数字农科院2.0

Assessing the combinatorial cytotoxicity of the exogenous contamination with BDE-209, bisphenol A, and acrylamide via high-content analysis

文献类型： 外文期刊

作者： Jinwen Yuan;Siyan Che;Li Zhang;Xiaomin Li;Junhua Yang;Xiaoming Sun;Zheng Ruan

作者机构：

关键词： Chou-Talalay method;Combinatorial cytotoxicity;Exogenous contaminations;High-content analysis;Synergistic effects

期刊名称： Chemosphere

ISSN： 0045-6535

年卷期： 2021 年 284.0 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Food is often exposed to multiple types of contaminants, and the coexistence of contaminants may have antagonistic, additive or synergistic effects. This study investigated the combinatorial toxicity of the three most widespread exogenous contaminants, decabrominated diphenyl ether (BDE-209), bisphenol A (BPA), and acrylamide (ACR) to HepG2 cells. A mathematical model (Chou-Talalay) and high-content analysis (HCA) were used to probe the nature of the contaminants’ interactions and their cytotoxicity mechanisms, respectively. The results highlighted that for the individual pollutants, the cytotoxicity order was BDE-209> BPA > ACR, and varying combinations of contaminants exhibited additive/synergistic effects. In general, combining multiple contaminants significantly increased intracellular reactive oxygen species (ROS), Ca2+ flux, DNA damage and Caspase-3, and decreased mitochondrial membrane potential (MMP) and nucleus roundness, indicating that the additive or synergistic mechanism of the combined contaminations was disturbance to multiple organelles. This study emphasizes the complexity of human exposure to food contaminants and provides a scientific basis for formulating strict regulatory standards.

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