数字农科院2.0

GWAS combined with QTL mapping reveals the genetic loci of leaf morphological characters in Nicotiana tabacum

文献类型： 外文期刊

作者： Yan Ji;Guoxiang Liu;Sifan Yan;Xun Jiang;Mengting Wu;Wei Liu;Yuan Li;Aiguo Yang;Peigang Dai;Shuaibin Du;Yangyang Li;Jun Wang;Xingwei Zhang

作者机构：

关键词： Cigar tobacco;GWAS;Leaf morphological traits;QTL mapping

期刊名称： BMC Plant Biology

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Background: Leaf morphology plays a crucial role in photosynthetic efficiency and yield potential in crops. Cigar tobacco plants, which are derived from common tobacco (Nicotiana tabacum L.), possess special leaf characteristics including thin and delicate leaves with few visible veins, making it a good system for studying the genetic basis of leaf morphological characters. Results: In this study, GWAS and QTL mapping were simultaneously performed using a natural population containing 185 accessions collected worldwide and an F2 population consisting of 240 individuals, respectively. A total of 26 QTLs related to leaf morphological traits were mapped in the F2 population at three different developmental stages, and some QTL intervals were repeatedly detected for different traits and at different developmental stages. Among the 206 significant SNPs identified in the natural population using GWAS, several associated with the leaf thickness phenotype were co-mapped via QTL mapping. By analyzing linkage disequilibrium and transcriptome data from different tissues combined with gene functional annotations, 7 candidate genes from the co-mapped region were identified as the potential causative genes associated with leaf thickness. Conclusions: These results presented a valuable cigar tobacco resource showing the genetic diversity regarding its leaf morphological traits at different developmental stages. It also provides valuable information for novel genes and molecular markers that will be useful for further functional verification and for molecular breeding of leaf morphological traits in crops in the future.

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