数字农科院2.0

MdPR4, a pathogenesis-related protein in apple, is involved in chitin recognition and resistance response to apple replant disease pathogens

文献类型： 外文期刊

作者： Zhe Zhou;Yanmin Zhu;Yi Tian;Jia-Long Yao;Shuxun Bian;Hengtao Zhang;Ruiping Zhang;Qiming Gao;Zhenli Yan

作者机构：

关键词： Apple root; Chitin; Fusarium spp.; MdPR4; Replant disease; Resistant response

期刊名称： J. Plant Physiol.

ISSN： 0176-1617

年卷期： 2021 年 260 卷

页码：

收录情况： JCR（2021版）

摘要： To maximize breeding and exploitation of disease resistance traits for managing apple replant disease (ARD), it is of great importance to understand the mechanisms of apple root resistance. Currently, little is known about the functions of the specific genes that confer resistance traits in apple root. In this study, molecular, biochemical, and genetic approaches allowed an in-depth understanding of the role of the MdPR4 gene in the defense response of apple root. The MdPR4 encoding gene showed upregulation following ARD pathogen inoculation in our previous transcriptome data. Subcellular localization analyses revealed that MdPR4 is localized on the plasma membrane, endoplasmic reticulum, and apoplast, which is mainly determined by its signal peptide. Molecular docking analysis between MdPR4 protein with chitin molecule and in vitro MdPR4 chitin affinity assay proved its chitin-binding ability, which provided evidence for its role in chitin-mediated immune responses. Purified MdPR4 protein and MdPR4 overexpressed apple callus inhibited spore germination and mycelial growth of ARD-related Fusarium spp. pathogens. These data support the conclusion that MdPR4 is a chitin-binding protein in apple vegetative tissues that may play an important role in defense activation in response to ARD pathogen infection. © 2021 Elsevier GmbH

分类号：

  • 相关文献

[1]The Expression and Sequence Analysis of MdMATE52 in Apple Roots During Activation of Defense Against Pythium ultimum Infection. Yanmin Zhu,Bianca Ortiz-Uriarte,Jordan Rainbow,Zhe Zhou. 2024

[2]Developmental Characteristics Of Grapevine Seedlings R.oot Border Cells And T heir Response To Rho-Hydroxybenzoic Acid. Guo, Xiuwu,Liu, Zhendong,Ma, Li,Li, Kun,Liu, Qianwen,Gun, Yinshan. 2019

[3]Nb-Lrrs Not Responding Consecutively To F.usarium Oxysporum Proliferation Caused R eplant Disease Formation Of Rehmannia Glutinosa. Chen, AG, Gu, L, Xu, N, Feng, FJ, Chen, DX, Yang, CY, Zhang, B, Li, MJ, Zhang, ZY. 2019

[4]Nematode Chitin And Application .. Peng, Deliang,Chen, Qi. 2019

[5]Rna-Seq Analysis Of Clitea Metallica (Coleoptera: Chrysomelidae), Provides Insights Into Cuticle-Related Genes And Mirnas. Lyu, Yue,Shang, Feng,Wang, Jin-Jun,Niu, Jinzhi,Xie, Xiu-Cheng,Ding, Bi-Yue,Xie, Xiu-Cheng,Lyu, Yue,Shang, Feng,Wang, Jin-Jun,Niu, Jinzhi,Ding, Bi-Yue. 2019

[6]Molecular Mechanism Of Bjchi1-Mediated Plant D.efense Against Botrytis Cinerea I nfection. Gao, Y, Zhao, KJ. 2017

[7]Transcriptomic Response Of Wolf Spider, P.ardosa Pseudoannulata, To Transgenic R ice Expressing Bacillus Thuringiensis Cry1Ab Protein. Wang, J, Peng, YD, Xiao, KF, Wei, BY, Hu, JL, Wang, Z, Song, QS, Zhou, XG. 2017

[8]An Optimized Cocktail Of Chitinolytic E.nzymes To Produce N,N ' -Diacetylchitobiose And N-Acetyl-D-Glucosamine From Defatted Krill By-Products. Chu, FM, Wang, D, Liu, T, Han, HY, Yu, YK, Yang, Q. 2019

[9]Aspergillus Flavus Gpi-Anchored Protein-Encoding Ecm33 H.as A Role In G rowth, Development, Aflatoxin Biosynthesis, And Maize Infection. Chang, PK, Zhang, Q, Scharfenstein, L, Mack, B, Yoshimi, A, Miyazawa, K, Abe, K. 2018

[10]Functional Characterization Of An Apple (.& It;Malus & It;X & It; Domestica & It;) Lysm Domain Receptor Encoding Gene For Its Role In Defense Response. Zhou, Z, Tian, Y, Cong, PH, Zhu, YM. 2018

[11]Potent Fungal Chitinase for the Bioconversion of Mycelial Waste. Liu, Tian,Liu, Tian,Yang, Qing,Yang, Qing,Han, Hongyu,Zhou, Yong,Han, Hongyu,Wang, Di,Guo, Xiaoguang,Fukamizo, Tamo,Yang, Qing. 2020

[12]Immune Responses Of Mammals And P.lants To Chitin-Containing Pathogens. Merzendorfer, Hans,Jiang, Xi,Bao, Han,Yang, Qing,Yang, Qing. 2019

[13]Biochemical Characterization Of A Beta-N-Acetylhexosaminidase F.rom Streptomyces Alfalfae And I ts Application In The Production Of N-Acetyl-D-Glucosamine. Lv, CY, Gu, TY, Xu, KY, Gu, JG, Li, LC, Liu, XN, Zhang, AD, Gao, SX, Li, WJ, Zhao, GG. 2019

作者其他论文 更多>>