数字农科院2.0

A novel antimicrobial protein isolated from potato (Solanum tuberosum) shares homology with an acid phosphatase

文献类型： 外文期刊

作者： Yuan, FH;Gao, Y;Liang, CG;Xu, J;Zhang, CL;He, LY

作者机构：

关键词： Antimicrobial protein;Acid phosphatase;Potato;Disease resistance;Plant defense;Expression;Peptides;Tomato;Genes;Solanacearum

期刊名称： BIOCHEMICAL JOURNAL

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年卷期：

页码：

收录情况： SCI

摘要： A novel antimicrobial protein (AP(1)) was purified from leaves of the potato (Solanum tuberosum, variety MS-42.3) with a procedure involving ammonium sulphate fractionation, molecular sieve chromatography with Sephacryl S-200 and hydrophobic chromatography with Butyl-Sepharose using a FPLC system. The inhibition spectrum investigation showed that AP(1) had good inhibition activity against five different strains of Ralstonia solanacearum from potato or other crops, and two fungal pathogens, Rhizoctonia solani and Alternaria solani from potato. The full-length cDNA encoding AP(1) has been successfully cloned by screening a cDNA expression library of potato with an anti-AP(1) antibody and RACE (rapid amplification of cDNA ends) PCR. Determination of the nucleotide sequences revealed the presence of an open reading frame encoding 343 amino acids. At the C-terminus of AP(1) there is an ATP-binding domain, and the N-terminus exhibits 58% identity with an/the acid phosphatase from Mesorhizobium loti. SDS/PAGE and Western blotting analysis suggested that the AP(1) gene can be successfully expressed in Escherichia coli and recognized by an antibody against AP(1). Also the expressed protein showed an inhibition activity the same as original AP(1) protein isolated from potato. We suggest that AP(1) most likely belongs to a new group of proteins with antimicrobial characteristics in vitro and functions in relation to phosphorylation and energy metabolism of plants.

分类号： Q5

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