数字农科院2.0

The tRNA thiolation-mediated translational control is essential for plant immunity

文献类型： 外文期刊

作者： Xueao Zheng;Hanchen Chen;Zhiping Deng;Yujing Wu;Linlin Zhong;Chong Wu;Xiaodan Yu;Qiansi Chen;Shunping Yan

作者机构：

关键词： （1-1-4）A. thaliana;Arabidopsis;NPR1;plant biology;plant immunity;translation;tRNA thiolation

期刊名称： eLife

ISSN： 2050-084X

年卷期： 2024 年 13 卷

页码：

收录情况： SCIE（2024版）

摘要： Plants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections. However, how the translation system contributes to plant immunity is largely unknown. The evolutionarily conserved thiolation modification of transfer RNA (tRNA) ensures efficient decoding during translation. Here, we show that tRNA thiolation is required for plant immunity in Arabidopsis. We identify a cgb mutant that is hyper-susceptible to the pathogen Pseudomonas syringae. CGB encodes ROL5, a homolog of yeast NCS6 required for tRNA thiolation. ROL5 physically interacts with CTU2, a homolog of yeast NCS2. Mutations in either ROL5 or CTU2 result in loss of tRNA thiolation. Further analyses reveal that both transcriptome and proteome reprogramming during immune responses are compromised in cgb. Notably, the translation of salicylic acid receptor NPR1 is reduced in cgb, resulting in compromised salicylic acid signaling. Our study not only reveals a regulatory mechanism for plant immunity but also uncovers an additional biological function of tRNA thiolation.

分类号：

  • 相关文献

[1]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[2]A pair of light signaling factors FHY3 and FAR1 regulates plant immunity by modulating chlorophyll biosynthesis. Wang, Wanqing,Tang, Weijiang,Ma, Tingting,Lin, Rongcheng,Niu, De,Jin, Jing Bo,Wang, Haiyang,Lin, Rongcheng. 2016

[3]New insights into defense responses against Verticillium dahliae infection revealed by a quantitative proteomic analysis in Arabidopsis thaliana. Wu, Min,Li, Qiulin,Xia, Guixian,Zhang, Yongshan,Wang, Fuxin. 2022

[4]四种甘蓝雄性不育类型基因芯片表达谱分析. 康俊根,王晓武,方智远. 2007

[5]NPR1(nonexpresser of pathog-enesis-related genes)调控植物抗病机制的研究进展（英文）. 刘琳琳,江振兴,甄军波,蔡肖,刘迪,张建宏,张香云,迟吉娜. 2017

[6]香蕉NPR1基因片段的克隆及对水杨酸的早期应答反应. 李敏,李胜军,裴新梧. 2007

[7]Identification of the core c-di-GMP turnover proteins responsible for root colonization of Bacillus velezensis. Dong X.,Tu C.,Liu Y.,Zhang R.,Liu Y.. 2022

[8]Regulation of hierarchical carbon substrate utilization, nitrogen fixation, and root colonization by the Hfq/Crc/CrcZY genes in Pseudomonas stutzeri. Fanyang Lv,Yuhua Zhan,Wei Lu,Xiubin Ke,Yahui Shao,Yiyuan Ma,Juan Zheng,Zhimin Yang,Shanshan Jiang,Liguo Shang,Yao Ma,Lei Cheng,Claudine Elmerich,Yongliang Yan,Min Lin. 2022

[9]Secondary metabolites in host pears defense against two fruit borers and cytochrome-P450-mediated counter-defense. Shi Pan Zhang,Bing Bai,Gao Man Chen,Ya Qi Wang,Chao Hu,Xu Fei Liu,Ping Gao,Yu Ting Li,Nan Xia Fu,Xue Qing Yang. 2024

[10]An effector of Phthorimaea absoluta oral secretions inhibits host plant defense. Xiaodi Wang,Xuqing Luo,Jianyang Guo,Nianwan Yang,Fanghao Wan,Zhichuang Lü,Wanxue Liu. 2024

[11]OsNF-YB7 inactivates OsGLK1 to inhibit chlorophyll biosynthesis in rice embryo. Zongju Yang,Tianqi Bai,Zhiguo E,Baixiao Niu,Chen Chen. 2024

[12]Opportunities and challenges in the application of spatiotemporal transcriptomics in plant research. Deng, Peilei,Huang, Jiaruo,He, Wencan,Li, Zhiyuan,Guo, Cun,Chen, Guoxin,Li, Xiaoxu,Zhong, Kejun,Luo, Wei,Kong, Bo. 2025

[13]Structural and deleterious burdens and their effects on yield traits in foxtail millet domestication. Mengrui Du,Fan Zhang,Xu Wang,Tianhao Zhang,Xuanwen Yang,Yuting Liu,Yingchun Zhang,Ting Hou,Guizhou Hang,Xinyue Fang,Jiacui Li,Hui Xue,Yongfeng Zhou,Jiagang Wang. 2025

[14]Expressional divergence of insect GOX genes: From specialist to generalist glucose oxidase. Wang, Xiongya,Gu, Shaohua,Li, Xianchun,Yang, Lihong,Bai, Sufen,Li, Xin,Li, Xianchun,Li, Xianchun,Wang, Chen-Zhu.

[15]Multiple splicing types of OsRIX4, an RAD21 homolog in rice (Oryza sativa L.). Dong HaiTao,Guo XiaoQin,Pei YanXi,Dai ChengEn,Fang YongQi,Tu QiChao,Zhuang JieYun,Zhao Dong,Zheng KangLe,Li DeBao.

[16]Comparative ribosome profiling reveals distinct translational landscapes of salt-sensitive and -tolerant rice. Xiaoyu Yang,Bo Song,Jie Cui,Lina Wang,Shuoshuo Wang,Linlin Luo,Lei Gao,Beixin Mo,Yu Yu,Lin Liu. 2021

[17]Transcriptome-wide analyses of RNA m6A methylation in hexaploid wheat reveal its roles in mRNA translation regulation. Huang, Tao,He, Wei-Jie,Li, Cheng,Zhang, Jing-Bo,Liao, Yu-Cai,Song, Bo,Yang, Peng. 2022

[18]Advances and Breakthroughs in IRES-Directed Translation and Replication of Picornaviruses. Abdullah, Sahibzada Waheed,Wu, Jin'en,Wang, Xuefei,Guo, Huichen,Sun, Shiqi. 2023

[19]Ribosomal control in RNA virus-infected cells. Xiao Wang,Jie Zhu,Da Zhang,Guangqing Liu. 2022

[20]Widespread impact of transposable elements on the evolution of post-transcriptional regulation in the cotton genus Gossypium. Tian, Xuehan,Wang, Ruipeng,Liu, Zhenping,Lu, Sifan,Chen, Xinyuan,Zhang, Zeyu,Liu, Fang,Li, Hongbin,Zhang, Xianlong,Wang, Maojun. 2025

作者其他论文 更多>>