数字农科院2.0

Cloning and characterization of repetitive sequences and development of SCAR markers specific for the P genome of Agropyron cristatum

文献类型： 外文期刊

作者： Wu, M.;Zhang, J. P.;Wang, J. C.;Yang, X. M.;Gao, A. N.;Liu, W. H.;Li, L. H.;Wu, M.;Wang, J. C.;Zhang, X. K.

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期刊名称： EUPHYTICA

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收录情况： SCI

摘要： In previous studies, we successfully transferred the P genome of Agropyron cristatum into wheat using wide hybridization methods. In the current investigation, repetitive sequences were cloned and DNA markers specific for the P genome of A. cristatum were developed. Three P genome-specific markers, designated OPX07-1036, OPX11-817 and OPC05-1539, were identified and isolated using random amplified polymorphic DNAs. The three markers were successfully amplified in all tested materials that contained Agropyron chromatin, such as Agropyron itself and wheat-Agropyron addition lines. These RAPD markers were converted into SCAR markers to be used in detection of P genome chromatin in wheat. In situ probing with fluorescent-labeled marker DNA has shown that they are distributed in all arms of Agropyron hence they will be useful in a variety of studies on introgressions of the P-genome chromatin into wheat.

分类号： S3

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