. 2022

[7]RNA-Seq with a novel glabrous-ZM24fl reveals some key lncRNAs and the associated targets in fiber initiation of cotton. Xianyan Zou,Faiza Ali,Shuangxia Jin,Fuguang Li,Zhi Wang. 2022

[8]A Retrotransposon Insertion in GhMML3_D12 Is Likely Responsible for the Lintless Locus li3 of Tetraploid Cotton. . 2020

[9]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[10]Genome-Scale Analysis Of The Wri-Like F.amily In Gossypium And F unctional Characterization Of Ghwri1A Controlling Triacylglycerol Content. Wang, Nuohan,Ma, Jianjiang,Liu, Guoyuan,Yu, Jiwen,Geng, Yanhui,Wu, Man,Li, Dan,Zhang, Jinfa,Zang, Xinshan,Pei, Wenfeng,Cui, Yupeng,Li, Xingli. 2018

[11]Genome-Wide Identification And Expression Analysis O.f The Metacaspase Gene F amily In Gossypium Species. Liu, Aiying,Zhang, Zhen,Jiang, Xiao,Zhang, Zhibin,Fan, Senmiao,Deng, Xiaoying,Ge, Qun,Lei, Kang,Gong, Juwu,Li, Junwen,Shi, Yuzhen,Yuan, Youlu,Huang, Jinyong,Gong, Wankui,Zou, Xianyan,Jia, Tingting,Zhang, Lipeng,Yuan, Youlu,Shang, Haihong,Fan, Liqiang,Zhang, Shuya,Shang, Haihong. 2019

[12]Gbs Mapping And Analysis Of G.enes Conserved Between Gossypium T omentosum And Gossypium Hirsutum Cotton Cultivars That Respond To Drought Stress At The Seedling Stage Of The Bc2F2 Generation. Hou, Yuqing,Magwanga, Richard Odongo,Lu, Pu,Kirungu, Joy Nyangasi,Magwanga, Richard Odongo,Xu, Yanchao,Hu, Yangguang,Zhou, Zhongli,Diouf, Latyr,Liu, Fang,Dong, Qi,Wang, Xingxing,Cai, Xiaoyan,Wang, Kunbo. 2018

[13]Molecular Characterisation And Functional Analysis O.f A Cytochrome P450 G ene In Cotton. Sun, Quan,Wang, Weina,Shi, Yuzhen,Sun, Quan,Wang, Weina,Zhang, Xiao,Zhou, Kexue,Yuan, Youlu,Zhang, Xiangrui,Mo, Jianchuan,Cheng, Jieru,Chu, Zongyan,Cai, Chaowei,Cai, Yingfan,Du, Xiongming,Long, Lu,Miao, Chen,Gao, Wei,Liu, Yujia. 2017

[14]Molecular Evolution And Stress And P.hytohormone Responsiveness Of Sut G enes In Gossypium Hirsutum. Liu, Yangai,Ma, Xiongfeng,Pei, Xiaoyu,Wang, Zhenyu,Song, Chengxiang,Sun, Kuan,Yang, Daigang,Zhou, Xiaojian,Zhang, Fei,Ren, Zhongying,He, Kunlun,Li, Wei. 2018

[15]Topical Shading Substantially Inhibits Vegetative B.ranching By Altering Leaf P hotosynthesis And Hormone Contents Of Cotton Plants. Li, Cundong,Dong, Hezhong,Kong, Xiangqiang,Kong, Xiangqiang,Dai, Jianlong,Dong, Hezhong,Dong, Hezhong,Li, Ting,Zhang, Yanjun,Li, Ting. 2019

[16]Rna-Sequencing, Physiological And Rnai Analyses P.rovide Insights Into The R esponse Mechanism Of The Abc-Mediated Resistance To Verticillium Dahliae Infection In Cotton. Peng, Renhai,Magwanga, Richard Odongo,Lu, Pu,Wang, Kunbo,Wang, Xingfen,Zhou, Zhongli,Xu, Yanchao,Wang, Xingxing,Cai, Xiaoyan,Dong, Qi,Dong, Qi,Hou, Yuqing,Magwanga, Richard Odongo,Liu, Fang,Kirungu, Joy Nyangasi,Ma, Zhiying. 2019

[17]Cloning, Sequence and Expression Analysis of Gossypium barbadense L.pepc Gene. Yu Jiwen,Yu Shuxun,Zhai Honghong,Fan Shuli,Song Meizhen,Zhang Jinfa. 2011

[18]Identification And Functional Analysis Of T.wo Cotton Orthologs Of M ax2 Which Control Shoot Lateral Branching. Zhao, Jinfeng,Fang, Jingjing,Zhao, Linlin,Li, Xueyong,Xing, Jin,Peng, Peng,Zhang, Wenhui,Long, Haixin,Zhao, Linlin,Liu, Weina. 2017

[19]Transcriptome Analysis And Identification Of G.enes Associated With Fruiting B ranch Internode Elongation In Upland Cotton. Shen, Qian,Zhang, Yongjiang,Pang, Chaoyou,Chen, Jing,Ju, Feiyan,Zhao, Xinhua,Liu, Shaodong,Ju, Feiyan,Zhang, Xiaomeng,Ma, Huijuan,Ge, Changwei,Zhang, Siping. 2019

[20]Dry Matter Accumulation And Phosphorus E.fficiency Response Of Cotton C ultivars To Phosphorus And Drought. Li Yongshen,Guan Xinyuan,Liu Ping,Liu Zhiyong,Wu Zhansheng,Wang Jun. 2017

作者其他论文 更多>>