数字农科院2.0

Monitoring invertebrate pests on cowpea crops across China using eDNA metabarcoding

文献类型： 外文期刊

作者： Chen, Qi;Lv, Jun-Xian;Xie, Wen;Wang, Xing;Wei, Shu-Jun;Huang, Guo-Hua

作者机构：

关键词： environmental DNA;metabarcoding;biodiversity monitoring;cowpea;pest management

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： BACKGROUND Monitoring arthropod pests and their natural enemies provides essential information for pest control decisions in agricultural production. Traditional monitoring methods, such as trapping and visual surveys, have practical limitations because of their time-consuming preprocessing steps and the need for knowledgeable taxonomists. We tested the environmental DNA (eDNA) metabarcoding approach for monitoring cowpea (Vigna unguiculata) arthropod pests and their natural enemies across seven climatic sub-regions of China, comparing it with traditional sample collection and morphological identification methods. RESULTS We showed that eDNA metabarcoding reveals a higher species richness of cowpea arthropods than the traditional visual method. The eDNA method detected 163 species, of which 72, 33 and 57 were pests, natural enemies and other insects, respectively, whereas the traditional method only detected 70 species. There were significant differences in alpha- and beta-diversity for arthropods on cowpea in different climatic sub-regions. We also found that longitude, altitude and geographical distance are associated with species diversity, but latitude, mean air temperature and temperature range are not. CONCLUSION This study represents the first use of eDNA metabarcoding to investigate cowpea arthropod communities, providing a novel method for pest monitoring.

分类号：

  • 相关文献

[1]A novel cold-active xylanase gene from the environmental DNA of goat rumen contents: Direct cloning, expression and enzyme characterization. Wang, Guozeng,Luo, Huiying,Wang, Yaru,Huang, Huoqing,Shi, Pengjun,Yang, Peilong,Meng, Kun,Bai, Yingguo,Yao, Bin.

[2]Direct and efficient cloning of full-length genes from environmental DNA by RT-qPCR and modified TAIL-PCR. Huang, Huoqing,Wang, Guozeng,Zhao, Yanyu,Shi, Pengjun,Luo, Huiying,Yao, Bin.

[3]Editorial: Applications of environmental DNA in the aquatic ecosystem management of East Asia. Conghui Liu,Meng Yan,Wei Xiong,Ning Li,Lei Gao. 2024

[4]Different dissipation potential and dietary risk assessment of tristyrylphenol ethoxylates in cowpea ecosystem in China. Li M.,Wang Q.,Li X.,Yue N.,Jin M.,Zheng L.,Wang J.,Jin F.. 2022

[5]Defensive Resistance of Cowpea Vigna unguiculata Control Megalurothrips usitatus Mediated by Jasmonic Acid or Insect Damage. Tao Li,Mingyue Feng,Yuanming Chi,Xing Shi,Zilin Sun,Zhen Wu,Aomei Li,Wangpeng Shi. 2023

[6]Evaluation of 41 Cowpea Lines Sown on Different Dates in Southern China. Dan Gong,Long Jia,Gaoling Luo,Yanhua Chen,Suhua Wang,Lixia Wang. 2023

[7]Parthenogenesis affects interspecific competition between Megalurothrips usitatus and Frankliniella intonsa (Thysanoptera: Thripidae) in changing environment: evidence from life table study. Guo, Ling-Hang,Wu, Sheng-Yong,Gong, Run-Na,Tang, Liang-De. 2023

[8]Neoseiulus mites as biological control agents against Megalurothrips usitatus (Thysanoptera: Thripidae) and Frankliniella intonsa (Thysanoptera: Thripidae) on cowpea crop: laboratory to field. Zhang, Yu-Fei,Zang, Lian-Sheng,Guo, Ling-Hang,Sukhwinder, Singh,Wu, Sheng-Yong,Yang, Xiangbing,Tang, Liang-De. 2024

[9]Residue behavior and quality influence of tolfenpyrad and cyromazine in cowpea during simulated household washing process. Shufan Jiang,Haoyue Li,Xuan Zhu,Shuhuai Li,Mingyue Wang,Defang Xie,Bei Li,Qiong Wu,Xinzhong Zhang,Bingjun Han. 2024

[10]In situ mass spectrometry imaging reveals pesticide residues and key metabolic pathways throughout the entire cowpea growth process. Shufan Jiang,Jingling Lin,Rui Zhang,Qiong Wu,Hongxing Li,Qun Zhang,Mingyue Wang,Longjun Dai,Defang Xie,Yue Zhang,Xinzhong Zhang,Bingjun Han. 2024

[11]The Potential of Using Different Legume Species in a Push-Pull Approach to Manage Spodoptera frugiperda in Maize in China. Bi, Sijia,Wang, Yinhao,Xu, Tingting,Hu, Benjin,Wang, Zhenying,Jatoi, Wajid Ali,Hu, Fei,Xu, Lina. 2024

[12]Multiomics comprehensive analysis of pre-storage low-temperature on cowpea metabolism. Lichun Han,Zhengrong Wang,Qing Wang,Da Wen Sun,Xuelian He,Hongwei Wang,Lili Ma,Chunmei Bai,Christopher B. Watkins,Jinhua Zuo,Yanyan Zheng. 2024

[13]From field to table: Reducing residual toxicity and risk of four pesticides via washing and blanching of cowpea (Vigna unguiculata (L.) Walp.). Shuangwei Zhang,Yajing Guo,Shanshan Zhu,Linlin Guo,Xinglu Pan,Jun Xu,Fengshou Dong,Yongquan Zheng,Xiaohu Wu. 2025

[14]Greenhouse cultivation enhances pesticide bioaccumulation in cowpeas following repeated spraying. Kai Cui,Jian Wang,Guoping Ma,Shuai Guan,Jingyun Liang,Liping Fang,Teng Li,Zhan Dong,Ruiyan Ding,Xiaohu Wu,Yongquan Zheng. 2024

[15]Evaluation and Validation of Colloidal Gold Immunochromatographic Qualitative Testing Products for the Detection of Emamectin Benzoate, Isocarbophos, and Fipronil in Cowpea Samples. Song, Anning,Wang, Miao,She, Yongxin,Jin, Maojun,Cao, Zhen,Abd El-Aty, A. M.,Wang, Jing. 2025

[16]Identification and Analysis of WRKY Transcription Factors in Response to Cowpea Fusarium Wilt in Cowpea. Yali Hao,Rui Liu,Zhenchuan Mao,Qihong Yang,Shijie Zheng,Xiaofei Lu,Yuhong Yang,Bingyan Xie,Jianlong Zhao,Yan Li,Guohua Chen,Jian Ling. 2024

[17]Field-Based Evaluation of Insecticide Effectiveness on Megalurothrips usitatus in Guangdong, China: Implications for Pest Control Strategies. Peng, Zhengke,Li, Mengni,Guo, Chaosong,Zheng, Huixin,Wu, Mingyue,Yin, Fei,Xiao, Yong,Wang, Huanhuan,Kong, Xiangyi,Zalucki, Myron P.,Xie, Wen,Li, Zhenyu. 2025

[18]Volatiles Emitted from Tea Plants Infested by Ectropis obliqua Larvae Are Attractive to Conspecific Moths. Sun, Xiao-Ling,Wang, Guo-Chang,Gao, Yu,Zhang, Xin-Zhong,Xin, Zhao-Jun,Chen, Zong-Mao,Sun, Xiao-Ling,Gao, Yu,Zhang, Xin-Zhong,Xin, Zhao-Jun,Chen, Zong-Mao,Wang, Guo-Chang,Gao, Yu.

[19]Propagule pressure and colony social organization are associated with the successful invasion and rapid range expansion of fire ants in China. Shoemaker, DeWayne,Yang, Chin-Cheng,Yang, Chin-Cheng,Shih, Cheng-Jen,Luo, Li-Zhi,Shao, Jing-Guo.

[20]A 36-bp deletion in the alpha subunit of glutamate-gated chloride channel contributes to abamectin resistance in Plutella xylostella. Liu, Feng,Shi, Xiuzhen,Wu, Qingjun,Xu, Baoyun,Xie, Wen,Wang, Shaoli,Zhang, Youjun,Liang, Yanpo,Liu, Feng,Liu, Nannan.

作者其他论文 更多>>