数字农科院2.0

Clinically Accurate Diagnosis of Alzheimer's Disease via Single-Molecule Bioelectronic Label-Free Profiling of Multiple Blood Extracellular Vesicle Biomarkers

文献类型： 外文期刊

作者： Zheng, Jintao;Jiang, Xiaohong;Bai, Shiyao;Lai, Minchao;Yu, Jiacheng;Chen, Mingxi;Zhou, Runzhi;Jia, Yue;Yan, Haoyang;Liang, Zheng;Wang, Dian;Wu, Chuyan;Liu, Shan;Li, Chenzhong;Yang, Jinguang;Luo, Yang;Jiang, Cheng;Guo, Keying

作者机构：

关键词： alzheimer's disease;blood extracellular vesicles;label-free bioelectronic platform;multiplexed diagnostic model;organic electrochemical transistor

期刊名称： ADVANCED MATERIALS

ISSN： 0935-9648

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Alzheimer's disease (AD) is a progressive neurodegenerative disorder with no cure, making early diagnosis critical for mitigating its impact. Blood extracellular vesicles (EVs) hold promises as biomarkers for AD diagnosis, but current detection technologies lack the sensitivity and multiplexing capabilities needed for efficient diagnosis. Here, a novel label-free bioelectronic platform is presented based on an organic electrochemical transistor (OECT) integrated with a microelectrode array (MEA) for ultrasensitive detection of AD biomarkers in blood EVs, including amyloid-beta (A beta(1-40) and A beta(1-42)), total tau (t-tau), and phosphorylated tau (p-tau(181)). This platform achieves a detection limit as low as the zeptomolar (zM) level, enabling the detection of single-molecule targets. It provides a comprehensive multiplexed diagnostic model capable of delivering results within 20 min. Notably, the systematic integration of multiple AD biomarkers in blood EVs is demonstrated to significantly enhance diagnostic accuracy. This study presents a novel EVs-based multiplexed diagnostic model for AD, correctly classifying all clinical samples (n = 40), far exceeding the accuracy of a single biomarker. With its high sensitivity and rapid turnaround, this platform enables reliable AD diagnosis and holds the potential for tracking disease progression, offering a transformative tool to combat the societal burden of AD.

分类号：

  • 相关文献

[1]The effects of amyloid-beta(42) oligomer on the proliferation and activation of astrocytes in vitro. Hou, Lingling,Liu, Yanfeng,Wang, Xiaoyu,Ma, Haibin,He, Jinsheng,Zhang, Ying,Yu, Changhai,Guan, Weijun,Ma, Yuehui. 2011

[2]Screening of strain-specific Actinobacillus pleuropneumoniae genes using a combination method. Lei, Liancheng,Wang, Jiaqi,Lei, Liancheng,Du, Chongtiao,Yang, Peng,Xie, Fang,Ou, Pingyang,Han, Wenyu.

[3]Deer antler extracts reduce amyloid-beta toxicity in a Caenorhabditis elegans model of Alzheimer's disease. Fangzhou Du,Haiping Zhao,Mengjie Yao,Yanyan Yang,Jingxue Jiao,Chunyi Li. 2022

[4]Ginsenoside Rh4 Alleviates Amyloid ß Plaque and Tau Hyperphosphorylation by Regulating Neuroinflammation and the Glycogen Synthase Kinase 3ß Signaling Pathway. Ren, Zhuo,Yang, Haixia,Zhu, Chenhui,Deng, Jianjun,Fan, Daidi. 2023

[5]Protective effect of ginsenoside Rh2 on scopolamine‐induced memory deficits through regulation of cholinergic transmission, oxidative stress and the ERK‐CREB‐BDNF signaling pathway. Jingwei Lv,Cong Lu,Ning Jiang,Haixia Wang,Hong Huang,Ying Chen,Yujiao Li,Xinmin Liu. 2021

[6]Cannabidiol protects against Alzheimer's disease in C. elegans via ROS scavenging activity of its phenolic hydroxyl groups. Yue Zhang,Hongyuan Li,Sha Jin,Yuyuan Lu,Yinghua Peng,Lihui Zhao,Xiaohui Wang. 2022

[7]Self-paced semi-supervised feature selection with application to multi-modal Alzheimer's disease classification. Chao Zhang,Wentao Fan,Bo Wang,Chunlin Chen,Huaxiong Li. 2024

[8]Dietary Methionine Restriction Alleviates Cognitive Impairment in Alzheimer's Disease Mice via Sex-Dependent Modulation on Gut Microbiota and Tryptophan Metabolism: A Multiomics Analysis. Zhang, Yuyu,Liu, Tianqi,Pan, Fei,Li, Yiju,Wang, Da,Pang, Jingxi,Sang, Haojie,Xi, Yujia,Shi, Lin,Liu, Zhigang. 2025

[9]Qingke β-glucan and Lactobacillus mitigate neuroinflammation and enhance cognitive function in an Alzheimer's disease mouse model. Yusong Wu,Yong Shao,Xinyu Shao,Hailong Yu,Miao Wang,Jing Wang,Yongxin She,Jia Liu,Tangwei Zhang,Zuming Li,A. M. Abd El-Aty. 2025

作者其他论文 更多>>