数字农科院2.0

Selenium deficiency induces spleen pathological changes in pigs by decreasing selenoprotein expression, evoking oxidative stress, and activating inflammation and apoptosis

文献类型： 外文期刊

作者： Li Shuang;Li Shuang;Sun Wenjuan;Sun Wenjuan;Zhang Kai;Zhang Kai;Zhu Jiawei;Zhu Jiawei;Jia Xueting;Jia Xueting;Guo Xiaoqing;Guo Xiaoqing;Zhao Qingyu;Zhao Qingyu;Tang Chaohua;Tang Chaohua;Yin Jingdong;Yin Jingdong;Zhang Junmin;Zhang Junmin;Wang S.;Hu C.

作者机构：

关键词： Apoptosis; Inflammation; Oxidative stress; Pigs; Selenium deficiency; Spleen

期刊名称： Journal of Animal Science and Biotechnology

ISSN： 1674-9782

年卷期： 2021 年 12 卷 1 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 科技核心（2020版）

摘要： Background: The immune system is one aspect of health that is affected by dietary selenium (Se) levels and selenoprotein expression. Spleen is an important immune organ of the body, which is directly involved in cellular immunity. However, there are limited reports on Se levels and spleen health. Therefore, this study established a Se-deficient pig model to investigate the mechanism of Se deficiency-induced splenic pathogenesis. Methods: Twenty-four pure line castrated male Yorkshire pigs (45 days old, 12.50 ± 1.32 kg, 12 full-sibling pairs) were divided into two equal groups and fed Se-deficient diet (0.007 mg Se/kg) or Se-adequate diet (0.3 mg Se/kg) for 16 weeks. At the end of the trial, blood and spleen were collected to assay for erythroid parameters, the osmotic fragility of erythrocytes, the spleen index, histology, terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining, Se concentrations, the selenogenome, redox status, and signaling related inflammation and apoptosis. Results: Dietary Se deficiency decreased the erythroid parameters and increased the number of osmotically fragile erythrocytes (P < 0.05). The spleen index did not change, but hematoxylin and eosin and TUNEL staining indicated that the white pulp decreased, the red pulp increased, and splenocyte apoptosis occurred in the Se deficient group. Se deficiency decreased the Se concentration and selenoprotein expression in the spleen (P < 0.05), blocked the glutathione and thioredoxin antioxidant systems, and led to redox imbalance. Se deficiency activated the NF-κB and HIF-1α transcription factors, thus increasing pro-inflammatory cytokines (IL-1β, IL-6, IL-8, IL-17, and TNF-α), decreasing anti-inflammatory cytokines (IL-10, IL-13, and TGF-β) and increasing expression of the downstream genes COX-2 and iNOS (P < 0.05), which in turn induced inflammation. In addition, Se-deficiency induced apoptosis through the mitochondrial pathway, upregulated apoptotic genes (Caspase3, Caspase8, and Bak), and downregulated antiapoptotic genes (Bcl-2) (P < 0.05) at the mRNA level, thus verifying the results of TUNEL staining. Conclusions: These results indicated that Se deficiency induces spleen injury through the regulation of selenoproteins, oxidative stress, inflammation and apoptosis. © 2021, The Author(s).

分类号：

  • 相关文献

[1]Selenium deficiency induces spleen pathological changes in pigs by decreasing selenoprotein expression, evoking oxidative stress, and activating inflammation and apoptosis. Li Shuang,Li Shuang,Sun Wenjuan,Sun Wenjuan,Zhang Kai,Zhang Kai,Zhu Jiawei,Zhu Jiawei,Jia Xueting,Jia Xueting,Guo Xiaoqing,Guo Xiaoqing,Zhao Qingyu,Zhao Qingyu,Tang Chaohua,Tang Chaohua,Yin Jingdong,Yin Jingdong,Zhang Junmin,Zhang Junmin,Wang S.,Hu C.. 2021

[2]Selenium Deficiency-Induced Pancreatic Pathology Is Associated with Oxidative Stress and Energy Metabolism Disequilibrium. Shuang Li,Qingyu Zhao,Kai Zhang,Wenjuan Sun,Jing Li,Xiaoqing Guo,Jingdong Yin,Junmin Zhang,Chaohua Tang. 2021

[3]Research progress on oxidative stress and its nutritional regulation strategies in pigs. Hao Yue,Xing Mingjie,Gu Xianhong. 2021

[4]The Co-Expression Of Circrna And M.rna In The Thymuses O f Chickens Exposed To Ammonia. Chen, DC, Miao, ZY, Peng, MQ, Xing, HJ, Zhang, HF, Teng, XH. 2019

[5]Kidney Protection Effect Of Ginsenoside R.e And Its Underlying M echanisms On Cisplatin-Induced Kidney Injury. Wang, Z, Li, YF, Han, XY, Sun, YS, Zhang, LX, Liu, W, Liu, XX, Li, W, Liu, YY. 2018

[6]Application Of Transcriptome Analysis: Oxidative Stress, Inflammation And Microtubule Activity Disorder Caused By Ammonia Exposure May Be The Primary Factors Of Intestinal Microvilli Deficiency In Chicken. Wang, SC, Li, XJ, Wang, W, Zhang, HF, Xu, SW. 2019

[7]Rice-Derived Peptide Aagalps Inhibits Tnf-Alpha-Induced Inflammation And Oxidative Stress In Vascular Endothelial Cells. Tong, LT, Ju, ZY, Liu, LY, Wang, LL, Zhou, XR, Xiao, TZ, Zhou, SM. 2020

[8]Lactobacillus Brevis 23017 Relieves Mercury T.oxicity In The Colon B y Modulation Of Oxidative Stress And Inflammation Through The Interplay Of Mapk And Nf-Kappa B Signaling Cascades. Jiang, XP, Gu, SS, Liu, D, Zhao, LL, Xia, S, He, XM, Chen, HY, Ge, JW. 2018

[9]Phenethyl isothiocyanate ameliorates liver injuries secondary to inflammatory bowel disease. Ma, Congcong,Zhang, Li,Huang, Qingde,Deng, Qianchun,Huang, Fenghong,Xu, Jiqu. 2025

[10]Mogroside V ameliorates the oxidative stress-induced meiotic defects in porcine oocytes in vitro. Pan C., Chen J., Chen Y., Lu Y., Liang X., Xiong B., Lu Y.. 2022

[11]Dynamic Chromatin States Coupling with Key Transcription Factors in Colitis-Associated Colorectal Cancer. Chen L., Luo Z., Zhao C., Li Q., Geng Y., Xiao Y., Chen M.-K., Li L., Chen Z.-X., Wu M.. 2022

[12]Stabilized fermentation product of Cetobacterium somerae improves gut and liver health and antiviral immunity of zebrafish. Xie M., Xie Y., Li Y., Zhou W., Zhang Z., Yang Y., Olsen R.E., Ringø E., Ran C., Zhou Z.. 2022

[13]Splenic Microrna Expression Profiles And I.ntegration Analyses Involved In H ost Responses To Salmonella Enteritidis Infection In Chickens. Li, P, Fan, WL, Li, QH, Wang, J, Liu, RR, Everaert, N, Liu, J, Zhang, YH, Zheng, MQ, Cui, HX, Zhao, GP, Wen, J. 2017

[14]Boron Affects Immune Function Through M.odulation Of Splenic T L ymphocyte Subsets, Cytokine Secretion, And Lymphocyte Proliferation And Apoptosis In Rats. Jin, EH, Li, SH, Ren, M, Hu, QQ, Gu, YF, Li, K. 2017

[15]Characterization of a single-domain von Willebrand factor type C protein (HaSVC) from the salivary gland of the tick Hyalomma asiaticum. Gong H., Yao J., Zhang B., Zhou Y., Zhang H., Cao J., Wei N., Zhou J.. 2022

[16]Proteomic Profiling Of The Liver, Hepatic Lymph Nodes, And Spleen Of Buffaloes Infected With Fasciola Gigantica. Hu, RS, Zhang, FK, Elsheikha, HM, Ma, QN, Ehsan, M, Zhao, Q, Zhu, XQ. 2020

[17]Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice. 范沛,,韩宾,,胡菡,,魏俏红,,张旭凤,,孟丽峰,,唐晓峰,,王丽萍,,李建科. 2020

[18]Profiling Of The Perturbed Metabolomic S.tate Of Mouse Spleen D uring Acute And Chronic Toxoplasmosis. Chen, XQ, Zhou, CX, Elsheikha, HM, He, S, Hu, GX, Zhu, XQ. 2017

[19]Metagenomic Analysis Of Cecal Microbiome I.dentified Microbiota And Functional C apacities Associated With Feed Efficiency In Landrace Finishing Pigs. Chen, Shaokang,Yang, Ting,Wang, Yuan,Zhao, Xitong,Xing, Kai,Wang, Chuduan,Liu, Jianfeng,Ao, Hong,Zhang, Fengxia,Tan, Zhen. 2017

[20]Effects Of Graded Levels Of A.n Escherichia Coli Phytase O n Growth Performance, Apparent Total Tract Digestibility Of Phosphorus, And On Bone Parameters Of Weanling Pigs Fed Phosphorus-Deficient Corn-Soybean Meal. She, Y, Liu, YH, Gonzalez-Vega, JC, Stein, HH. 2017

作者其他论文 更多>>