数字农科院2.0

Transcriptome-metabolome analysis reveals how sires affect meat quality in hybrid sheep populations

文献类型： 外文期刊

作者： Bowen Chen;Yaojing Yue;Jianye Li;Jianbin Liu;Chao Yuan;Tingting Guo;Dan Zhang;Bohui Yang;Zengkui Lu

作者机构：

关键词： hybridization;meat quality;metabolomics;sheep;transcriptomics

期刊名称： Frontiers in Nutrition

ISSN： 2296-861X

年卷期： 2022 年 9.0 卷

页码：

收录情况： SCIE（2022版）

摘要： Crossbreeding improves and enhances meat quality and is widely used in sheep production; however, the molecular mechanisms underlying the meat quality of various crossbred sheep remain unknown. In this study, male Southdown, Suffolk and Hu sheep were crossbred with female Hu sheep, and the transcriptomes and metabolomes of the longissimus dorsi muscle of the F1 generation were sequenced to explore how different sire breeds affect meat quality. The results showed that 631 differentially expressed genes and 119 significantly altered metabolites contributed to muscle development characteristics and meat quality-related diversity (P < 0.05). These genes and metabolites were significantly enriched in lipid metabolism pathways, including arachidonic acid metabolism and PPAR signaling. Several candidate genes were associated with muscle growth, such as MYLK3, MYL10, FIGN, MYH8, MYOM3, LMCD1, and FLRT1. Among these, MYH8 and MYL10 participated in regulating muscle growth and development and were correlated with meat quality-related fatty acid levels (|r| > 0.5 and p < 0.05). We selected mRNA from four of these genes to verify the accuracy of the sequencing data via qRT-PCR. Our findings provide further insight into the key genes and metabolites involved in muscle growth and meat quality in hybrid sheep populations.

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