数字农科院2.0

A recombinant Fc-fused nanobody provides complete protection against feline parvovirus infection

文献类型： 外文期刊

作者： Sun, Yajie;Li, Tiantian;Liu, Jiajia;Guo, Dingrui;Xu, Liwen;Hu, Bo;Zeng, Haifeng;Cao, Wenyu;Deng, Xiaoyu;Ji, Zhi;Zhang, Chengqi;Bai, Xue

作者机构：

关键词： Feline parvovirus;Nanobody;Molecular docking;Molecular dynamics simulation;Antiviral effect

期刊名称： VETERINARY MICROBIOLOGY

ISSN： 0378-1135

年卷期： 2025 年 312 卷

页码：

收录情况： SCIE（2025版）

摘要： Feline panleukopenia (FPL), caused by feline parvovirus (FPV), is a fatal infectious disease in cats characterized by leukopenia, fever, and severe enteritis, for which no specific antiviral treatment is available. Here, we report the development of a potent neutralizing nanobody-based therapeutic against FPV. A high-diversity camelid VHH library (2.95 x 109 CFU) was constructed and screened to identify Nb35, a nanobody with high affinity and specificity for FPV. To enhance its stability and in vivo persistence, Nb35 was fused to the human IgG1 Fc domain, yielding the recombinant fusion antibody 35Fc. The Fc fusion preserved the favorable biophysical properties of Nb35 while markedly improving pharmacokinetic behavior, enabling sustained viral neutralization. Biochemical and biophysical analyses revealed that 35Fc exhibits strong binding affinity and superior neutralizing potency compared with conventional monoclonal antibodies. Molecular docking and dynamics simulations demonstrated that 35Fc interacts stably with the VP2 capsid protein via an extensive network of hydrogen bonds, salt bridges, and It-cation interactions, with a binding free energy of-63.11 kcal/mol. Key interface residues, including LYS76 and TYR561, etc., play critical roles in maintaining this high-affinity complex. In an FPVinfected cat model, 35Fc treatment achieved 100 % survival, alleviated clinical symptoms, and almost completely suppressed viral replication and shedding across fecal, ocular-nasal, and intestinal tissues, while preserving organ integrity. Collectively, these results demonstrate that 35Fc effectively blocks FPV infection by targeting the VP2 capsid and provide structural and functional evidence for the development of Fc-fused nanobody therapeutics in veterinary medicine.

分类号：

  • 相关文献

[1]Development of a toxin-free competitive immunoassay for aflatoxin M1 based on a nanobody as surrogate calibrator. Chong Cai,Yuan Liu,Xiaoqian Tang,Wen Zhang,Qi Zhang,Peiwu Li. 2023

[2]Molecular Docking and Dynamics Simulation Studies of Ginsenosides with SARS-CoV-2 Host and Viral Entry Protein Targets. Pang Shifeng,Vinothini Boopathi,Mohanapriya Murugesan,Ramya Mathiyalagan,Jong Chan Ahn,Chen Xiaolin,Dong Uk Yang,Gi Young Kwak,Byoung Man Kong,Deok Chun Yang,Se Chan Kang,Zhang Hao. 2022

[3]QSAR model of pancreatic lipase inhibition by phenolic acids and their derivatives based on machine learning and multi-descriptor strategy. Yaqi Liu,Fei Pan,Ou Wang,Zehui Zhu,Qing Li,Zicheng Yang,Wenli Tian,Liang Zhao,Lei Zhao. 2023

[4]A novel High-Affinity Anti-Capsaicinoids monoclonal antibody for High-Sensitivity Immunoassays: Experimental and computational validation. Wen, Weihuan,Pan, Fei,Wang, Zhiruo,Li, Xinyu,Wang, Jiang,Talib, Hafiza Shaiza,Hu, Jie,Zhao, Yupeng,Liu, Zhiwei,Liang, Yifan,Wang, Yupu,Xiong, Wanqi,Luo, Lin,Xu, Zhenlin,Chen, Zhicheng. 2025

[5]Two novel sites determine genetic relationships between CPV-2 and FPV: an epidemiological survey of canine and feline parvoviruses in Changchun, China (2020). Zishu Li,Jiaxi Cai,Chuchu Feng,Yu Wang,Shuren Fang,Xianghong Xue. 2024

[6]A novel feline herpesvirus vector subunit FCV VP1 and FPV VP2 vaccine protects cats against FHV-1 and FPV challenge and induces serum neutralizing antibody responses against FCV. Tang, Aoxing,Li, Bo,Zhu, Meng,Zhu, Shiqiang,Zhang, Da,Li, Na,Zhang, Miao,Zhu, Yingqi,Li, Chuanfeng,Meng, Chunchun,Zhu, Jie,Liu, Guangqing. 2025

[7]Molecular cloning and characterizations of porcine SAMHD1 and its roles in replication of highly pathogenic porcine reproductive and respiratory syndrome virus. Yang, Shen,Shan, Tongling,Zhou, Yanjun,Jiang, Yifeng,Tong, Wu,Liu, Fei,Wen, Feng,Zhang, Qingzhan,Tong, Guangzhi,Shan, Tongling,Zhou, Yanjun,Tong, Guangzhi.

[8]Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo. Chang, Yanyan,Dou, Yongxi,Bao, Huifang,Luo, Xuenong,Liu, Xuerong,Mu, Kebin,Liu, Zaixin,Liu, Xiangtao,Cai, Xuepeng,Chang, Yanyan,Liu, Xuerong,Mu, Kebin,Cai, Xuepeng. 2014

[9]OGG1 inhibition suppresses African swine fever virus replication. Fan, Jie,Lv, Xinqian,Yang, Saixia,Geng, Shuxian,Yang, Jifei,Zhao, Yaru,Zhang, Zhonghui,Liu, Zhijie,Guan, Guiquan,Luo, Jianxun,Zeng, Qiaoying,Yin, Hong,Niu, Qingli. 2023

[10]Lithium Chloride Inhibits Early Stages O.f Foot-And-Mouth Disease Virus ( Fmdv) Replication In Vitro. Zhao, FR, Xie, YL, Liu, ZZ, Shao, JJ, Li, SF, Zhang, YG, Chang, HY. 2017

[11]Serum Exosomes From Newborn Piglets R.estrict Porcine Epidemic Diarrhea V irus Infection. Chen, JN,Jin, L,Yan, MM,Yang, Z,Wang, HW,Geng, SX,Gong, ZL,Liu, GL. 2019

[12]Inhibition of BET Family Proteins Suppresses African Swine Fever Virus Infection. Zhao, Yaru,Niu, Qingli,Yang, Saixia,Yang, Jifei,Zhang, Zhonghui,Geng, Shuxian,Fan, Jie,Liu, Zhijie,Guan, Guiquan,Liu, Zhiqing,Zhou, Jia,Hu, Haitao,Luo, Jianxun,Yin, Hong. 2022

[13]Antiviral Effect of Manganese against Foot-and-Mouth Disease Virus Both in PK15 Cells and Mice. Zhixiong Zhang,Rui Zhang,Juanbin Yin,Shuaiyang Zhao,Xiaodong Qin,Fei Chen,Yang Yang,Ling Bai,Zijing Guo,Yongshu Wu,Yanmin Li,Zhidong Zhang. 2023

[14]S5092 inhibit ASFV infection by targeting the pS273R protease activity in vitro. Chuanxia Liu,Tingting Li,Tao Huang,Gaihong Zhao,Xiao Wang,Jiangnan Li,Li Huang,Zhaoxia Zhang,Jun Zheng,Changjiang Weng. 2025

[15]Diet-Supplemented Probiotic Bacillus velezensis Protects Against Viral Infection Via the Lipopeptide Surfactin in Zebrafish. Wenhao Zhou,Hui Liang,Delong Meng,Shichang Xu,Yuanpei Zhang,He Zhu,Yi Wang,Yalin Yang,Zhen Zhang,Yuanyuan Yao,Xiaoqing Xu,Qianwen Ding,Ming Li,Zhigang Zhou,Chao Ran. 2025

[16]A toxin-free enzyme-linked immunosorbent assay for the analysis of aflatoxins based on a VHH surrogate standard. Wang, Yanru,Li, Peiwu,Zhang, Qi,Hu, Xiaofeng,Zhang, Wen,Wang, Yanru,Li, Peiwu,Hu, Xiaofeng,Zhang, Wen,Li, Peiwu,Zhang, Qi,Li, Peiwu,Zhang, Qi,Hu, Xiaofeng,Li, Peiwu,Zhang, Wen.

[17]Nanobody Technology For Mycotoxin Detection: C.urrent Status And Prospects. He, T, Zhu, J, Nie, Y, Hu, R, Wang, T, Li, PW, Zhang, Q, Yang, YH. 2018

[18]Accelerated Hybridization Chain Reaction Kinetics Using Poly DNA Tetrahedrons and Its Application in Detection of Aflatoxin B1. Xuesong Li,Jie Cheng,Kun Zeng,Shulin Wei,Jiaxuan Xiao,Yanyan Lu,Fang Zhu,Zhanhui Wang,Kun Wang,Xiangyang Wu,Zhen Zhang. 2023

[19]Mapping of foot-and-mouth disease virus antigenic sites recognized by single-domain antibodies reveals different 146S particle specific sites and particle flexibility. Harmsen M.M.,Li H.,Sun S.,van der Poel W.H.M.,Dekker A.. 2023

[20]Identification and characterization of nanobodies specifically against African swine fever virus major capsid protein p72. Yang J.,Jing M.,Niu Q.,Wang J.,Zhao Y.,Liu M.,Guan G.,Luo J.,Yin H.,Liu Z.. 2022

作者其他论文 更多>>