数字农科院2.0

MiR408 balances plant growth and heat response in rice

文献类型： 外文期刊

作者： Fengting Wen;Yu Gao;Yuxiang Zeng;Guangyan Li;Baohua Feng;Hubo Li;Tingting Chen;Danying Wang;Longxing Tao;Jie Xiong;Guanfu Fu

作者机构：

关键词： Energy homeostasis;Heat response;MiR408;Plant growth;Rice (Oryza sativa L.)

期刊名称： Environmental and Experimental Botany

ISSN： 0098-8472

年卷期： 2024 年 221 卷

页码：

收录情况： SCIE（2024版）

摘要： MicroRNAs are small noncoding RNAs that regulate gene expression by targeting complementary mRNA molecules. Among them, microRNA408 (miR408) is an ancient microRNA influencing plant growth and aiding in abiotic stress adaptation. We proved that miR408 sliced uclacyanin7 (UCL7) directly by degradome sequencing. However, the mechanisms underlying the balance between growth and stress response of rice (Oryza sativa L.) controlled by miR408 remain unclear. We conducted a study comparing Nipponbare (wild-type, WT) plants with miR408–3p-overexpression (miR408–3p-OX) and UCL7-overexpression (UCL7-OX) plants under normal and heat stress conditions. Through RNA-sequencing, physiological measurements, and phenotype examination, we observed that miR408 overexpression resulted in increased dry weight and grain yield, higher levels of ATP and non-structural carbohydrates (NSC), improved photosynthesis, and reduced H2O2 content, antioxidant enzyme activity, and ATPase activity in seedling plants compared to UCL7-OX plants. Additionally, the application of H2O2 scavengers, such as ascorbic acid (ASA) and dimethylthiourea (DMTU), further enhanced dry weight and photosynthesis by reducing H2O2 levels. Under heat stress conditions, miR408 overexpression sharply increased H2O2 content, antioxidant enzyme activity, ATP levels, and ATPase activity compared to UCL7-OX plants, thereby enhancing heat tolerance in rice seedlings. These findings suggest that miR408 and its target gene UCL7 participate in regulating energy homeostasis to achieve a balance between growth and heat response. This regulation involves modulating H2O2 levels and optimizing the relationship between the target of rapamycin (TOR) and SNF1-related protein kinase 1 (SnRK1) in rice.

分类号：

  • 相关文献

[1]Involvement of 1-Methylcyclopropene in Plant Growth, Ethylene Production, and Synthase Activity of Inferior Spikelets in Hybrid Rice Differing in Panicle Architectures. Zhang, Junhua,Zhu, Lianfeng,Yu, Shengmiao,Jin, Qianyu,Zhang, Junhua.

[2]Recent Advances in Transcriptome Analysis Within the Realm of Low Arsenic Rice Breeding. Guanrong Huang,Guoping Yu,Huijuan Li,Haipeng Yu,Zengying Huang,Lu Tang,Pengfei Yang,Zhengzheng Zhong,Guocheng Hu,Peng Zhang,Hanhua Tong. 2025

[3]Genetic Dissection for Leaf Correlative Traits of Rice (Oryza sativa L.)Under Drought Stress. Guo Longbiao,Qian Qian,Zeng Dali,Dong Guojun,Teng Sheng,Zhu Lihuang. 2004

[4]Genes and pathways correlated with heat stress responses and heat tolerance in maize kernels. Yan Chen,Tingting Du,Jie Zhang,Shoukun Chen,Junjie Fu,Huihui Li,Qin Yang. 2023

[5]The Evolution and Functional Roles of miR408 and Its Targets in Plants. Yu Gao,Baohua Feng,Caixia Gao,Huiquan Zhang,Fengting Wen,Longxing Tao,Guanfu Fu,Jie Xiong. 2022

[6]Influence of silencing the MC4R gene by lentivirus-mediated RNA interference in bovine fibroblast cells. Yang, Run-Jun,Huang, Meng,Li, Jun-Ya,Gao, Xue,Chen, Jin-Bao,Xu, Shang-Zhong,Yang, Run-Jun,Zhao, Zhi-Hui,Huang, Meng,Xu, Shang-Zhong. 2011

[7]Oligomeric Proanthocyanidins Confer Cold Tolerance in Rice through Maintaining Energy Homeostasis. Li J.,Feng B.,Yu P.,Fu W.,Wang W.,Lin J.,Qin Y.,Li H.,Chen T.,Xu C.,Tao L.,Wu Z.,Fu G.. 2023

[8]RGA1 Negatively Regulates Thermo-tolerance by Affecting Carbohydrate Metabolism and the Energy Supply in Rice. Feng, Baohua,Xu, Yongqiang,Fu, Weimeng,Li, Hubo,Li, Gengmi,Li, Juncai,Wang, Wenting,Tao, Longxing,Chen, Tingting,Fu, Guanfu. 2023

[9]Nitrogen regulates pollen tube elongation under low-light stress during anthesis to prevent spikelet abortion in rice. Yichang Zhong,Hubo Li,Feifei Li,Guanfu Fu,Qiao Deng,Dali Zeng,Linzhou Huang. 2025

[10]Phosphatidic Acid Homeostasis and Membrane Lipid Remodeling Confer Salt Tolerance in Zoysia japonica by Stabilizing Metabolic Networks and a Putative SOS Signaling Activation. Qinhao Yang,Xiangcui Zeng,Zhenzhen Liu,Zhongkuan Liu,Qiannan Hu,Mingna Li. 2025

[11]The RICE MINUTE-LIKE1 (RML1) gene, encoding a ribosomal large subunit protein L3B, regulates leaf morphology and plant architecture in rice. Zheng, Ming,Wang, Yihua,Liu, Xi,Sun, Juan,Wang, Yunlong,Xu, Yang,Lv, Jia,Long, Wuhua,Zhu, Xiaopin,Jiang, Ling,Wang, Chunming,Wan, Jianmin,Guo, Xiuping,Wan, Jianmin.

[12]Isolation and characterization of two putative cytokinin oxidase genes related to grain number per spike phenotype in wheat. Zhang, Jinpeng,Liu, Weihua,Yang, Xinming,Gao, Ainong,Li, Xiuquan,Wu, Xiaoyang,Li, Lihui.

[13]Differences of hyphal and soil phosphatase activities in drought-stressed mycorrhizal trifoliate orange (Poncirus trifoliata) seedlings. Wu, Qiang-Sheng,Zou, Ying-Ning,Wu, Qiang-Sheng,He, Xin-Hua,He, Xin-Hua,He, Xin-Hua,He, Xin-Hua.

[14]Raoultella sp strain L03 fixes N-2 in association with micropropagated sugarcane plants. Yang, Li-Tao,Li, Yang-Rui,Zhang, Ge-Min,Gao, Yi-Jing,Duan, Wei-Xing,Luo, Ting,Ou-Yang, Xue-Qing,Yang, Li-Tao,Li, Yang-Rui,Song, Xiu-Peng,Ou-Yang, Xue-Qing,An, Qianli.

[15]The alleviation of zinc toxicity by silicon is related to zinc transport and antioxidative reactions in rice. Song, Alin,Li, Ping,Li, Zhaojun,Fan, Fenliang,Liang, Yongchao,Nikolic, Miroslav.

[16]An Extended Intervarietal Microsatellite Linkage Map of Cucumber, Cucumis sativus L.. Weng, Yiqun,Staub, Jack E.,Weng, Yiqun,Johnson, Shanna,Huang, Sanwen.

[17]Effect of arbuscular mycorrhizal fungi on growth, mineral nutrition, antioxidant enzymes activity and fruit yield of tomato grown under salinity stress. Latef, Arafat Abdel Hamed Abdel,He Chaoxing.

[18]Expansins: roles in plant growth and potential applications in crop improvement. Marowa, Prince,Ding, Anming,Kong, Yingzhen.

[19]Rootstocks influence fruit oleocellosis in 'Hamlin' sweet orange (Citrus sinensis L. Osbeck). Zheng, Yongqiang,Deng, Lie,He, Shaolan,Yi, Shilai,Zheng, Yongqiang,Zhou, Zhiqin,Zhao, Xuyang,Wang, Liang.

[20]Genome-wide identification of KNOX transcription factors in cotton and the role of GhKNOX4-A and GhKNOX22-D in response to salt and drought stress. Sun, Runrun,Qin, Tengfei,Wall, Sarah Brooke,Wang, Yuanyuan,Guo, Xinlei,Sun, Jialiang,Liu, Yongsheng,Wang, Qinglian,Zhang, Baohong. 2023

作者其他论文 更多>>