数字农科院2.0

An ovalbumin fusion strategy to increase recombinant protein secretion in chicken eggs

文献类型： 外文期刊

作者： Long Xie;Zhenwen Huang;Meiyu Lan;Yaqi Cao;Lingling Sun;Lang Zhang;Erwei Zuo;Yangqing Lu

作者机构：

关键词： （1-2-2）Bioreactor;Deposited foreign protein in eggs;Genetically modified chicken;Nonsecretory protein secretion;Recombinant protein secretion

期刊名称： Journal of Biological Engineering

ISSN： 1754-1611

年卷期： 2024 年 18 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Maternal secretion of recombinant proteins into chicken eggs may provide a viable approach for pharmaceutical production but remains limited by poor secretion efficiency through the membrane of oviduct cells, despite high expression levels. Here, we used site-specific integration of an EGFP fused to the OVAL gene by a rigid linker, (EAAAK)3, at the endogenous ovalbumin locus in chicken primordial germ cells to generate OVAL-E3-EGFP transgenic chickens, with transgenic chickens expressing CMV immediate enhancer/β-actin-driven EGFP (CAG-EGFP) as a non-secreted control. In OVAL-E3-EGFP chickens, EGFP protein produced in maternal oviducts accumulates to high levels in eggs, but not in eggs of CAG-EGFP chickens. These results indicated that the secretion of foreign proteins can be substantially increased through fusion to the highly secreted endogenous ovalbumin. This study describes a basis for high yield recombinant protein expression in chicken eggs, enabling rapid and scalable production of numerous pharmaceutical proteins or metabolites.

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