数字农科院2.0

Identification and Functional Analysis of Odorant Binding Proteins in Apriona germari (Hope)

文献类型： 外文期刊

作者： Yuan, Tingting;Mang, Dingze;Purba, Endang R.;Ye, Jia;Qian, Jiali;Rao, Fuqiang;Wang, Haichao;Wu, Zhenchen;Zhang, Wenjing;Zheng, Yongxin;Zhang, Qing-He;Li, Zhaoqun;Zhang, Longwa

作者机构：

关键词： odorant binding protein (OBP);olfaction;ligandbinding assay;pheromone;plant volatile;insecticides

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2024 年

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Apriona germari (Hope) presents a significant threat as a dangerous wood-boring pest, inflicting substantial harm to forest trees. Investigating the olfactory sensory system of A. germari holds substantial theoretical promise for developing eco-friendly control strategies. To date, however, the olfactory perception mechanism in A. germari remains largely unknown. Therefore, we performed transcriptome sequencing of A. germari across four distinct body parts: antennae, foreleg tarsal segments, mouthparts (maxillary and labial palps), and abdomen terminals, pinpointing the odorant binding protein (OBP) genes and analyzing their expression. We found eight AgerOBPs (5, 19, 23, 25, 29, 59, 63, 70) highly expressed in the antennae. In our competitive binding experiments, AgerOBP23 showed strong binding abilities to the pheromone component fuscumol acetate, eight plant volatiles (farnesol, cis-3-hexenal, nerolidol, myristol acetate, cis-3-hexenyl benzoate, (-)-alpha-cedrene, 3-ethylacetophenone, and decane), and four insecticides (chlorpyrifos, phoxim, indoxacarb, and cypermethrin). However, AgerOBP29 and AgerOBP63 did not show prominent binding activities to these tested chemicals. Through homology modeling and molecular docking, we identified the key amino acid sites involved in the binding process of AgerOBP23 to these ligands, which shed light on the molecular interactions underlying its binding specificity. Our study suggests that AgerOBP23 may serve as a potential target for future investigations of AgerOBP ligand binding. This approach is consistent with the reverse chemical ecology principle, establishing the groundwork for future studies focusing on attractant or repellent development by exploring further the molecular interactions between OBP and various compounds.

分类号：

  • 相关文献

[1]The morphology and molecular mechanisms of enhanced olfaction in the grain aphid Sitobion miscanthi. Jia Fan,Xin Jiang,Qian Li,Miaomiao Yu,Siyu Zhang,Wenxin Xue,Frédéric Francis,Changqing Su,Gudbjorg Inga Aradottir,Yanxia Liu,Yong Zhang,Julian Chen. 2025

[2]Slow Release of Plant Volatiles Using Sol-Gel Dispensers. Bian, L.,Sun, X. L.,Chen, Z. M.,Cai, X. M..

[3]A Combination of Olfactory and Visual Cues Enhance the Behavioral Responses of Apolygus lucorum. Pan, Hongsheng,Xiu, Chunli,Lu, Yanhui,Pan, Hongsheng.

[4]Olfactory Response of Trichogramma ostriniae (Hymenoptera: Trichogrammatidae) to Volatiles Emitted by Mungbean Plants. Wang Zhen-ying,He Kang-lai,Dae-joon, Im. 2011

[5]An olfactory receptor from Apolygus lucorum (Meyer-Dur) mainly tuned to volatiles from flowering host plants. Zhang, Jin,Li, Guo-Qing,Yan, Shu-Wei,Zhang, Jin,Liu, Yang,Wang, Gui-Rong.

[6]The Salicylic Acid-Mediated Release of Plant Volatiles Affects the Host Choice of Bemisia tabaci. Chen, Gong,Tian, Lixia,Peng, Zhengke,Xie, Wen,Wu, Qingjun,Wang, Shaoli,Zhang, Youjun,Shi, Xiaobin,Zhou, Xuguo. 2016

[7](E)-beta-caryophyllene functions as a host location signal for the rice white-backed planthopper Sogatella furcifera. Wang, Qi,Xin, Zhaojun,Li, Jiancai,Hu, Lingfei,Lou, Yonggen,Lu, Jing,Wang, Qi,Xin, Zhaojun.

[8]A Female-Biased Odorant Receptor from Apolygus lucorum (Meyer-Dur) Tuned to Some Plant Odors. Zhang, Meiping,Zhang, Zhixiang,Yan, Shuwei,Wang, Guirong,Liu, Yang. 2016

[9]4-Ethylacetophenone from Potato Plants Repels Phthorimaea operculella and Inhibits Oviposition: A Sustainable Management Strategy. Xinyu Ma,Junjie Yan,Guangyuan Su,Fathiya M. Khamis,Athanase Hategekiman,Yulin Gao. 2025

[10]Distinct protocerebral neuropils associated with attractive and aversive female-produced odorants in the male moth brain. Jonas Hansen Kymre,XiaoLan Liu,Elena Ian,Christoffer Nerland Berge,GuiRong Wang,Bente Gunnveig Berg,XinCheng Zhao,Xi Chu. 2021

[11]Distinct protocerebral neuropils associated with attractive and aversive female-produced odorants in the male moth brain. Jonas Hansen Kymre,XiaoLan Liu,Elena Ian,Christoffer Nerland Berge,GuiRong Wang,Bente Gunnveig Berg,XinCheng Zhao,Xi Chu. 2022

[12]Synthetic Nasonov gland pheromone enhances abundance and visitation of honeybee, Apis mellifera, in Korla fragrant pear, Pyrus sinkiangensis. Li, Qian,Sun, Mengxiao,Liu, Yangtian,Liu, Bing,van Der Werf, Wopke,Bianchi, Felix J. J. A.,Lu, Yanhui. 2023

[13]A mosaic of endogenous and plant-derived courtship signals in moths. Yang Liu,Jeremy J. Heath,Sai Zhang,Michiel van Wijk,Guirong Wang,Jan Buellesbach,Ayako Wada-Katsumata,Astrid T. Groot,Coby Schal. 2023

[14]Identification, synthesis, and field evaluation of components of the female-produced sex pheromone of Helopeltis cinchonae (Hemiptera: Miridae), an emerging pest of tea. Magsi, Fida Hussain,Cai, Xiaoming,Luo, Zongxiu,Li, Zhaoqun,Bian, Lei,Xiu, Chunli,Fu, Nanxia,Li, Jianlong,Hall, David R.,Chen, Zongmao. 2024

[15]Functional analysis of the mating type genes in Verticillium dahliae. Ya Duo Zhang,Xiao Bin Ji,Juan Zong,Xiao Feng Dai,Steven J. Klosterman,Krishna V. Subbarao,Dan Dan Zhang,Jie Yin Chen. 2024

[16]Synthesis and bioactivity studies of the potato tuber moth, Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae) sex pheromone analogs. Yan, Junjie,Zhang, Mengdi,Li, Jing,Rondon, Silvia I.,Gao, Yulin. 2024

[17]Diversity and role of volatile terpene and terpenoid pheromones in insects. Yang, Jiu-Chun,Zhang, Jin-Ping,Wu, Chun-Yan,Bai, Yun,Guedes, Raul Narciso C.,Dewer, Youssef,Li, Feng-Qi,Zang, Lian-Sheng. 2025

[18]A pheromone from cuticular hydrocarbons regulates mating behavior in the hoverfly Eupeodes corollae. Wenbiao Liu, Chenxi Cai,Jinan Wu, Bing Wang. 2025

[19]Olfactory co-receptor identification and expression pattern analysis in polyembryonic endoparasitoid Macrocentrus cingulum. Ahmed, Tofael,Zhang, Tian-tao,Wang, Zhen-ying,He, Kang-lai,Bai, Shu-xiong,Ahmed, Tofael. 2015

[20]Proteomic analysis of castor bean tick Ixodes ricinus: a focus on chemosensory organs. Dani, Francesca Romana,Ban, Liping,Song, Limei,Pelosi, Paolo,Dani, Francesca Romana.

作者其他论文 更多>>