数字农科院2.0

Hollow Double-Layered Mesoporous Silica as an Enzyme Immobilization Platform: Enhancing Biocatalytic Efficiency in Docosahexaenoic Acid Phosphatidylcholine-Phosphatidylcholine

文献类型： 外文期刊

作者： Li, Xuefeng;Wei, Jun;Zhang, Yufei;Xu, Yuanzhi;Yuan, Wenlong;Mei, Lin;Yu, Shengjiao;Zheng, Mingming

作者机构：

关键词： immobilized enzyme;hollow double-layered structure;selective etching;bifunctionally modification;structural phospholipid

期刊名称： ACS APPLIED MATERIALS & INTERFACES

ISSN： 1944-8244

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： This study reports the synthesis of bifunctional hollow double-layered mesoporous silica (HdlMS) nanoparticles as an effective support for enzyme catalysis. Using a self-template method combined with sol-gel processing, selective etching, and surface modification with alkyl groups and nitrogenous fatty amine groups, we successfully prepared the NH2/C8-HdlMS carrier. Phospholipase A1 was then immobilized onto this carrier to construct PLA1@NH2/C8-HdlMS, which was subsequently applied to the synthesis of docosahexaenoic-acid-enriched phosphatidylcholine. The results demonstrated a docosahexaenoic acid incorporation rate of 67% within 8 h, and a catalytic efficiency (CE) of 18.84 mmolg-1h-1, the highest value reported to date. The biocatalyst retained approximately 81% of its initial activity after nine consecutive cycles, demonstrating excellent stability and reusability. The HdlMS structure, with its large internal surface area and optimized substrate transport, combined with surface modifications that enhance enzyme-support interactions, contributed to the high CE value, as confirmed by the detailed controlled experiments. This study not only offers novel design principles for high-performance enzyme immobilization carriers but also advances the development of structurally tailored phospholipids for industrial applications.

分类号：

  • 相关文献

[1]Hollow Mesoporous Carbon-Based Enzyme Nanoreactor for the Confined and Interfacial Biocatalytic Synthesis of Phytosterol Esters. Zhang, Shan,Hou, Huaqing,Zhao, Baozhu,Zhou, Qi,Tang, Rongfeng,Chen, Lin,Mao, Jin,Deng, Qianchun,Zheng, Lei,Shi, Jie. 2023

[2]Hollow Hierarchical Porous and Antihydrolytic Spherical Zeolitic Imidazolate Frameworks for Enzyme Encapsulation and Biocatalysis. Zhao, Baozhu,Yang, Haowen,Mao, Jin,Zhou, Qi,Deng, Qianchun,Zheng, Lei,Shi, Jie. 2024

[3]Ultrasound-assisted intensification of Pickering interfacial biocatalysis preparation of vitamin A aliphatic esters. Yufei Zhang,Yundong Lai,Mingming Zheng. 2024

[4]Broadly adapted and efficient enzymatic transesterification production of medium and long-chain triglycerides via coconut oil and long-chain triacylglycerols. Chuang Yang,Yufei Zhang,Yuanzhi Xu,Yibin Zhou,Shiyi Li,Mingming Zheng. 2025

[5]Enzymatic synthesis of antioxidant peptides with controllable and adjustable molecular weights using magnetically recyclable immobilized Alcalase. Junhua Di,Yang Li,Yi Zhang,Kheng Lim Goh,Mingming Zheng. 2025

[6]Enhanced Pickering interfacial biocatalysis in phosphatidylserine synthesis via phospholipase D immobilization with optimized bifunctional strategy. Huihui Liu,Yihan Liu,Zhongmu Han,Yufei Zhang,Mingming Zheng. 2025

作者其他论文 更多>>