文献类型： 外文期刊
作者： Lv, Ping;Su, Fei;Chen, Fangyuan;Yan, Chunxue;Xia, Dandan;Sun, Hui;Li, Shanshan;Duan, Zhiqiang;Ma, Changle;Zhang, Hui;Wang, Mugui;Niu, Xiaomu;Zhu, Jian-Kang;Zhang, Jinshan
作者机构：
关键词： CRISPR/Cas12i3;Multiplex DR-spacer Array;DNA structural variations;Base editors
期刊名称： PLANT BIOTECHNOLOGY JOURNAL
ISSN： 1467-7644
年卷期： 2023 年
页码：
收录情况： SCIE（2023版）
摘要： The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.
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