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Molecular Characteristics And Adhesion Activity Of A Novel Protein ADP1 Of Arthrobotrys Oligospora To Nematodes

文献类型： 外文期刊

作者： Li Jie;Chen Shuangqing;Li Zhiyuan;Wang Lixia;Shang Yunxia;Gong Shasha;Xiao Chencheng;Zhang Kai;Zhang Xingxing;Cai Xuepeng;Qiao Jun;Meng Qingling

作者机构：

关键词： Adhesion protein;ADP1-nematode interactions;Arthrobotrys oligospora;Fusion protein

期刊名称： International Journal of Agriculture and Biology

ISSN： 1560-8530

年卷期： 2021 年 25.0 卷 6.0 期

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收录情况： 不在遴选数据库范围

摘要： Adhesion is a crucial step for nematode-trapping fungi (NTF) predating nematodes. To investigate the function of a novel protein ADP1 in nematode-trapping process, ADP1 gene of a representative NTF-Arthrobotrys oligospora was cloned and the molecular characteristics of this protein were analyzed. Then, the GFP chimeric ADP1 (ADP1-GFP) was generated in a GFP expression vector and expressed in Escherichia coli BL21 (DE3) and the recombinant ADP1-GFP (reADP1-GFP) was purified. Incubation of reADP1-GFP with J3 larvae of Caenorhabditis elegans and Haemonchus contortus showed that reADP1-GFP could adhere nematodes with the strongest adhesion ability at 25°C, while the reADP1-GFP treated by trypsin completely lost the adhesion ability. Furthermore, the numbers of captured nematodes of A. oligospora treated by antireADP1-GFP serum in the experimental group was significantly lower than that in the control group, which suggests that the nematode-trapping activity of A. oligospora is inhibited by anti-ADP1 serum. To the best of our knowledge, this is the first report confirming that ADP1 from A. oligospora can modulate the adherence to C. elegans and H. contortus. The exploration of interaction between ADP1 protein of A. oligospora and nematodes provides new insights into the process of invasion and molecular mechanisms of A. oligospora preying nematode.

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