数字农科院2.0

Multi-chromatic and multi-component lateral flow immunoassay for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified crops

文献类型： 外文期刊

作者： Yang, Yao;Zhang, Zini;Wang, Zhi;Pan, Ruxin;Wu, Huimin;Zhai, Shanshan;Wu, Gang;Fu, Wei;Gao, Hongfei

作者机构：

关键词： Multi-component analysis;Lateral flow immunoassay;Visual detection;Genetically modified crop;Polystyrene microspheres;On-site detection

期刊名称： MICROCHIMICA ACTA

ISSN： 0026-3672

年卷期： 2025 年 192 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： A multi-chromatic and multi-component lateral flow immunoassay (MCMC-LFIA) was developed for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified (GM) crops. Captured antibodies specific to these exogenous proteins were separately immobilized on a nitrocellulose membrane as test zones. Multi-colored microspheres, used as visible multi-probes, were conjugated with corresponding antibodies and sprayed on the conjugate pad. The assay results can be visually interpreted within 10 min by observing the appearance of colored bands. The MCMC-LFIA demonstrated high sensitivity, with detection of limits of 7.8 ng/mL for CP4 EPSPS and 2.5 ng/mL for Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins, significantly improving the performance of previously reported LFIAs. The MCMC-LFIA exhibited excellent specificity and was validated for practical use in field-based applications. The proposed MCMC-LFIA offers a rapid, sensitive, and user-friendly tool for the on-site large-scale screening of GM materials.

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