数字农科院2.0

Development of a competitive ELISA method based on VLPs detecting the antibodies of serotype A FMDV

文献类型： 外文期刊

作者： Yun Zhang;Rui Wang;Manyuan Bai;Xuefei Wang;Hu Dong;Jiajun Li;Suyu Mu;Haisheng Miao;Jianling Song;Shiqi Sun;Huichen Guo

作者机构：

关键词： Competitive ELISA;Foot-and-mouth disease;Virus-like particles

期刊名称： Journal of Virological Methods

ISSN： 0166-0934

年卷期： 2022 年 300 卷

页码：

收录情况： SCIE（2022版）

摘要： Foot-and-mouth disease (FMD) is the highly contagious disease of cloven-hoofed animal that brings considerable economic losses to the animal husbandry. So FMD surveillance which relying on accurate diagnosis is important. Most producing the diagnostic antigen of inactivated FMD virus (FMDV) requires facilities with high biosafety. In our previous studies, virus-like particles(VLPs) resembled the structures of natural virus particles. Here, we established a competitive ELISA (cELISA) method for the detection of antibodies against serotype A FMDV based on serotype A FMDV-VLPs. Via detecting different positive serum and negative serum with different titers, and comparing with different commercial ELISA kits. The specificity and sensitivity of the assay were 100 % and 98 %, respectively. The coincidence rate using the PrioCHECK® FMDV Type A antibody ELISA kit and Liquid-phase blocking (LPB) ELISA were 95.30 % and 92.2 %. Repetitive experiments showed that variation coefficient of intra-batch and inter-batch were less than 9 % and 13 %. The result demonstrated that cELISA based on VLPs from prokaryotic system is highly specific, sensitive and reproducible. The cELISA could also be used to assess the immune responses of serotype A FMDV, especially in developing countries.

分类号：

  • 相关文献

[1]Hollow Mesoporous Silica Nanoparticles As D.elivery Vehicle Of Foot-And-Mouth D isease Virus-Like Particles Induce Persistent Immune Responses In Guinea Pigs. Bai, MY,Dong, H,Su, X,Jin, Y,Sun, SQ,Zhang, YP,Yang, YS,Guo, HC. 2019

[2]Article four simple biomimetic mineralization methods to improve the thermostability and immunogenicity of virus-like particles as a vaccine against foot-and-mouth disease. Mengnan Guo,Jiajun Li,Zhidong Teng,Mei Ren,Hu Dong,Yun Zhang,Jiaxi Ru,Ping Du,Shiqi Sun,Huichen Guo. 2021

[3]Novel capsid-specific single-domain antibodies with broad foot-and-mouth disease strain recognition reveal differences in antigenicity of virions, empty capsids, and virus-like particles. Haozhou Li,Aldo Dekker,Shiqi Sun,Alison Burman,Jeroen Kortekaas,Michiel M. Harmsen. 2021

[4]Evaluation of the immune effect of foot-and-mouth disease virus-like particles derived from Pichia Pastoris on mice and pigs. Li, Zhiyao,Bai, Manyuan,Yin, Shuanghui,Yang, Yan,Dong, Hu,Teng, Zhidong,Sun, Shiqi,Bao, Endong,Guo, Huichen. 2025

[5]Glycosylated Foot-And-Mouth Disease Virus-Like Particles Produced in Pichia Pastoris Enhance Stability and Immunogenicity. Zhiyao Li,Hu Dong,Shuanghui Yin,Manyuan Bai,Zhidong Teng,Lingbo Chen,Suyu Mu,Yun Zhang,Yaozhong Ding,Shiqi Sun,Huichen Guo. 2025

[6]Astragalus Polysaccharide Nanoemulsion: A Promising Adjuvant for Foot-And-Mouth Disease Virus-Like Particle Vaccines. Xiaoni Shi,Zhidong Teng,Kun Yang,Hetao Song,Yun Zhang,Shuzhen Tan,Hu Dong,Shiqi Sun,Yaozhong Ding,Huichen Guo. 2025

[7]Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies. Manyuan Bai,Rui Wang,Shiqi Sun,Yun Zhang,Hu Dong,Huichen Guo. 2021

[8]A new nanobody-enzyme fusion protein–linked immunoassay for detecting antibodies against influenza A virus in different species. Pinpin Ji,Kun Wang,Lu Zhang,Zhenda Yan,Min Kong,Xuwen Sun,Qiang Zhang,Ning Zhou,Baoyuan Liu,En Min Zhou,Yani Sun,Xinjie Wang,Qin Zhao. 2022

[9]Development and Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay Based on Swine Monoclonal Antibodies for Detecting Neutralizing Antibodies against Senecavirus A. Ma, Xueqing,Huang, Jiaxin,Li, Kun,Ding, Kailu,Fu, Yuanfang,Zhang, Jing,Zhao, Zhixun,Li, Pinghua,Bai, Xingwen,Li, Dong,Liu, Xia,Zeng, Qiaoying,Liu, Zaixin,Sun, Pu,Lu, Zengjun. 2023

[10]Development and Validation of a Competitive ELISA Based on Bovine Monoclonal Antibodies for the Detection of Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype A. Yimei Cao,Kun Li,Xiangchuan Xing,Guoqiang Zhu,Yuanfang Fu,Huifang Bao,Xingwen Bai,Pu Sun,Pinghua Li,Jing Zhang,Xueqing Ma,Jian Wang,Zhixun Zhao,Dong Li,Zaixin Liu,Zengjun Lu. 2022

[11]Diagnostic Performance of Competitive ELISA and Western Blot Methods for the Detection of Antibodies against Theileria equi and Babesia caballi. Yang G.,Zhou B.,Chen K.,Hu Z.,Guo W.,Wang X.,Du C.. 2023

[12]Diagnostic performance of a rapid immunochromatographic test for the simultaneous detection of antibodies to Theileria equi and Babesia caballi in horses and donkeys. Frans Jongejan,Cheng Du,Elias Papadopoulos,Valeria Blanda,Santina Di Bella,Vincenza Cannella,Annalisa Guercio,Domenico Vicari,Sharon Tirosh-Levy,Amir Steinman,Gad Baneth,Sanna van Keulen,Iris Hulsebos,Laura Berger,Xiaojun Wang. 2024

[13]Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus. Wang J.,Sun C.,Hu Z.,Wang F.,Chang J.,Gao M.,Ye D.,Jia Q.,Zou H.,Willems L.,Jiang Z.,Yin X.. 2024

[14]Two nanobody-based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus. Xu Chen,Yueting Chang,Lu Zhang,Xinyu Zhao,Zhihan Li,Zhijie Zhang,Pinpin Ji,Qingyuan Liu,Jiakai Zhao,Jiahong Zhu,Baoyuan Liu,Xinjie Wang,Yani Sun,Qin Zhao. 2024

[15]A candidate competitive ELISA based on monoclonal antibody 3A8 for diagnosis of contagious bovine pleuropneumonia. Wu Q.,Ma Z.,Pan Q.,Liu T.,Zhang Y.,Xin J.,Xu Q.. 2024

[16]Development of a competitive ELISA based on the LSDV A33 antigen. Guohua Chen,Xiaobing He,Zhenzhen Gao,Yongxiang Fang,Takele Tesgera Hurisa,Huaijie Jia,Jinlong Tan,Guangqin Zhou,Baoquan Fu,Weike Li,Zhizhong Jing. 2024

[17]Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O. Cao, Yimei,Li, Fengjuan,Xing, Xiangchuan,Zhang, Huiyan,Zhao, Qiongqiong,Sun, Pu,Fu, Yuanfang,Li, Pinghua,Ma, Xueqing,Zhang, Jing,Zhao, Zhixun,Yuan, Hong,Wang, Jian,Wang, Tao,Bao, Huifang,Bai, Xingwen,Li, Dong,Zhang, Qiang,Li, Kun,Lu, Zengjun. 2025

[18]Development of a competitive ELISA using monoclonal antibodies for detecting neutralizing antibodies against Peste-des-petits-ruminants virus. Linjie Hu,Haoyue Xu,Xueliang Zhu,Hongjiao Peng,Ruixue Deng,Yuefeng Sun,Xuelian Meng. 2025

[19]Nanobody-based competitive enzyme-linked immunosorbent assay for detecting antibodies of porcine reproductive and respiratory syndrome virus. Mingxia Sun,Dan Cao,Man Zhao,Xulong Zhu,Yue Sun,Qian Wang,Hongliang Zhang,Yongbo Yang,Xiaoxiao Tian,Haiwei Wang,Xuehui Cai,Tongqing An. 2025

[20]Remapping the spatial distribution of neutralizing sites and their immunodominance on the capsid of different topotypes of FMDV serotype O by site-directed competitive ELISA for detection of neutralizing antibodies. Zhao, Qiongqiong,Li, Fengjuan,Huang, Shulun,Xing, Xiangchuan,Sun, Ying,Li, Pinghua,Bao, Huifang,Fu, Yuanfang,Sun, Pu,Bai, Xingwen,Yuan, Hong,Ma, Xueqing,Zhao, Zhixun,Zhang, Jing,Wang, Jian,Wang, Tao,Li, Dong,Zhang, Qiang,Qian, Ping,Cao, Yimei,Li, Kun,Li, Xiangmin,Lu, Zengjun. 2025

作者其他论文 更多>>