数字农科院2.0

Dynamic Changes in the Antioxidative Defense System in the Tea Plant Reveal the Photoprotection-Mediated Temporal Accumulation of Flavonoids under Full Sunlight Exposure

文献类型： 外文期刊

作者： Zhang, Qunfeng;Ruan, Jianyun;Mumm, Roland;de Vos, Ric C. H.;Liu, Mei-Ya

作者机构：

关键词： Camellia sinensis;Flavonoids;Light exposure;Metabolomics;Photooxidation;Photoprotection

期刊名称： PLANT AND CELL PHYSIOLOGY

ISSN： 0032-0781

年卷期： 2022 年

页码：

收录情况： SCIE（2022版）

摘要： To reveal the mechanisms underlying how light affects flavonoid metabolism and the potential role of flavonoids in protecting against photooxidative stress in tea leaves, tea plants adapted to low-light conditions were exposed to full sunlight over 48 h. There was an increase in the activities of catalase (CAT) and superoxide dismutase (SOD) as well as greater accumulation of reactive oxygen species, lutein, tocopherols, ascorbate and malondialdehyde, suggestive of a time-dependent response to photooxidative stress in tea leaves. Analysis of the time dependency of each element of the antioxidant system indicated that carotenoids and tocopherols exhibited the fastest response to light stress (within 3 h), followed by SOD, CAT and catechin, which peaked at 24 h. Meanwhile, flavonols, vitamin C and glutathione showed the slowest response. Subsequent identification of the main phytochemicals involved in protecting against oxidative stress using untargeted metabolomics revealed a fast and initial accumulation of nonesterified catechins that preceded the increase in flavonol glycosides and catechin esters. Gene expression analysis suggested that the light-induced accumulation of flavonoids was highly associated with the gene encoding flavonol synthase. Ultraviolet B (UV-B) irradiation further validated the time-dependent and collaborative effects of flavonoids in photoprotection in tea plants. Intriguingly, the dynamics of the metabolic response are highly distinct from those reported for Arabidopsis, suggesting that the response to light stress is not conserved across plants. This study additionally provides new insights into the functional role of flavonoids in preventing photooxidative stress and may contribute to further improving tea quality through the control of light intensity.

分类号：

  • 相关文献

[1]Bitterness and astringency of tea leaves and products: Formation mechanism and reducing strategies. Jian Hui Ye,Ying Ye,Jun Feng Yin,Jing Jin,Yue Rong Liang,Ru Yi Liu,Ping Tang,Yong Quan Xu. 2022

[2]Insight into the pigmented anthocyanins and the major potential co-pigmented flavonoids in purple-coloured leaf teas. Jiang Shi,Jesus Simal-Gandara,Jufen Mei,Wanjun Ma,Qunhua Peng,Yali Shi,Qi Xu,Zhi Lin,Haipeng Lv. 2021

[3]Insight into the pigmented anthocyanins and the major potential co-pigmented flavonoids in purple-coloured leaf teas. Jiang Shi,Jesus Simal-Gandara,Jufen Mei,Wanjun Ma,Qunhua Peng,Yali Shi,Qi Xu,Zhi Lin,Haipeng Lv. 2021

[4]Lignin Metabolism Is Crucial in the Plant Responses to Tambocerus elongatus (Shen) in Camellia sinensis L.. Wenli Wang,Xiaogui Zhou,Qiang Hu,Qiuhong Wang,Yanjun Zhou,Jingbo Yu,Shibei Ge,Lan Zhang,Huawei Guo,Meijun Tang,Xin Li. 2025

[5]Revealing the Molecular Regulatory Mechanism of Flavonoid Accumulation in Tender Leaves of Tea Plants by Transcriptomic and Metabolomic Analyses. Ruiyang Shan,Yongheng Zhang,Xiaomei You,Xiangrui Kong,Yazhen Zhang,Xinlei Li,Lu Wang,Xinchao Wang,Changsong Chen. 2025

[6]Metabolomics analysis reveals crucial effects of arbuscular mycorrhizal fungi on the metabolism of quality compounds in shoots and roots of Camellia sinensis L. Shibei Ge,Zheng Zhang,Qiang Hu,Qiuhong Wang,Xuejiao Gong,Fan Huang,Lan Zhang,Wenyan Han,Fan Luo,Xin Li. 2025

[7]Metabolomics and proteomics reveal the molecular basis of colour formation in the pericarp of Chinese wild rice (Zizania latifolia). Yu X.,Qi Q.,Li Y.,Li N.,Xie Y.,Ding A.,Shi J.,Du Y.,Liu X.,Zhang Z.,Yan N.. 2022

[8]Key metabolites and mechanistic insights in forchlorfenuron controlling kiwifruit development. Yingying Bi,Chengkui Qiao,Lijun Han,Hanzhong Xie,Yanjun Xu,Di Wu,Ming Zhuang,Xinru Lv,Mengyuan Cao. 2023

[9]Total Flavonoids Extracts of Apocynum L. from the Ili River Valley Region at Different Harvesting Periods and Bioactivity Analysis. Shao D.,Gao G.,Abubakar A.S.,Hazaisi H.,Chen P.,Chen J.,Chen K.,Wang X.,Wang Y.,Chen Y.,Yu C.,Zhu A.. 2022

[10]Metabolomics analysis unveils important changes involved in the salt tolerance of Salicornia europaea. Duan H.,Tiika R.J.,Tian F.,Lu Y.,Zhang Q.,Hu Y.,Cui G.,Yang H.. 2023

[11]Comparative Analysis of Flavonoids, Carotenoids, and Major Primary Compounds in Site-Specific Yellow-Leaf Tea and Their Dynamic Alterations During Processing. Yang, Jiaqi,Zhou, Qi,Fang, Shitao,Yan, Kangni,Peng, Qunhua,Lin, Zhi,Lv, Haipeng,Mu, Dan,Fu, Jianyu,Shi, Jiang. 2025

[12]Transcriptomic and metabolomic insights into temperature-dependent changes in catechin and anthocyanin accumulation in tea plants with different leaf colors. Jingbo Yu,Qianying Wang,Wenli Wang,Ruihong Ma,Changqing Ding,Kang Wei,Liyuan Wang,Shibe Ge,Yuanzhi Shi,Xin Li. 2024

[13]Analysis of the characteristics of foxtail millet during storage under different light environments. Kehong Liang,Yuhang Liu,Shan Liang. 2021

[14]Genetic analysis of tolerance to photo-oxidative stress induced by high light in winter wheat (Triticum aestivum L.). Li, Hongwei,Tong, Yiping,Li, Bin,Li, Zhensheng,Jing, Ruilian,Lu, Congming. 2010

[15]Evaluation of milk photooxidation based on peptidomics. Sijia Tan,Qingyang Zhang,Xiaoyan Pei,Dongfei Tan,Can Guo,Sumeng Chen,Gang Chen. 2023

[16]Effects of the Oxygen Content and Light Intensity on Milk Photooxidation Using Untargeted Metabolomic Analysis. Dongfei Tan,Aijin Ma,Shaolei Wang,Qingyang Zhang,Man Jia,Afaf Kamal-Eldin,Huaxing Wu,Gang Chen. 2021

[17]Enhanced hole-proton-coupled electron transfer of spin polarized photocatalyst via Mo-S4 coordination for sequential activation of C (sp3)-H and O-H bonds. Wang, Xiaoping,Yang, Bowen,Wang, Haoye,Guo, Haixin,Smith, Richard Lee,Su, Yaqiong,Qi, Xinhua. 2025

[18]Response of photosynthesis to short-term drought stress in rice seedlings overexpressing C-4 phosphoenolpyruvate carboxylase from maize and millet. Ding, Z. S.,Sun, X. F.,Huang, S. H.,Zhou, B. Y.,Zhao, M..

[19]Dynamical Regulation of Photosynthetic Components Related to Photosynthesis and Photoprotection in Maturing Blueberry Fruit. Li, Xiaobai,Pan, Xuhao,Liu, Li,Wen, Xing,Jin, Liang,Yu, Fei,Guo, Weidong. 2023

[20]Recent progress in preventive effect of collagen peptides on photoaging skin and action mechanism. Chongyang Li,Yu Fu,Hongjie Dai,Qiang Wang,Ruichang Gao,Yuhao Zhang. 2022

作者其他论文 更多>>