数字农科院2.0

Introduction of cecropin B gene into rice (Oryza sativa L) by particle bombardment and analysis of transgenic plants

文献类型： 外文期刊

作者： Huang, DN;Zhu, B;Yang, W;Xue, R;Xiao, H;Tian, WZ;Li, LC;Dai, SH

作者机构：

关键词： cecropin B gene;particle bombardment;bar gene;rice (Oryza sativa L)

期刊名称： SCIENCE IN CHINA SERIES C-LIFE SCIENCES

ISSN： 1006-9305

年卷期： 1996 年 39 卷 6 期

页码：

收录情况： SCI

摘要： An expression vector pCB1 suitable for rice transformation, harboring a synthesized cecropin B gene and a selectable marker gene (bar), was constructed. It was introduced into immature embryos of two japonica varieties by particle bombardment, and several transgenic plants were obtained. The results from Basta treatment, PCR analysis, dot and Southern blot analysis of cecropin B gene in transgenic plants indicated that both bar and cecropin B gene were integrated into the genome of transformed plants. Northern blot analysis of transgenic plants showed the expression of cecropin B gene at transcriptional level. Some of transgenic plants revealed improved resistances to two types of bacterial diseases, rice bacterial blight and rice bacterial streak to different extent.

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