数字农科院2.0

Rice Cytochrome P450 Protein CYP71P1 Is Required for Heat Stress Tolerance by Regulating Serotonin Biosynthesis and ROS Homeostasis

文献类型： 外文期刊

作者： Xuantong Lv;Xunan Zhao;Fang Wang;Haili Wang;Yanli Zhang;Banpu Ruan;Guojun Dong;Yanchun Yu;Limin Wu;Fei Chen

作者机构：

关键词： Heat tolerance;HTS2;rice (Oryza sativa);ROS homeostasis;serotonin

期刊名称： Plants

ISSN： 2223-7747

年卷期： 2025 年 14 卷 7 期

页码：

收录情况： SCIE（2025版）

摘要： Heat stress is one of the major factors affecting crop growth and yield. However, the molecular mechanisms underlying rice heat stress tolerance remain largely unclear. In this study, we identified and characterized the rice high temperature sensitive 2 (hts2) mutant, which is highly susceptible to heat stress. Map-based cloning revealed that the HTS2 encodes a cytochrome P450 protein (CYP71P1) involved in serotonin biosynthesis. HTS2 is ubiquitously expressed across plant tissues and shows strong upregulation in response to heat stress. The HTS2 mutation significantly impairs basal serotonin synthesis in rice, and the heat-sensitive phenotype of the hts2 mutant is completely rescued by exogenous serotonin supplementation. Compared to the wild type, the hts2 mutant exhibits reduced antioxidant capacity, leading to excessive reactive oxygen species (ROS) accumulation and severe oxidative damage, ultimately reducing heat stress tolerance. Furthermore, disruption of HTS2 significantly affects the rice heat shock response, with the heat-induced expression of HsfA2s and their downstream target genes, such as HSP18.0 (heat shock protein 18.0) and OsAPX2 (ascorbate peroxidase 2), markedly depressed in hts2 mutant. Our results suggest a pivotal role of HTS2 in modulating serotonin metabolism and maintaining ROS homeostasis during heat stress, offering new perspectives on the mechanisms underlying heat tolerance and potential strategies to enhance rice resilience to heat stress.

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