数字农科院2.0

Enhancing gougerotin production by screening endogenous promoters for the transporter gene gouM in Streptomyces albulus CK-15

文献类型： 外文期刊

作者： Binghua Liu;Qianying Zhou;Ruixin Qiao;Kunping Zhou;Ning Zhang;Beibei Ge

作者机构：

关键词： endogenous promoter;gougerotin;high-yield strain;Streptomyces albulus;transporter gene

期刊名称： Frontiers in Microbiology

ISSN：

年卷期： 2026 年 16 卷

页码：

收录情况： SCIE（2025版）

摘要： Introduction: Gougerotin is a nucleoside antibiotic that exhibits strong inhibitory effects against bacteria, as well as activities against plant viruses and pathogenic fungi, making it highly valuable for development and application. However, its widespread use is limited by low production yield and long fermentation time. In the gougerotin biosynthetic gene cluster, the gouM gene is a transporter gene, and its encoded protein is responsible for transporting gougerotin to the extracellular space. Methods: To validate the impact of the gouM gene on gougerotin production, we generated a mutant strain by knocking out the gouM gene. To enhance the expression level of the gouM gene and thereby improve its ability to transport gougerotin, we screened endogenous promoters to drive the expression of the gouM gene through experiments including transcriptome sequencing, analysis, and measurement of mCherry fluorescence intensity. Results: The mutant strain S. albulus ΔgouM produced 508.34 mg/L of gougerotin, a 58.01% reduction compared to the wild-type strain (1212.74 mg/L). The results indicate that the transporter gene gouM significantly affects the yield of gougerotin. We screened seven suitable endogenous promoters (PT1, PT2, PT3, PM3, PM4, PL2, and PL3). Each of these promoters was then used to drive the expression of the gouM gene. Among them, the gougerotin yields of strains with gouM driven by PT1 and PT2 promoters were 1451.11 mg/L and 1474.58 mg/L, which represented increases of 19.65% and 21.59%, respectively, compared to the wild-type strain. Discussion: This study demonstrates that using strong promoters to enhance the expression level of the transporter gene gouM can increase the yield of gougerotin, thereby providing a basis for subsequently using promoter engineering strategies to construct higher-yielding strains in the future.

分类号：

  • 相关文献

[1]Efficacy of a high-yield attenuated vaccine strain wholly derived from avian influenza viruses by use of reverse genetics. Liu, Ming,Liu, Ming.

[2]Enhancing wuyiencin productivity of Streptomyces albulus (CK15) by mutagenesis breeding with atmospheric and room temperature plasma. Yu Wah Thein,Liming Shi,Binghua Liu,Qiuhe Wei,Kecheng Zhang,Beibei Ge. 2023

[3]Identification Of Wyspii As An A.ctivator Of Morphological Development I n Streptomyces Albulus Ck-15. Liu, BH,Ge, BB,Ma, JJ,Wei, QH,Khan, AA,Shi, LM,Zhang, KC. 2018

作者其他论文 更多>>