数字农科院2.0

Broad-Specificity Immunoassays for Detecting Prochloraz and Its Metabolites in Grapes and Water

文献类型： 外文期刊

作者： Zhao, Yun;Li, Peipei;Abd El-Aty, A. M.;Lei, Xingmei;Zhao, Jing;Xu, Lingyuan;Gao, Song;Li, Jia;She, Yongxin;Jin, Fen;Wang, Jing;Zheng, Lufei;Hammock, Bruce D.;Jin, Maojun

作者机构：

关键词： prochloraz and its metabolites;computational chemistryanalysis;broad-specificity antibody;recognitionmechanism;immunoassay

期刊名称： ACS AGRICULTURAL SCIENCE & TECHNOLOGY

ISSN：

年卷期： 2025 年

页码：

收录情况： ESCI（2025版）

摘要： This study aimed to develop broad-specificity antibodies for detecting prochloraz and its metabolites. Initially, three haptens were designed and identified through computational chemical methods, and the hapten H2 was the optimal candidate. Using H2-BSA as an immunogen, the monoclonal antibody 9C3 was prepared to recognize PCH and two metabolites, BTS44595 and BTS44596. The broad-specificity recognition mechanism revealed that the 2,4,6-trichlorophenyl moiety of the three targets is completely encapsulated in the binding pocket of mAb 9C3 and that hydrophobic forces play crucial roles in binding. Furthermore, an indirect enzyme-linked immunosorbent assay and a colloidal gold immunochromatographic assay for detecting prochloraz residues in grapes and river water samples were developed. The IC50 of ic-ELISA was 8.08-15.07 ng/mL, with recovery rates ranging from 73.80% to101.13%. In addition, the visual detection limit of GICA was 250-500 ng/mL, with IC50 values ranging from 98.04 to 126.29 ng/mL and CRs ranging from 67.2 to 78.02%. The accuracy and precision of both immunoassays were validated by LC-MS/MS.

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