数字农科院2.0

Suppression Of Hyaluronidase Reduces Invasion And Establishment Ofhaemonchus Contortuslarvae In Sheep

文献类型： 外文期刊

作者： Yang, XS; Khan, S; Zhao, XC; Zhang, JY; Nisar, A; Feng, XA

作者机构：

关键词： Haemonchus Contortus; Hyaluronidase; Rna Interference; Virulence Factor; Ex Vivo Tissue Explants; In Vivo

期刊名称： VETERINARY RESEARCH

ISSN： 0928-4249

年卷期： 2020 年 51 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Haemonchus contortusis a hematophagous endoparasite of small ruminants, which is responsible for huge economic losses in livestock sector. Hyaluronidase produced by infective larvae ofH. contortuscan degrade hyaluronic acid present in the host's abomasal tissue. Thus, it facilitates larval tissue invasion and early establishment. We herein explored this ability of hyaluronidase inH. contortus, and tested whether hyaluronidase is utilized as a virulence factor byH. contortuswhile establishing the infection. We first successfully blocked the hyaluronidase gene in L3 larvae by RNA interference (RNAi), which was subsequently confirmed by qPCR, enzymatic activity, and immunohistochemistry assays. Using these larvae we then conducted in vivo and in vitro assays on sheep to assess the effects of hyaluronidase suppression on larval invasion and establishment of infection. The in vivo assay showed a significant drop in worm burden in siRNA treated group in comparison to control group. During in vitro assay we applied an ovine ex vivo model where siRNA treated group of larvae showed significantly reduced invasion of the abomasal tissue explants as compared to control group. These findings indicate that hyaluronidase plays a key role in host's tissue invasion and larval establishment, and it is used as a virulence factor byH. contortuswhile establishing the infection. As an invasive virulence molecule, its functional research is thus conducive to the prevention of haemonchosis.

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