数字农科院2.0

Single-cell profiling of African swine fever virus disease in the pig spleen reveals viral and host dynamics

文献类型： 外文期刊

作者： Zixiang Zhu;Ruoqing Mao;Baohong Liu;Huanan Liu;Zhengwang Shi;Kunpeng Zhang;Huisheng Liu;Danyang Zhang;Jia Liu;Zhenxiang Zhao;Kangli Li;Fan Yang;Weijun Cao;Xiangle Zhang;Chaochao Shen;Dehui Sun;Liyuan Wang;Hong Tian;Yi Ru;Tao Feng;Jijun He;Jianhong Guo;Keshan Zhang;Zhonglin Tang;Shilei Zhang;Chan Ding;Jun Han;Haixue Zheng

作者机构：

关键词： African swine fever virus;cellular tropism;host antiviral response;monocytes;single-cell RNA sequencing

期刊名称： Proceedings of the National Academy of Sciences of the United States of America

ISSN： 1091-6490

年卷期： 2024 年 121 卷 10 期

页码：

收录情况： SCIE（2024版）

摘要： African swine fever, one of the major viral diseases of swine, poses an imminent threat to the global pig industry. The high-efficient replication of the causative agent African swine fever virus (ASFV) in various organs in pigs greatly contributes to the disease. However, how ASFV manipulates the cell population to drive high-efficient replication of the virus in vivo remains unclear. Here, we found that the spleen reveals the most severe pathological manifestation with the highest viral loads among various organs in pigs during ASFV infection. By using single-cell-RNA-sequencing technology and multiple methods, we determined that macrophages and monocytes are the major cell types infected by ASFV in the spleen, showing high viral-load heterogeneity. A rare subpopulation of immature monocytes represents the major population infected at late infection stage. ASFV causes massive death of macrophages, but shifts its infection into these monocytes which significantly arise after the infection. The apoptosis, interferon response, and antigen-presentation capacity are inhibited in these monocytes which benefits prolonged infection of ASFV in vivo. Until now, the role of immature monocytes as an important target by ASFV has been overlooked due to that they do not express classical monocyte marker CD14. The present study indicates that the shift of viral infection from macrophages to the immature monocytes is critical for maintaining prolonged ASFV infection in vivo. This study sheds light on ASFV tropism, replication, and infection dynamics, and elicited immune response, which may instruct future research on antiviral strategies.

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