数字农科院2.0

Development and application of an indirect ELISA for detection of antibodies against emerging atypical porcine pestivirus

文献类型： 外文期刊

作者： Hao Song;Xiaowei Gao;Jing Li;Xinying Dong;Yanhui Fu;Lina Shao;Jiaoer Zhang;Hua Ji Qiu;Yuzi Luo

作者机构：

关键词： Atypical porcine pestivirus;E2 protein;Indirect ELISA;Seroprevalence

期刊名称： Virology Journal

ISSN： 1743-422X

年卷期： 2024 年 21 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Background: Atypical porcine pestivirus (APPV) is a newly discovered swine pestivirus, which can cause congenital tremor and high mortality in newborn piglets and subclinical infection in adult pigs, leading to significant impacts on the pig industry. Currently, there is no approved serological method to assess APPV infection status in pig farms. Methods: In this study, the envelope glycoprotein E2 of APPV was highly expressed in suspension HEK293 cells, and further an indirect enzyme-linked immunosorbent assay based on the recombinant E2 protein (E2-iELISA) was developed and evaluated. Results: The reaction parameters of the E2-iELISA were optimized, and the cutoff value was determined to be 0.2 by analyzing S/P values of 165 negative sera against APPV that were confirmed by virus neutralization test (VNT). Specificity test showed that the method had no cross-reaction with other common swine viruses. The E2-iELISA was evaluated using a panel of swine sera, and showed high sensitivity (113/120, 94.2%) and specificity (65/70, 92.9%), and the agreement rate with VNT was 93.7% (178/190). Subsequently, the E2-iELISA was utilized to investigate the seroprevalence of APPV in pig herds of China. When detecting 1368 pig serum samples collected from nine provinces in China, the overall seroprevalence of APPV was 73.9% (1011/1368). Conclusion: Our findings suggest that the E2-iELISA is specific and sensitive, and could be a valuable tool for serological surveillance of APPV infection in pigs.

分类号：

  • 相关文献

[1]Expression of BVDV E2 protein in CHO-S cells and development of an indirect ELISA for serological detection. Jiahui Wang,Ao Zhang,Zhiwei Hou,Bin Tan,Shuqin Zhang. 2025

[2]Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein. . 2020

[3]Generation, Identification And Functional Analysis O.f Monoclonal Antibodies Against A typical Porcine Pestivirus Ns3 Protein. Yan, M, Liu, G. 2019

[4]Analysis of murine B-cell epitopes on Eastern equine encephalitis virus glycoprotein E2. Sun EnCheng,Zhao Jing,Yang Tao,Xu QingYuan,Qin YongLi,Wang WenShi,Wei Peng,Sun Liang,Sun Jing,Wu DongLai. 2013

[5]Specific ligands for classical swine fever virus screened from landscape phage display library. Yin, Long,Luo, Yuzi,Du, Min,Qiu, Hua-Ji,Yin, Long,Liang, Bo,Wang, Fei,Liu, Aihua,Yin, Long,Wang, Fei,Liu, Aihua,Petrenko, Valery A..

[6]Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus. Yuying Cao,Li Yuan,Shunli Yang,Youjun Shang,Bin Yang,Zhizhong Jing,Huichen Guo,Shuanghui Yin. 2022

[7]Identification of two novel T cell epitopes on the E2 protein of classical swine fever virus C-strain. Xiaotian Zhao,Xiao Wang,Mengqi Yuan,Xin Zhang,Xiaoke Yang,Xiangyu Guan,Shuwen Li,Jifei Ma,Hua Ji Qiu,Yongfeng Li. 2023

[8]Ferritin Nanoparticle Delivery of the E2 Protein of Classical Swine Fever Virus Completely Protects Pigs from Lethal Challenge. Dailang Zhong,Zhanhao Lu,Yu Xia,Hongxia Wu,Xinyu Zhang,Mingzhi Li,Xin Song,Yanjin Wang,Assad Moon,Hua Ji Qiu,Yongfeng Li,Yuan Sun. 2024

[9]A recombinant pseudorabies virus surface - displaying the classical swine fever E2 protein induces specific antibodies rapidly. Zhang, Xinyu,Wu, Hongxia,Gao, Tianqi,Li, Yongfeng,Zhong, Dailang,Li, Mingzhi,Li, Shuwen,Ma, Caoyuan,Moon, Assad,Fu, Qiang,Qiu, Hua-Ji,Sun, Yuan. 2024

[10]Lipid nanoparticle encapsulated membrane-anchored E2 mRNA vaccine elicits cross-protective immune responses against bovine viral diarrhoea virus infection in calves. Ting Le,Bin Jia,Chao Sun,Yongfei Zhou,Yinglin Qi,Hao Liu,Haiqiao Bian,Chongyu Tian,Jun Wang,Fei Xue,Yuanmao Zhu,Jitao Chang,Yunfei Luan,Zexin Zhang,Zhendong Li,Wanbo Tai,Zhigang Jiang,Xin Yin. 2025

[11]A single-dose mRNA vaccine encoding the classical swine fever virus E2-ECD induces durable protective immunity in rabbits. Bian, Li-Jun,Tang, Yu,Yang, Fan,Tian, Hong,Peng, Qin,Tang, Ming-Liang,Chen, Yi-Zhen,Xia, Tian,Li, Shu,Zheng, Hai-Xue,Shu, Hong-Bing,Li, Mi. 2025

[12]E2-based mRNA vaccine encapsulated in lipid nanoparticles protects pigs against classical swine fever virus. Liu, Jingyi,Xia, Yingju,Tian, Chuanwen,Chen, Ziyu,Guo, Weiqiang,Liu, Yingnan,Wen, Jing,Xie, Zhenhua,Lin, Jinzhong,Li, Jiaxin,Chen, Hongjun,Liu, Yebing. 2025

[13]Development and application of G(1)-ELISA for detection of antibodies against bovine ephemeral fever virus. Zheng, Fu-ying,Lin, Guo-zhen,Qiu, Chang-qing,Zhou, Ji-zhang,Cao, Xiao-an,Gong, Xiao-wei. 2009

[14]Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV. Qiu Chang-Qing,Hu Hui,Cao Xiao-An,Zhou Ji-Zhang,Lin Guo-Zhen,Hu Hui. 2008

[15]Detection of the Soybean Allergenic Protein Gly m Bd 28K by an Indirect Enzyme-Linked Immunosorbent Assay. Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua,Liu, Bin,Teng, Da,Wang, Xiumin,Wang, Jianhua.

[16]Prokaryote expression of HPeV-1 VP1 protein, production of VP1 polyclonal antibody and the development of an ELISA. Yu, Ying,Shan, Tongling,Zhu, Caixia,Hua, Xiuguo,Shan, Tongling,Wang, Chunmei,Zhu, Shaofeng,Wang, Chunmei.

[17]Expression of the soybean allergenic protein P34 in Escherichia coli and its indirect ELISA detection method. Liu, Bin,Teng, Da,Wang, Xiumin,Yang, Yalin,Wang, Jianhua,Liu, Bin,Teng, Da,Wang, Xiumin,Yang, Yalin,Wang, Jianhua. 2012

[18]Development of an indirect ELISA for serological detection of reticuloendotheliosis virus using the gp90 protein expressed in Pichia pastoris. Li, Kai,Gao, Honglei,Gao, Li,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei.

[19]An indirect ELISA for detection of Theileria spp. antibodies using a recombinant protein (rTlSP) from Theileria luwenshuni. He, Haining,Li, Youquan,Liu, Junlong,Liu, Zhijie,Yang, Jifei,Liu, Aihong,Chen, Ze,Ren, Qiaoyun,Guan, Guiquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong.

[20]A novel fusion protein-based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis. Liu, Siguo,Guo, Sheping,Wang, Chunlai,Shao, Meili,Zhang, Xiuhua,Guo, Yang,Gong, Qiang. 2007

作者其他论文 更多>>