数字农科院2.0

Identification of the MADS-Box Gene Family and the Key Role of BrAGL27 in the Regulation of Flowering in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

文献类型： 外文期刊

作者： Xinyu Gao;Yang Li;Yun Dai;Xiangqianchen Li;Can Huang;Shifan Zhang;Fei Li;Hui Zhang;Guoliang Li;Rifei Sun;Huanzhong Song;Li Zhang;Zhendong Chen;Shujiang Zhang

作者机构：

关键词： BrAGL27;Chinese cabbage;MADS-box gene family;vernalization

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2025 年 26 卷 6 期

页码：

收录情况： SCIE（2025版）

摘要： Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a key vegetable crop in Asia, but its commercial value is often reduced by premature flowering triggered by vernalization. The molecular mechanisms behind this process are not fully understood. MADS-box genes, as crucial transcriptional regulators, play vital roles in plant development, including flowering. In this study, 102 MADS-box genes were identified in Chinese cabbage through bioinformatics analyses, covering phylogeny, chromosomal localization, and gene structure. Real-time quantitative PCR and RNA-seq data analysis revealed that the expression level of AGL27 declined as vernalization time increased. To determine BrAGL27′s functions, we obtained BrAGL27-overexpressed (OE) Arabidopsis thaliana lines that showed significantly later flowering compared with the wild type (WT). The expression levels of flowering suppressor genes AtFLC and AtTEM1 were significantly high-regulated in the BrAGL27-OE lines compared to WT plants, while the expression levels of the floral genes AtSPL15, AtSOC1, AtFT, and AtAP3 were significantly lower in the BrAGL27-overexpressed lines than in the wild type. These findings enhance understanding of MADS-box genes in vernalization and flowering regulation, offering a basis for further research on bolting resistance and flowering control in Chinese cabbage.

分类号：

  • 相关文献

[1]Exploring the Regulatory Dynamics of BrFLC-Associated lncRNA in Modulating the Flowering Response of Chinese Cabbage. Yun Dai,Xinyu Gao,Shifan Zhang,Fei Li,Hui Zhang,Guoliang Li,Rifei Sun,Shujiang Zhang,Xilin Hou. 2024

[2]BrMAF5 and Its Antisense lncRNA BrMAF5L Regulate Vernalization-Induced Bolting and Flowering in Chinese Cabbage. Li, Xiangqianchen,Gao, Xinyu,Wang, Shaoxing,Zhang, Shifan,Li, Fei,Li, Guoliang,Sun, Rifei,Zhang, Shujiang,Zhang, Hui,Dai, Yun. 2025

[3]yy Identification of vernalization responsive genes in the winter wheat cultivar Jing841 by transcriptome sequencing. Feng, Yalan,Feng, Yalan,Zhao, Yongying,Wang, Ketao,Li, Yong Chun,Wang, Xiang,Yin, Jun,Feng, Yalan,Zhao, Yongying,Li, Yong Chun,Wang, Xiang,Yin, Jun,Feng, Yalan,Zhao, Yongying,Li, Yong Chun,Wang, Xiang,Yin, Jun,Zhao, Yongying,Wang, Ketao,Wang, Ketao.

[4]The TaSOC1-TaVRN1 module integrates photoperiod and vernalization signals to regulate wheat flowering. Luo, Xumei,Liu, Bingyan,Xie, Li,Wang, Ke,Xu, Dengan,Tian, Xiuling,Xie, Lina,Li, Lingli,Ye, Xingguo,He, Zhonghu,Xia, Xianchun,Yan, Liuling,Cao, Shuanghe. 2023

[5]BnGF14-2c Positively Regulates Flowering via the Vernalization Pathway in Semi-Winter Rapeseed. Fan, Shihang,Liu, Hongfang,Liu, Jing,Hua, Wei,Li, Jun. 2022

[6]Epigenomic identification of vernalization cis-regulatory elements in winter wheat. Yanhong Liu,Pan Liu,Lifeng Gao,Yushan Li,Xueni Ren,Jizeng Jia,Lei Wang,Xu Zheng,Yiping Tong,Hongcui Pei,Zefu Lu. 2024

[7]Epigenetic insight into floral transition and seed development in plants. Mahmood T.,He S.,Abdullah M.,Sajjad M.,Jia Y.,Ahmar S.,Fu G.,Chen B.,Du X.. 2024

[8]Climate change enhances stability of wheat-flowering-date. Yong He,Wei Xiong,Pengcheng Hu,Daiqing Huang,J. Allan Feurtado,Tianyi Zhang,Chenyang Hao,Ron DePauw,Bangyou Zheng,Gerrit Hoogenboom,Laura E. Dixon,Hong Wang,Andrew Juan Challinor. 2024

[9]Time-resolved single-cell atlas identifies the spatiotemporal transcription dynamics in vernalization response in Brassica rapa. Zhicheng Zhang,Xu Cai,Jianli Liang,Jiahe Liu,Jing Guo,Wencai Yang,Xiaowu Wang,Jian Wu. 2025

[10]Comprehensive Analysis of TaNCED Gene Family in Wheat Vernalization Process. Guoqing Cui,Hao Cheng. 2025

[11]Regeneration of Chinese cabbage transgenic plants expressing antibacterial peptide gene and cowpea trypsin inhibitor gene. Zhao, Junliang,Liang, Aihua,Zhu, Zhen,Tang, Yixiong. 2006

[12]Transcriptome analysis of rosette and folding leaves in Chinese high-throughput RNA sequencing. Wang, Fengde,Li, Libin,Li, Huayin,Liu, Lifeng,Zhang, Yihui,Gao, Jianwei,Wang, Xiaowu. 2012

[13]E-beta-farnesene synergizes the influence of an insecticide to improve control of cabbage aphids in China. Dong, Jie,Liu, Ying-Jie,Liu, Yong,Heuskin, Stephanie,Lognay, Georges,Chen, Ju-Lian,Bragard, Claude,Tooker, John F.. 2012

[14]Sulfur dioxide: Relevance of toxic and nutritional effects for Chinese cabbage. Yang, Liping,Stulen, Ineke,De Kok, Luit J.. 2006

[15]Proteomic and gene expression analyses during bolting-related leaf color change in Brassica rapa. Zhang, Y. W.,Guo, M. H.,Tang, X. B.,Jin, D.,Zhang, Y. W.,Fang, Z. Y.. 2016

[16]The functional significance of E-beta-Farnesene: Does it influence the populations of aphid natural enemies in the fields?. Cui, Liang-Liang,Liu, Ying-Jie,Dong, Jie,Liu, Yong,Heuskin, Stephanie,Lognay, Georges,Chen, Ju-Lian,Song, Xu-Ming. 2012

[17]Comparative Transcriptome Analysis Reveals Heat-Responsive Genes in Chinese Cabbage (Brassica rapa ssp chinensis). Wang, Aihua,Huang, Xingxue,Li, Xia,Zhou, Guolin,Hu, Jihong,Hu, Jihong,Yan, Zhixiang. 2016

[18]Impact of sulfate nutrition on the utilization of atmospheric SO2 as sulfur source for Chinese cabbage. Yang, Liping,Stulen, Ineke,De Kok, Luit J..

[19]Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering. Song, Mei-Fang,Shang, Hong-Zhong,Gu, Hai-Ke,Song, Mei-Fang,Zhang, Shu,Li, Jing-Juan,Gao, Jian-Wei,Song, Mei-Fang,Hou, Pei,Guo, Lin,Su, Liang,Yang, Jian-Ping,Xiao, Yang.

[20]IDENTITIES AND RELATIONSHIPS AMONG CHINESE VEGETABLE BRASSICAS AS DETERMINED BY RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS. REN, JP,MCFERSON, JR,LI, RG,KRESOVICH, S,LAMBOY, WF.

作者其他论文 更多>>