数字农科院2.0

Identification of miR-71* as a regulator of Flotillin-1 in egg development of Rhipicephalus sanguineus

文献类型： 外文期刊

作者： Qilin Wang;Muxiao Li;Wenge Liu;Yanlong Wang;Zeyu Chen;Zhenjie Zhang;Guangyuan Liu;Meng Qi

作者机构：

关键词： Flotillin-1;MicroRNAs;Rhipicephalus sanguineus;Tick egg

期刊名称： Veterinary Research Communications

ISSN： 0165-7380

年卷期： 2025 年 49 卷 3 期

页码：

收录情况： SCIE（2025版）

摘要： Identifying the microRNAs (miRNAs) that regulate the growth and development of Rhipicephalus sanguineus (Rh. sanguineus) is essential for developing effective control strategies against both the tick and tick-borne pathogens. This study reveals the antagonistic regulatory mechanism between miR-71* (the passenger strand of miRNA-71) and Flotillin-1 in the egg development of Rh. sanguineus. Through bioinformatics prediction and validation using a dual-luciferase reporter system, we demonstrate for the first time that miR-71* directly targets and regulates Flotillin-1. Functional experiments indicate that inhibiting miR-71* significantly increases tick egg weight, while suppressing Flotillin-1 reduces egg weight, thereby suggesting that these molecules negatively regulate egg developmental processes. This discovery provides a novel target for elucidating reproductive regulatory pathways in ticks and lays a theoretical foundation for developing control strategies against ticks and tick-borne pathogens.

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