数字农科院2.0

Characterization of Candida albicans myosin-1 motor function and its regulation by phosphorylation at TEDS site

文献类型： 外文期刊

作者： Xiao Xiao Zhu;Qin Tian;Ning Zhang;Qin Rong Tan;Guangfei Tang;Zhang Lei;Bing Chen;Xiang dong Li

作者机构：

关键词： Actin;Endocytosis;fungi;Myosin;Phosphorylation

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 307 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Class I myosin is a single-headed myosin, distributed from fungi to mammals. The pathogenic yeast Candida albicans expresses a solo class I myosin gene (CaMyo1). The yeast-to-hyphae transition of Candida albicans depends on CaMyo1 and phosphorylation at its TEDS site (S366). In this study, we characterized the motor activity of CaMyo1 using purified CaMyo1IQ2 (a truncated CaMyo1 containing the motor domain and two IQ motifs) recombinantly expressed in insect Sf9 cells. We found that S366 phosphorylation activated the actin-activated ATPase activity of CaMyo1IQ2 from ∼4 s−1 in the dephosphorylated state to ∼10 s−1 in the phosphorylated state. The ADP release rate of acto-CaMyo1IQ2 is >150 s−1 regardless of S366 phosphorylation, which is at least 10 times faster than the actin-activated ATPase rate (4–10 s−1), suggesting that CaMyo1 is a low-duty ratio motor. Interestingly, in the absence of actin, CaMyo1IQ2 has relatively high ATPase activity (∼ 0.7 s−1) with the ADP release (∼ 1 s−1) as the rate-limiting step, suggesting a substantial portion of cycling through futile actin-detached path. Both S366D and S366E mutants exhibit intermediate actin-activated ATPase activity between unphosphorylated and phosphorylated wild-type, demonstrating partial phosphomimetic functionality at residue S366.

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