数字农科院2.0

Extracellular Vesicles From Lactobacillus fermentum Enhance Intestinal Barrier Integrity and Restore Gut Microbial Homeostasis in Experimental Murine Colitis

文献类型： 外文期刊

作者： Wang, Yanwei;Li, Tiantian;Dong, Zhuo;Zhang, Qiyue;Mi, Jingqiu;Wang, Qingfeng;Lin, Gang;Ma, Qiugang;Jia, Ru;Huang, Shimeng

作者机构：

关键词： L fermentum;extracellular vesicles;colitis;gut microbiome;intestinal barrier integrity

期刊名称： JOURNAL OF NUTRITION

ISSN： 0022-3166

年卷期： 2025 年 155 卷 5 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Lactobacillus fermentum has been shown to improve intestinal health and treat colitis; however, its precise efficacy and mechanisms in inflammatory bowel disease remain unclear. Objectives: This study aimed to evaluate whether L fermentum and its metabolites, extracellular vesicles, and other components could modulate intestinal barrier function and gut microbiota to alleviate dextran sulfate sodium (DSS)-induced colitis in mice. Methods: Forty-eight mice were randomly assigned to 6 groups: control, DSS, L fermentum+DSS group (LF+DSS), heat-inactivated L fermentum+DSS group (LHF+DSS), L fermentum supernatant solution+DSS group (LSF+DSS), and L fermentum extracellular vesicles+DSS group (LEV+DSS). After a 1-wk acclimation, mice were gavaged daily for 3 wk. Fresh cultures, including live (LF+DSS), heat-inactivated (LHF+DSS), supernatant (LSF+DSS), and extracellular vesicles (LEV+DSS), were prepared daily. During the final 7 d, the control group received normal water, and the other groups received 3% DSS. Data were collected daily, followed by sample collection from the mice. Results: In this study, significant reductions (P < 0.05) in body weight changes, disease activity index, intestinal damage, and histology scores were observed in the treatment groups, especially LEV+DSS and LF+DSS. Additionally, compared with the DSS group, colonic mucus secretion, as well as claudin-1 and occludin expression, increased significantly (P < 0.05) in the LEV+DSS and LF+DSS groups, whereas proinflammatory cytokines IL-1/3 and TNF-alpha decreased (P < 0.05) and IL-10 increased (P < 0.05) in the LEV+DSS group. L fermentum and its components significantly regulated gut microbiota alpha-diversity and beta-diversity, affecting overall composition. Linear discriminant analysis effect size analysis revealed an enrichment of beneficial bacteria including Prevotellaceae_UCG-001, Romboutsia, and Ruminococcus species in vesicles significantly downregulated the gene expression of TNF-alpha and IL-1/3, whereas the expression of IL-10 was upregulated, thereby Conclusions: This study reveals that L fermentum alleviates colitis through modulation of the gut microbiota and reinforcement of the intestinal mucosal barrier, with its extracellular vesicles potentially playing a key role in this regulatory process.

分类号：

  • 相关文献

[1]Natural Products: A Dependable Source of Therapeutic Alternatives for Inflammatory Bowel Disease through Regulation of Tight Junctions. Jing Peng,Hao Li,Oladejo Ayodele Olaolu,Saber Ibrahim,Sally Ibrahim,Shengyi Wang. 2023

[2]Chemopreventive effects of nobiletin and its colonic metabolites on colon carcinogenesis. Wu, Xian,Song, Mingyue,Wang, Minqi,Zheng, Jinkai,Gao, Zili,Xu, Fei,Zhang, Guodong,Xiao, Hang,Zheng, Jinkai.

[3]Honey Polyphenols Ameliorate Dss-Induced Ulcerative C.olitis Via Modulating Gut M icrobiota In Rats. Zhao, HA, Cheng, N, Zhou, WQ, Chen, SN, Wang, Q, Gao, H, Xue, XF, Wu, LM, Cao, W. 2019

[4]Effect of Ornithine a-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis. Wang, Tao,Tian, Junquan,Su, Wenxuan,Yang, Fan,Yin, Jie,Jiang, Qian,Li, Yuying,Yao, Kang,Li, Tiejun,Yin, Yulong. 2023

[5]Royal Jelly Protected against Dextran-Sulfate-Sodium-Induced Colitis by Improving the Colonic Mucosal Barrier and Gut Microbiota. Jianying Guo,Baochen Ma,Zixu Wang,Yaoxing Chen,Wenli Tian,Yulan Dong. 2022

[6]Myd88 mediates colitis-and rankl-induced microfold cell differentiation. Yang Li,Shanshan Yang,Xin Huang,Ning Yang,Caiying Wang,Jing Zhao,Zhizhong Jing,Luc Willems,Guangliang Liu. 2022

[7]Butyrate Mitigates Weanling Piglets From Lipopolysaccharide-Induced Colitis By Regulating Microbiota And Energy Metabolism Of The Gut-Liver Axis. Han, YS, Zhao, QY, Tang, CH, Li, Y, Zhang, K, Li, FD, Zhang, JM. 2020

[8]Curcumin Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice Through Regulating Gut Microbiota. Guo, Xiaoxuan,Xu, Ye,Geng, Ruixuan,Qiu, Jing,He, Xiaoyun. 2022

[9]Colonic innate immune defenses and microbiota alterations in acute swine dysentery. Cristina C. Fodor,Janelle Fouhse,Dominique Drouin,Tao Ma,Benjamin P. Willing,Leluo L. Guan,Eduardo R. Cobo. 2022

[10]Chlorogenic acid supplementation alleviates dextran sulfate sodium (DSS)-induced colitis via inhibiting inflammatory responses and oxidative stress, improving gut barrier integrity and Nrf-2/HO-1 pathway. Fan Wan,Xueying Cai,Mengyu Wang,Liang Chen,Ruqing Zhong,Lei Liu,Bao Yi,Fujiang Hou,Hongfu Zhang. 2021

[11]Genistein-Derived ROS-Responsive Nanoparticles Relieve Colitis by Regulating Mucosal Homeostasis. Wentao Fan,Shuo Zhang,Yuting Wu,Tao Lu,Jiwen Liu,Xiuyun Cao,Shuhui Liu,Liping Yan,Xizhi Shi,Guangliang Liu,Chaobo Huang,Suquan Song. 2021

[12]The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis. Li, Shanshan,Huo, Xiaohui,Qi, Yuli,Ren, Duoduo,Li, Zhiman,Qu, Di,Sun, Yinshi. 2022

[13]2′-Fucosyllactose Ameliorates Inflammatory Bowel Disease by Modulating Gut Microbiota and Promoting MUC2 Expression. Qianqian Yao,Linlin Fan,Nan Zheng,Christophe Blecker,Véronique Delcenserie,Huiying Li,Jiaqi Wang. 2022

[14]Integrated microbiomic and metabolomic analyses reveal the mechanisms by which bee pollen and royal jelly lipid extracts ameliorate colitis in mice. Qiangqiang Li,Wenwen Zhang,Enning Zhou,Yuxiao Tao,Miao Wang,Suzhen Qi,Liuwei Zhao,Yonggang Tan,Liming Wu. 2023

[15]Lactobacillus paracasei Jlus66 relieves DSS-induced ulcerative colitis in a murine model by maintaining intestinal barrier integrity, inhibiting inflammation, and improving intestinal microbiota structure. Yu, Fazheng,Wang, Xiaoxu,Ren, Honglin,Chang, Jiang,Guo, Jian,He, Zhaoqi,Shi, Ruoran,Hu, Xueyu,Jin, Yuanyuan,Lu, Shiying,Li, Yansong,Liu, Zengshan,Hu, Pan. 2024

[16]Ginsenoside Rk3 Ameliorates Obesity-Induced Colitis by Modulating Lipid Metabolism in C57BL/6 Mice. Wang, Weimin,Chen, Hongwei,Zhang, Wenyuan,Fan, Daidi,Deng, Jianjun,Yang, Haixia. 2024

[17]Probiotic Bacillus licheniformis ZW3 Alleviates DSS-Induced Colitis and Enhances Gut Homeostasis. Dan Jia,Yingying Li,Yingjie Wang,Yanan Guo,Junlong Liu,Shuaiyang Zhao,Jinming Wang,Guiquan Guan,Jianxun Luo,Hong Yin,Lijie Tang,Youquan Li. 2024

[18]Network Analysis of Gut Microbial Communities Reveals Key Reason for Quercetin Protects against Colitis. Yanan Lv,Jing Peng,Xiaoyu Ma,Zeyi Liang,Ghasem Hosseini Salekdeh,Qunhua Ke,Wenxiang Shen,Zuoting Yan,Hongsheng Li,Shengyi Wang,Xuezhi Ding. 2024

[19]Soy polysaccharide maintains colonic homeostasis to protect from dextran sulphate sodium-induced colitis by modulating gut microbiota and intestinal epithelial regeneration. Nana Zhang,Jianlin Liu,Weiyue Zhang,Xinxin Guo,Shuying Li,Hongtai Zhang,Minjie Wang,Bei Fan,Fengzhong Wang. 2024

[20]Protective effect of a newly probiotic Lactobacillus reuteri LY2-2 on DSS-induced colitis. Yang, Yong,Qiao, Yuyu,Liu, Ge,Yi, Gaoqin,Liu, Hongli,Zhang, Ting,Tong, Mingwei. 2025

作者其他论文 更多>>