数字农科院2.0

A C-type lectin from domestic silkworm (Bombyx mori) is involved in bacterial recognition and participates in prophenoloxidase activation

文献类型： 外文期刊

作者： Yan Huang;Jing Zhang;Jingyao Shi;Jie Wu;Dan Zhang;Qiaoling Zhao;Dongxu Shen

作者机构：

关键词： Binding;Bombyx mori;C-type lectins;Melanization

期刊名称： Journal of Asia-Pacific Entomology

ISSN： 1226-8615

年卷期： 2026 年 29 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： C-type lectins (CTLs), which belong to the superfamily of glycan-binding proteins, are characterized by the presence of one or more carbohydrate recognition domains (CRDs). CTLs are involved in biological processes, such as immune recognition, intercellular signaling, cell adhesion, and pathogen clearance, and are widely distributed across diverse organisms, from invertebrates to vertebrates. In this study, we investigated the function of the C-type lectin gene named BmIML-2, in the innate immune response of the domestic silkworm, Bombyx mori. First, our results suggested that BmIML-2 was highly expressed in fat body and its transcript levels were significantly up-regulated after induction by different species of bacteria. Afterwards, BmIML-2 was recombinantly expressed using a prokaryotic expression system to delve into its physiological function. Binding assays showed that recombinant BmIML-2 (rBmIML-2) exhibits binding capacity to bacteria, including Escherichia coli, Micrococcus luteus, Staphylococcus aureus and Bacillus subtilis. In addition, Enzyme linked immunosorbent assay (ELISA) demonstrated that rBmIML-2 also binds to cell wall components, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), with more intensely binding affinity to the former. Furthermore, encapsulation assays in vitro suggested that rBmIML-2 was able to accelerate encapsulation and melanization mediated by hemocytes. Finally, we demonstrated that rBmIML-2 significantly potentiates the stimulation pathway of prophenoloxidase (PPO) in plasma. In summary, our results demonstrate that BmIML-2 can act as a pattern recognition receptor (PRR) involved in the host innate immune response.

分类号：

  • 相关文献

[1]Ostrinia furnacalis serpin-3 regulates melanization cascade by inhibiting a prophenoloxidase-activating protease. Zhou, Fan,Hong, Fang,An, Chunju,Liu, Yang,Wang, Guirong.

[2]Serine proteases SP1 and SP13 mediate the melanization response of Asian corn borer, Ostrinia furnacalis, against entomopathogenic fungus Beauveria bassiana. Shen, Dongxu,Hong, Fang,An, Chunju,Liu, Yang,Wang, Guirong.

[3]A Genome-Wide Analysis of Serine Protease Inhibitors in Cydia pomonella Provides Insights into Their Evolution and Expression Pattern. Qiang Wu,Longsheng Xing,Min Du,Cong Huang,Bo Liu,Hongxu Zhou,Wanxue Liu,Fanghao Wan,Wanqiang Qian. 2023

[4]Ecdysone receptor strongly influences larval–pupal–adult transition and melanization in Tuta absoluta. Wang X.,Wu J.,Guo J.,Yang N.,Wan F.,Lü Z.,Liu W.. 2024

[5]Assembling of Holotrichia parallela (dark black chafer) midgut tissue transcriptome and identification of midgut proteins that bind to Cry8Ea toxin from Bacillus thuringiensis. Shu, Changlong,Tan, Shuqian,Yin, Jiao,Geng, Lili,Song, Fuping,Li, Kebin,Zhang, Jie,Soberon, Mario,Bravo, Alejandra,Liu, Chunqing.

[6]Co-expression and preferential interaction between two calcineurin B-like proteins and a CBL-interacting protein kinase from cotton. Gao, Peng,Zhao, Pi-Ming,Wang, Juan,Wang, Hai-Yun,Wang, Gui-Ling,Xia, Gui-Xian,Gao, Peng,Zhao, Pi-Ming,Wang, Juan,Wang, Hai-Yun,Wang, Gui-Ling,Xia, Gui-Xian,Du, Xiong-Ming.

[7]Binding Site Concentration Explains the Differential Susceptibility of Chilo suppressalis and Sesamia inferens to Cry1A-Producing Rice. Han, Chao,Hou, Maolin,Peng, Yufa,Liu, Zewen,Chen, Fajun,Jurat-Fuentes, Juan Luis.

[8]Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides. Liu, YS,Zhang, J,Liu, Q,Zhang, CG,Ma, QS.

[9]Cross-resistance studies of Cry1Ac-resistant strains of Helicoverpa armigera (Lepidoptera : Noctuidae) to Cry2Ab. Luo, Shudong,Wu, Kongming,Tian, Yan,Liang, Gemei,Feng, Xue,Zhang, Jie,Guo, Yuyuan.

[10]The SnRK2-APC/C-TE regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways. Lin, Qibing,Wu, Fuqing,Sheng, Peike,Zhang, Zhe,Zhang, Xin,Guo, Xiuping,Wang, Jiulin,Cheng, Zhijun,Wang, Jie,Wang, Haiyang,Wan, Jianmin,Wan, Jianmin.

[11]Binding chicken Anx2 is beneficial for infection with infectious bursal disease virus. Ren, Xiangang,Zhang, Lizhou,Gao, Yulong,Gao, Honglei,Wang, Yongqiang,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Jiang, Lili,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[12]Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding. Chang Lu,Anand Lopez,Jinkai Zheng,Juewen Liu. 2022

[13]Residues E53, L55, H59, and G70 of the cellular receptor protein Tva mediate cell binding and entry of the novel subgroup K avian leukosis virus. Xinyi Li,Yuntong Chen,Mengmeng Yu,Suyan Wang,Peng Liu,Lingzhai Meng,Ru Guo,Xiaoyan Feng,Mingxue Hu,Tana He,Xiaole Qi,Kai Li,Li Gao,Yanping Zhang,Changjun Liu,Hongyu Cui,Xiaomei Wang,Yulong Gao. 2023

[14]Binding Affinity Of Five Pbps T.o Ostrinia Sex Pheromones. Zhang, TT,Sun, YQ,Wanner, KW,Coates, BS,He, KL,Wang, ZY. 2017

[15]Inhibition of Dipeptidyl Peptidase-4 by Flavonoids: Structure-Activity Relationship, Kinetics and Interaction Mechanism. Pan, Junkun,Zhang, Qiang,Zhang, Chunling,Yang, Wenbo,Liu, Hui,Lv, Zhenzhen,Liu, Jiechao,Jiao, Zhonggao. 2022

[16]Functional Analysis Of A Mirna-Like Small Rna Derived From Bombyx Mori Cytoplasmic Polyhedrosis Virus. Guo, Xi-Jie,Guo, Xi-Jie,Geng, Tao,Jiang, Xiao-Xu,Chen, Tian,Hou, Cheng-Xiang,Hou, Cheng-Xiang,Gao, Kun,Pan, Zhong-Hua,Gao, Kun,Guo, Jian-Yong,Guo, Jian-Yong,Wang, Yong-Sheng,Shang, Meng-Ke,Wang, Yong-Sheng,Wu, Ping,Wu, Ping. 2020

[17]Inhibition Of Heat Shock Protein 9.0 Suppresses Bombyx Mori N ucleopolyhedrovirus Replication In B. Mori. Guo, X. J.,Huang, H. L.,Zhang, S. L.,Tang, X. D.,Guo, X. J.,Wu, P.,Tang, X. D.,Shang, Q.,Wu, P.,Wu, P.. 2019

[18]Expression Analysis And Functional Identification O.f Several Genes Related T o Diapause In Bombyx Mori. Tang, Shunming,Tang, Shunming,Tan, Zhichen,Shen, Xingjia,Qian, Ping,Shen, Xingjia,Chen, Yanhua,Jiang, Tao,Qian, Ping,Li, Jiashuang,Jiang, Tao. 2019

[19]Expression Profile Of Several Genes O.n Ecdysteroidogenic Pathway Related T o Diapause In Pupal Stage Of Bombyx Mori Bivoltine Strain. Tang, Shunming,Zhao, Qiaoling,Huang, Jinshan,Zhu, Juan,Zhao, Qiaoling,Huang, Jinshan,Tang, Shunming,Chen, Yanrong,Shen, Xingjia,Shen, Xingjia. 2019

[20]Transcriptome Sequencing Reveals Potential Mechanisms O.f Diapause Preparation In B ivoltine Silkworm Bombyx Mori (Lepidoptera: Bombycidae). Shen, Xing-jia,Chen, Yan-hua,Jiang, Tao,Shen, Xing-jia,Tan, Zhi-cheng,Chen, Yan-hua,Chen, Yan-rong,Chen, Yan-rong,Hao, Bi-fang,Xie, Yu-chen,Chen, Yan-rong,Jiang, Tao,Tan, Zhi-cheng,Zhu, Juan,Tang, Shun-ming,Xie, Yu-chen,Tang, Shun-ming,Wang, Sheng-peng,Hao, Bi-fang,Huang, Jin-shan,Zhu, Juan,Wang, Sheng-peng,Huang, Jin-shan. 2017

作者其他论文 更多>>