数字农科院2.0

Precise Determination of Selenium Forms and Contents in Selenium-Enriched Rapeseed Seedlings and Flowering Stalks by HPLC-ICP-MS

文献类型: 外文期刊

作者: Zhan, Jie-Peng;Qi, Meng;Wang, Chao;Wang, Xin-Fa;Wang, Han-Zhong;Dun, Xiao-Ling

作者机构:

关键词: selenium-enriched plant;rapeseed;seleniumform;methylselenocysteine;HPLC-ICP-MS

期刊名称: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN: 0021-8561

年卷期: 2024 年

页码:

收录情况: SCIE(2024版) ; ; EI(2024版)

摘要: Rapeseed (Brassica napus L.) has the ability of selenium (Se) enrichment. Identification of selenides in Se-rich rapeseed products will promote the development and utilization of high value. By optimizing the Se species extraction process (protease type, extraction reagent, enzyme sample ratio, extraction time, etc.) and chromatographic column, an efficient, stable, and accurate method was established for the identification of Se species and content in rapeseed seedlings and flowering stalks, which were cultured by inorganic Se hydroponics. Five Se compounds, including selenocystine (SeCys(2)), methylselenocysteine (MeSeCys), selenomethionine (SeMet), selenite (SeIV), and selenate (SeVI) were qualitatively and quantitatively identified. Organoselenium was absolutely dominant in both seedlings and flowering stalks among the detected rapeseed varieties, with 64.18-90.20% and 94.38-98.47%, respectively. Further, MeSeCys, a highly active selenide, predominated in rapeseed flowering stalks with a proportion of 56.36-72.93% and a content of 1707.3-5030.3 mu g/kg. This study provides a new source of MeSeCys supplementation for human Se fortification.

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