数字农科院2.0

Photoperiod regulation of diapause development in Loxostege sticticalis: coordinated 20-hydroxyecdysone dynamics and PTTH/EcR/USP

文献类型： 外文期刊

作者： Cui Jin;Liu YueQiu;Lv XiaoFei;Gao Yu;Zhang Lei;Xie DianJie;Cheng Yunxia;Jiang XingFu

关键词： Loxostege sticticalis; diapause regulation; 20-hydroxyecdysone; PTTH/EcR/USP; hormone agonist; RNA interferenc

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： BACKGROUND: The beet webworm, Loxostege sticticalis, a globally notorious agricultural pest enters diapause as mature larvae to overwinter in the northern temperate zone. The moth develops normally at 16L: 8D and 22 ± 1 °C, and enters diapause at 22 ± 1 °C and 12L:12D. While juvenile hormone (JH) regulates larval diapause in this insect species, the role of photoperiod- 20-hydroxyecdysone (20E) Axis remains enigmatic. RESULTS: Photoperiod-driven diapause progression is orchestrated through synchronized changes in 20E titers and expression of PTTH, EcR, and USP. Under short-day conditions, mature larvae exhibited 20E peaks followed by sustained lower levels (≈50 ng/mg) during diapause, PTTH expression was suppressed at diapause while EcR/USP co-downregulation in maintained diapause. Diapause terminating individuals showed pre-activation of PTTH/EcR/USP transcription before 20E resurgence. However, non-diapause larvae under long-day photoperiod, steadily rising 20E titer were mirrored by progressive PTTH/EcR/USP upregulation. Functional validation revealed that microinjection of 20E agonist--tebufenozide at early fifth-instar significantly prolonged larval development by 5.8 days but accelerated pupation by 11.9 days. Conversely, RNAi-mediated knockdown of PTTH, EcR, or USP dramatically shortened the fifth-instar duration but led to a significant 9.70 days extension of the mature larval phase. CONCLUSION: Our results establish a causal chain wherein photoperiod disrupts 20E biosynthesis via PTTH suppression, leading to EcR/USP silencing that sustains diapause. Pharmacological and genetic interventions demonstrate that the PTTH-20E-EcR/USP axis governs developmental transitions during both diapause and non-diapause trajectories.

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