数字农科院2.0

Customized optimization of lignocellulolytic enzyme cocktails for efficient conversion of pectin-rich biomass residues

文献类型： 外文期刊

作者： Gao L.;Liu G.;Zhao Q.;Xiao Z.;Sun W.;Hao X.;Liu X.;Zhang Z.;Zhang P.

作者机构：

关键词： Biomass valorization;Cellulase;Pectinase;Trichoderma reesei

期刊名称： Carbohydrate Polymers

ISSN： 0144-8617

年卷期： 2022 年 297.0 卷

页码：

收录情况： SCIE（2022版） ; EI（2022版）

摘要： Pectin is a major component in many agricultural feedstocks. Despite the wide use in industrial production of cellulases and hemicellulases, the fungus Trichoderma reesei lacks a complete enzyme set for pectin degradation. In this study, three representative pectinolytic enzymes were expressed and screened for their abilities to improve the efficiency of T. reesei enzymes on the conversion of different agricultural residues. By replacing 5 % of the T. reesei proteins, endopolygalacturonase and pectin lyase remarkably increased the release of sugars from inferior tobacco leaves. In contrast, pectin methylesterase showed the strongest improving effect (by 31.1 %) on the hydrolysis of beetroot residue. The pectin in beetroot residue was only mildly degraded with the supplementation of pectin methylesterase, which allowed the extraction of pectin keeping the original emulsifying activity with a 51.1 % higher yield. The results provide a basis for precise optimization of lignocellulolytic enzyme systems for targeted valorization of pectin-rich agricultural residues. © 2022 Elsevier Ltd

分类号：

  • 相关文献

[1]Revisiting overexpression of a heterologous beta-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities. Xue, Xianli,Ma, Rui,Luo, Huiying,Su, Xiaoyun,Yao, Bin,Wu, Yilan,Qin, Xing. 2016

[2]Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei. Xianhua Sun,Yazhe Liang,Yuan Wang,Honglian Zhang,Tong Zhao,Bin Yao,Huiying Luo,Huoqing Huang,Xiaoyun Su. 2022

[3]A closed-loop strategy for on-site production of saccharolytic enzymes for lignocellulose biorefinery using internal lignocellulosic hydrolysates. Qinqin Zhao,Zheng Zhang,Zhihao Liu,Huiqi Liang,Liwei Gao,Jian Zhao,Guodong Liu,Yinbo Qu. 2024

[4]Recent advances in mechanochemical production of chemicals and carbon materials from sustainable biomass resources. Qi, Xinhua,Yang, Jirui,Qi, Xinhua,Fu, Junyan,Qiu, Mo,Shen, Feng,Xiong, Xinni,Tsang, Daniel C. W.. 2020

[5]Enhanced valorization of hemp stalk via chemo-catalytic and hydrothermal conversions. Yaqi Duan,Zhenhan Tao,Aiguo Zhu,Christophe Len,Yantao Wang,Weiran Yang. 2022

[6]Pectinase production by Aspergillus niger using wastewater in solid state fermentation for eliciting plant disease resistance. Bai, ZH,Zhang, HX,Qi, HY,Peng, XW,Li, BJ.

[7]In vitro study of the interaction between pectinase and chelate-soluble pectin in postharvest apricot fruits. Chen, Yamin,Chen, Fusheng,Lai, Shaojuan,Liu, Kunlun,Bu, Guanhao,Yang, Hongshun,Liu, Hui,Deng, Yun. 2013

[8]Induced resistance to Cladosporium cucumerinum in cucumber by pectinases extracted from Penicillium oxalicum. Peng, XW,Zhang, HX,Bai, ZH,Li, BJ. 2004

[9]Effect Of Pectin Osmosis Or Degradation On The Water Migration And Texture Properties Of Apple Cube Dried By Instant Controlled Pressure Drop Drying (Dic). Li, X, Bi, JF, Jin, X, Li, X, Zhao, YY, Song, Y. 2020

[10]A novel major facilitator transporter TrSTR1 is essential for pentose utilization and involved in xylanase induction in Trichoderma reesei. Huang, Zhen-Bang,Chen, Xiu-Zhen,Qin, Li-Na,Wu, Hong-Qing,Dong, Zhi-Yang,Huang, Zhen-Bang,Su, Xiao-Yun.

[11]Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei. Wu, Yilan,Sun, Xianhua,Xue, Xianli,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Wu, Yilan,Xie, Xiangming.

[12]Improving Cellulase Production In Submerged F.ermentation By The Expression O f A Vitreoscilla Hemoglobin In Trichoderma Reesei. Lin, J, Zhang, XM, Song, BR, Xue, W, Su, XY, Chen, XZ, Dong, ZY. 2017

[13]Enhanced Heterologous Expression Of Trichoderma R.eesei Cel5A/Cel6A In Pichia P astoris With Extracellular Co-Expression Of Vitreoscilla Hemoglobin. Sun, FF, Yang, HM, Bai, RH, Fang, X, Wang, F, He, J, Tu, MB. 2018

[14]Overexpressing Key Component Genes Of T.he Secretion Pathway For E nhanced Secretion Of An Aspergillus Niger Glucose Oxidase In Trichoderma Reesei. Wu, YL, Sun, XH, Xue, XL, Luo, HY, Yao, B, Xie, XM, Su, XY. 2017

[15]Exogenous microbial antagonism affects the bioaugmentation of humus formation under different inoculation using Trichoderma reesei and Phanerochaete chrysosporium. Wang L.,Sun K.,Pan S.,Wang S.,Yan Z.,Zhu L.,Yang X.. 2023

[16]Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei. Wangli Ji,Xiaolu Wang,Xiaoqing Liu,Yuan Wang,Fangui Liu,Bo Xu,Huiying Luo,Tao Tu,Wei Zhang,Xinxin Xu,Xiaoyun Su. 2023

[17]Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei. Shanshan Jiang,Yue Wang,Qin Liu,Qinqin Zhao,Liwei Gao,Xin Song,Xuezhi Li,Yinbo Qu,Guodong Liu. 2022

[18]Rnai-Mediated Gene Silencing Of Trcot1 Induces A Hyperbranching Phenotype In Trichoderma Reesei. Gao, F, Li, MZ, Liu, WQ, Bai, YG, Tao, T, Wang, Y, Zhang, J, Luo, HY, Yao, B, Huang, HQ, Su, XY. 2020

[19]Engineering The Cbh1 Promoter Of Trichoderma Reesei For Enhanced Protein Production By Replacing The Binding Sites Of A Transcription Repressor Ace1 To Those Of The Activators. Sun, XH, Zhang, XH, Huang, HQ, Wang, Y, Tu, T, Bai, YG, Wang, YR, Zhang, J, Luo, HY, Yao, B, Su, XY. 2020

[20]Disruption of the Trichoderma reesei gul1 gene stimulates hyphal branching and reduces broth viscosity in cellulase production. Zhao Qinqin,Liu Qin,Wang Qi,Qin Yuqi,Zhong Yaohua,Gao Liwei,Liu Guodong,Qu Yinbo. 2021

作者其他论文 更多>>