数字农科院2.0

Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat

文献类型： 外文期刊

作者： Zhang, X. K.;Liu, L.;He, Z. H.;Sun, D. J.;He, X. Y.;Xu, Z. H.;Zhang, P. P.;Chen, F.;Xia, X. C.;Zhang, X. K.;He, Z. H.

作者机构：

关键词： Triticum aestivum L.;quality;multiplex PCR;high molecular weight glutenin subunit;HMW-GS;low molecular weight glutenin subunit;LMW-GS;Wx-B1;Pinb-D1b;PPO activity;1B.1R translocation;marker-assisted selection;MAS

期刊名称： PLANT BREEDING

ISSN： 0179-9541

年卷期： 2008 年 127 卷 2 期

页码：

收录情况： SCI

摘要： Wheat quality properties are genetically determined by the compositions of high and low molecular weight glutenin subunits, grain hardness, polyphenol oxidase (PPO) activity and starch viscosity. Two multiplex PCR assays were developed and validated using 70 cultivars and advanced lines from Chinese autumn-sown wheat regions. Multiplex PCR I includes molecular markers for genes/loci omega-secalin, Glu-B1-2a (By8), Glu-D1-1d (Dx5), Glu-A3d, Glu-B3 (for non-1B.1R type) and Pinb-D1b targeting improved gluten parameters and pan bread quality. Multiplex PCR II comprises markers for genes/loci Ppo-A1, Ppo-D1 and Wx-B1b targeting improved noodle quality. The results were consistent with those achieved by SDS-PAGE and RP-HPLC, indicating that the two multiplex assays were highly effective, with good repeatability and low costs enabling their use in wheat breeding programmes. In total, nine alleles (subunits) at locus Glu-B1, four at Glu-D1 and five at Glu-A3 locus were identified, and the alleles (Subunits) Glu-B1b (7 + 8), Glu-B1c (7 + 9), Glu-D1a (2 + 12), Glu-D1d (5 + 10), Glu-A3a, Glu-A3c and Glu-A3d were most frequently present in the cultivars and lines tested. The 1B.1R translocation was present in 28 (40.0%) lines, whereas the Wx-B1 null allele for better noodle quality was present in only seven (10.0%) cultivars and advanced lines, and 37 (52.9%) lines had Pinb-D1b associated with hard grains. The allele Ppo-A1b on chromosome 2AL associated with lower PPO activity was present in 38 (54.3%) genotypes, whereas the less effective allele Ppo-D1a on chromosome 2DL, also associated with low PPO activity was present in 45 (64.3%) of genotypes. These two multiplex PCR assays should be effective in marker assisted selection targeting improved pan bread-making and noodle qualities.

分类号：

  • 相关文献

[1]Molecular detection of high- and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. H. Jin,J. Yan,R. J. Peña,X. C. Xia,A. Morgounov,L. M. Han,Y. Zhang,Z. H. He. 2011

[2]A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. Liu, Li,Wang, Aili,Ma, Junhong,Lan, Ping,Ma, Wujun,Liu, Li,Xia, Xianchun,He, Zhonghu,Wang, Aili,Yan, Yueming,He, Zhonghu,Bekes, Frank,Appels, Rudi. 2009

[3]Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars. Ma, Wujun,Liu, Wan,Gao, Xuan,Wang, Ke,Wang, Shunli,Yan, Yueming,Zhang, Yanzhen,Zhang, Yong,He, Zhonghu,Zhang, Yong,He, Zhonghu. 2012

[4]Development of introgression lines with 18 alleles of glutenin subunits and evaluation of the effects of various alleles on quality related traits in wheat (Triticum aestivum L.). Li, Yulian,Zhou, Ronghua,Jia, Jizeng,Branlard, Gerard,Li, Yulian. 2010

[5]QTL mapping for quantities of protein fractions in bread wheat (Triticum aestivum L.). He, Zhonghu,Yan, Jun,Zhang, Yong,Tang, Jianwei,Zhang, Yelun,Yan, Jun,Xiao, Yonggui,Zhang, Yan,Xia, Xianchun,He, Zhonghu.

[6]Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay. Sui, Xinxia,Wang, Linhai,Xia, Xianchun,He, Zhonghu,Sui, Xinxia,Wang, Zhenlin,He, Zhonghu. 2010

[7]Genetic Mapping of the Scab Resistance Gene in Cucumber. Zhang, Shengping,Miao, Han,Gu, Xing-fang,Yang, Yuhong,Xie, Bingyan,Wang, Xiaowu,Huang, Sanwen,Du, Yongchen,Sun, Rifei,Wehner, Todd C.. 2010

[8]Identification of microsatellite markers linked to powdery mildew resistance gene Pm2 in wheat. Qiu, Y. C.,Sun, X. L.,Zhou, R. H.,Kong, X. Y.,Zhang, S. S.,Jia, J. Z.. 2006

[9]Allelic variants of phytoene synthase 1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour colour. He, X. Y.,He, Z. H.,Xia, X. C.,He, Z. H.,Ma, W.,Appels, R.. 2009

[10]宁夏小麦Glu-A3与Glu-B3位点等位变异组成分析. 马丽,白升升,亢玲,王晓龙,张晓科,陈东升. 2018

[11]陕西小麦Glu-A3和Glu-B3位点等位变异的检测和分析. 梁强,王晓龙,张晓科,张晶,张同兴. 2011

[12]野生二粒小麦(Triticumdicoccoides)低分子量谷蛋白亚基基因的克隆与序列分析. 李巧云,安学丽,肖英华,张倩,张艳贞,夏先春,何中虎,晏月明. 2007

[13]小麦低分子量麦谷蛋白亚基及其编码基因研究进展. 赵献林,夏先春,刘丽,何中虎,孙其信. 2007

[14]野生二粒小麦(Triticum dicoccoides)低分子量谷蛋白亚基基因的克隆与序列分析. 李巧云,安学丽,肖英华,张倩,张艳贞,夏先春,何中虎,晏月明. 2007

[15]Functional analysis of polyphenol oxidase 1 gene in common wheat. Zhai, Shengnan,Liu, Hang,Xia, Xianchun,Li, Haosheng,Cao, Xinyou,He, Zhonghu,Ma, Wujun,Liu, Cheng,Song, Jianmin,Liu, Aifeng,Zhang, Jingjuan,Liu, Jianjun. 2023

[16]Unraveling crop enzymatic browning through integrated omics. Wang, Chunkai,Meng, Lin,Zhang, Guochao,Yang, Xiujun,Pang, Bingwen,Cheng, Junjie,He, Bing,Sun, Fushan. 2024

[17]水稻抗白叶枯病基因Xa23的PCR分子标记定位及辅助选择. 潘海军,王春连,赵开军,章琦,樊颖伦,周少川,朱立煌. 2003

[18]水稻抗白叶枯病基因Xa23的PCR分子标记定位及铺助选择. 潘海军,王春连,赵开军,章琦,樊颖论,周少川,朱立煌. 2003

[19]水稻抗白叶枯病基因Xa23群体的MAS育种研究. 于洁,王耀雯,马文清,王建岭,王育荣,赵开军,王春连,刘丕庆. 2010

[20]DNA分子标记在梨育种上的应用概况. 樊丽,方成泉,王斐,王志刚,董星光,林盛华. 2008

作者其他论文 更多>>