数字农科院2.0

Study on the mechanism of LNC004268 regulating sheep myoblast proliferation and differentiation through hnRNPK-CNOT2 Axis

文献类型： 外文期刊

作者： Yize Song;Yufang Liu;Runqing Chi;Peng Wang;Ran Di;Xiaoyun He;Shengguo Zhao;Mingxing Chu

作者机构：

关键词： CNOT2 gene;hnRNPK;lncRNA;mRNA stability;Myoblasts;Sheep

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 321 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The long non-coding RNAs (lncRNAs) can bind to transcription factors or RNA-binding proteins to play important regulatory roles in muscle growth and development. This study investigated the functional role of the LNC_004268 in sheep myoblast proliferation and differentiation, as well as its interaction with the RNA-binding protein hnRNPK and the downstream target gene CNOT2. LNC_004268 shows higher expression in the longissimus dorsi muscle of Small-tailed Han sheep (STH) compared to Sunite sheep (SNT) and is primarily localized in the nucleus. Functionally, LNC_004268 inhibits myoblast proliferation while promoting differentiation and myotube formation. Mechanistically, LNC_004268 binds hnRNPK, stabilizing CNOT2 mRNA. Actinomycin D (ACD) assays confirmed that LNC_004268 or hnRNPK overexpression delays CNOT2 mRNA decay, with combined expression further enhancing stability. Notably, CNOT2 functionally recapitulates LNC_004268's effects on myogenesis. Collectively, our study demonstrates that LNC_004268 regulates myoblast proliferation and differentiation by stabilizing CNOT2 mRNA via hnRNPK interaction, advancing understanding of lncRNA mediated muscle development regulation.

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