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HiBiT-tagged influenza A virus: A stable and efficient tool for antiviral reagent screening and vaccine evaluation

文献类型： 外文期刊

作者： Zhengxiang Wang;Wentao Shen;Xuegang Zhang;Yanli Wei;Yingying Du;Yingying Yu;Jing Wang;Qiyun Zhu;Qiaoying Zeng;Shuai Xu

作者机构：

关键词： antiviral screening;H9N2 IAV;luciferase tag;vaccine evaluation

期刊名称： Journal of Integrative Agriculture

ISSN： 2095-3119

年卷期： 2025 年 24 卷 10 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： Influenza A viruses (IAVs) possess variable pathogenic potency causing great economic losses in the poultry industry worldwide and threatening public health. The control of IAV epidemics desperately necessitates an efficient platform for screening antiviral compounds and evaluating vaccine efficacy. In this study, we utilized the H9N2 subtype IAV as the working model. An 11-amino-acid HiBiT tag, derived from NanoLuc luciferase, was incorporated into the flexible linker region of the NS1 protein. Subsequently, the recombinant HiBiT-tagged virus was rescued. The recombinant virus exhibited high genetic stability and similar virological characteristics to the parental virus, both in vitro and in vivo. Particularly importantly, the replication profile of the HiBiT-tagged virus can be easily measured using the Nano-Glo assay system, achieving an efficient screening platform. Based on this platform, we have developed assays with both convenience and efficiency for screening antiviral reagents, evaluating immunization efficacy, and measuring neutralizing antibodies.

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