数字农科院2.0

Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa

文献类型： 外文期刊

作者： Huang, B. L.;Cheng, C.;Zhang, G. Y.;Su, J. J.;Zhi, Y.;Xu, S. S.;Cai, D. T.;Huang, B. Q.;Zhang, X. K.

作者机构：

关键词： Eruca vesicaria subsp sativa;Nicotianamine synthase;Gene cloning

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. In this study, a gene encoding EvNAS was cloned and characterized in E. vesicaria subsp sativa. The full-length EvNAS cDNA sequence contained a 111-bp 5'-untranslated region (UTR), a 155-bp 3'-UTR, and a 966-bp open reading frame encoding 322-amino acid residues. The EvNAS genomic sequence contained no introns, which is similar to previously reported NAS genes. The deduced translation of EvNAS contained a well-conserved NAS domain (1-279 amino acids) and an LIKI-CGEAEG box identical to some Brassica NAS and to the LIRL-box in most plant NAS, which is essential for DNA binding. Phylogenetic analysis indicated that EvNAS was most closely related to Brassica rapa NAS3 within the Cruciferae, followed by Thlaspi NAS1, Camelina NAS3, and Arabidopsis NAS3. A reverse transcription-polymerase chain reaction indicated that EvNAS expression was greatest in the leaves, followed by the flower buds and hypocotyls. EvNAS was moderately expressed in the roots.

分类号：

  • 相关文献

[1]Cloning and characterization of the dehydration-responsive element-binding protein 2A gene in Eruca vesicaria subsp sativa. Huang, B. L.,Li, Y. Y.,Li, D. Y.,Ma, M. Y.,Cai, D. T.,Wu, W. H.,Huang, B. Q.,Zhang, X. K.. 2016

[2]Nicotianamine synthase gene family as central components in heavy metal and phytohormone response in maize. Zhou, Mei-Liang,Qi, Lei-Peng,Zhang, Qian,Shao, Ji-Rong,Zhou, Mei-Liang,Pang, Jun-Feng,Lei, Zhi,Tang, Yi-Xiong,Wu, Yan-Min,Zhou, Mei-Liang,Zhu, Xue-Mei.

[3]Genome-wide identification, classification and expression profiling of nicotianamine synthase (NAS) gene family in maize. Zhou, Xiaojin,Zhao, Qianqian,Liu, Xiaoqing,Zhang, Shaojun,Sun, Cheng,Fan, Yunliu,Zhang, Chunyi,Chen, Rumei,Zhou, Xiaojin,Zhao, Qianqian,Liu, Xiaoqing,Zhang, Shaojun,Sun, Cheng,Fan, Yunliu,Zhang, Chunyi,Chen, Rumei,Li, Suzhen. 2013

[4]Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge). Cheng, C.,Hu, J.,Zhi, Y.,Su, J. J.,Huang, B. Q.,Zhang, X. K.. 2015

[5]Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Liu, JM,Cai, XZ,Lin, JJ,Fu, ZQ,Yang, GZ,Shi, FH,Cai, YM,Shen, W,Taylor, MG,Wu, XF. 2004

[6]BioVector, a flexible system for gene specific-expression in plants. Wang, Xu,Fan, Chengming,Zhang, Xiaomei,Zhu, Jinlong,Fu, Yong-Fu. 2013

[7]Cloning and Expression Analysis of Two MADS-Box Genes from Strawberry Fruit. Zhou, H. C.,Kang, Z. R.,Zhao, X.,Li, G.,Kang, Z. R.,Li, L..

[8]Cloning and sequence analysis of Alcaligenes faecalis nifHDK gene cluster. Zhang, HY,Lin, M,Xiao, FH,Zhu, HS,Fang, XJ,You, CG,Zhu, YX.

[9]Isolation and characterization of a gene from Medicago sativa L., encoding a bZIP transcription factor. Yang, Qingchuan,Kang, Junmei,Zhang, Tiejun,Sun, Yan,Fang, Feng.

[10]Identification and characterization of a gene encoding a putative lysophosphatidyl acyltransferase from Arachis hypogaea. Chen, Si-Long,Huang, Jia-Quan,Lei, Yong,Zhang, Yue-Ting,Ren, Xiao-Ping,Chen, Yu-Ning,Jiang, Hui-Fang,Yan, Li-Ying,Liao, Bo-Shou,Chen, Si-Long,Li, Yu-Rong,Chen, Si-Long.

[11]Alternative splicing and tissue expression of CIB4 gene in sheep testis. Yu, Yan,Wei, Caihong,Lu, Guobin,Zhang, Ju,Ren, Hangxing,Sheng, Xihui,Du, Lixin,Yu, Yan,Guan, Qun,Zhang, Qiyao,Zhang, Yuan,Wang, Chuduan,Song, Xuemei,Jin, Mei,Li, Shangang. 2010

[12]Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). Jin, YM,Lin, JJ,Feng, XG,Zhang, L,Wu, XF,Zhou, YC,Cai, YM. 2002

[13]Cloning of Schistosoma japonicum Chinese strain fatty acid binding protein (Sj-FABPc) gene and its overproduction in Escherichia coli. Cai, XZ,Tian, E,Yang, GZ,Shi, FH,Shen, W,Cai, YM,Wu, XF. 1998

[14]Cloning of Schistosoma japonicum Chinese strain TPI gene and characterization of its expression product in Escherichia coli. Cai, XZ,Lin, JJ,Yang, GZ,Shi, FH,Shen, W,Cai, YM,Wu, XF. 1998

[15]Cloning and expression of actin gene of Schistosoma japonicum Chinese strain. Zhu, JG,Lin, JJ,Feng, XG,Wu, XF,Zhou, YC,Cai, YM. 2000

[16]The Research on Actinidia eriantha Benth. in South China. Wu, Y. J.,Xie, M.,Zhang, Q. C.,Zhang, H. Q.,Fang, J. B.. 2011

[17]VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Liu, Guanshan,Chen, Jia,Wang, Xuechen. 2006

[18]Purification, gene cloning and characterization of an acidic beta-1,4-glucanase from Phialophora sp G5 with potential applications in the brewing and feed industries. Zhao, Junqi,Shi, Pengjun,Yuan, Tiezheng,Huang, Huoqing,Li, Zhongyuan,Meng, Kun,Yang, Peilong,Yao, Bin. 2012

[19]High-level expression and biochemical characterization of a novel cold-active lipase from Rhizomucor endophyticus. Duan, Xiaojie,Liu, Yu,Jiang, Zhengqiang,Yang, Shaoqing,Zheng, Mingming.

[20]Molecular Cloning, Sequence Analysis, and Expression of the Polygalacturonase-inhibiting Protein (PGIP) Gene in Mulberry. Hu, Dongqing,Dai, Ruiqiang,Wang, Yuhua,Zhang, Yinghua,Liu, Zhaoyue,Zhao, Weiguo,Hu, Dongqing,Dai, Ruiqiang,Wang, Yuhua,Zhang, Yinghua,Liu, Zhaoyue,Fang, Rongjun,Zhao, Weiguo,Li, Long,Li, Liu,Fang, Rongjun,Lin, Qiang.

作者其他论文 更多>>