数字农科院2.0

Genome-wide identification of Shaker K+ channel family in Nicotiana tabacum and functional analysis of NtSKOR1B in response to salt stress

文献类型： 外文期刊

作者： Yuan, Guang;Nong, Tongjia;Hunpatin, Oluwaseyi Setonji;Shi, Chuhan;Su, Xiaoqing;Xu, Fangzheng;Wang, Yihui;Zhang, Zhaoting;Ning, Yang;Liu, Haobao;Wang, Qian

作者机构：

关键词： tobacco;Shaker K plus channel;SKOR1B;salt stress;K plus /Na plus ratio

期刊名称： FRONTIERS IN PLANT SCIENCE

ISSN： 1664-462X

年卷期： 2024 年 15 卷

页码：

收录情况： SCIE（2024版）

摘要： Soil salinization poses a mounting global ecological and environmental threat. The identification of genes responsible for negative regulation of salt tolerance and their utilization in crop improvement through gene editing technologies emerges as a swift strategy for the effective utilization of saline-alkali lands. One efficient mechanism of plant salt tolerance is maintaining the proper intracellular K+/Na+ ratio. The Shaker K+ channels play a crucial role in potassium absorption, transport, and intracellular potassium homeostasis in plant cells. Here, the study presents the first genome-wide identification of Shaker K+ channels in Nicotiana tabacum L., along with a detailed bioinformatic analysis of the 20 identified members. Transcriptome analysis revealed a significant up-regulation of NtSKOR1B, an outwardly-rectifying member predominantly expressed in the root tissue of tobacco seedlings, in response to salt stress. This finding was then confirmed by GUS staining of ProNtSKOR1B::GUS transgenic lines and RT-qPCR analysis. Subsequently, NtSKOR1B knockout mutants (ntskor1) were then generated and subjected to salt conditions. It was found that ntskor1 mutants exhibit enhanced salt tolerance, characterized by increased biomass, higher K+ content and elevated K+/Na+ ratios in both leaf and root tissues, compared to wild-type plants. These results indicate that NtSKOR1B knockout inhibits K+ efflux in root and leaf tissues of tobacco seedlings under salt stress, thereby maintaining higher K+/Na+ ratios within the cells. Thus, our study identifies NtSKOR1B as a negative regulator of salt tolerance in tobacco seedlings.

分类号：

  • 相关文献

[1]烟草根际解钾茵的筛选与鉴定. 刘璇,孔凡玉,张成省,王静,冯超,赵杰. 2012

[2]Overexpression of NtCBL5A Leads to Necrotic Lesions by Enhancing Na+ Sensitivity of Tobacco Leaves Under Salt Stress. Jingjing Mao,Jiaping Yuan,Zhijie Mo,Lulu An,Sujuan Shi,Richard G.F. Visser,Yuling Bai,Yuhe Sun,Guanshan Liu,Haobao Liu,Qian Wang,C. Gerard van der Linden. 2021

[3]Overexpression of NtCBL5A Leads to Necrotic Lesions by Enhancing Na+ Sensitivity of Tobacco Leaves Under Salt Stress. Jingjing Mao,Jiaping Yuan,Zhijie Mo,Lulu An,Sujuan Shi,Richard G.F. Visser,Yuling Bai,Yuhe Sun,Guanshan Liu,Haobao Liu,Qian Wang,C. Gerard van der Linden. 2021

[4]Genome-wide identification of CBL family genes in Nicotiana tabacum and the functional analysis of NtCBL4A-1 under salt stress. Jingjing Mao,Guang Yuan,Kaiyan Han,Haiying Xiang,Wanli Zeng,Richard G.F. Visser,Yuling Bai,C. Gerard van der Linden,Haobao Liu,Qian Wang. 2023

[5]Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. ZhongQun, He,ChaoXing, He,ZhiBin, Zhang,ZhiRong, Zou,HuaiSong, Wang.

[6]MOLECULAR CLONING AND ANALYSIS OF A CONSTANS HOMOLOG FROM NICOTIANA TABACUM. Lu, Y.,Liu, Y.,Sun, Y.,Mu, J.,Ren, M.,Zhang, X.,Wang, Z.. 2013

[7]Analysis of global gene expression profiles in tobacco roots under drought stress. Pan, Guangtang,Yin, Fuqiang,Liu, Ming,Zhang, Wenyou,Qin, Cheng,Shen, Yaou,Lin, Haijian,Zhang, Zhiming,Yang, Aiguo,Luo, Chenggang,Liu, Haobao,Gao, Jian,Gao, Jian,Gao, Jian. 2015

[8]Expressing an (E)-beta-farnesene synthase in the chloroplast of tobacco affects the preference of green peach aphid and its parasitoid. Wang, Gen-Ping,Wang, Cheng-She,Xia, Lan-Qin,Wang, Gen-Ping,Yu, Xiu-Dao,Yu, Xiu-Dao,Fan, Jia. 2015

[9]Effects of intercropping vines with tobacco and root extracts of tobacco on grape phylloxera, Daktulosphaira vitifoliae Fitch. Su Jun-ping,Liu Wei-wei,Guo Yu-yuan. 2015

[10]The detection of QTLs controlling bacterial wilt resistance in tobacco (N-tabacum L.). Qian, Yi-liang,Yao, Da-nian,Qian, Yi-liang,Zu, Chao-long,Gao, Zheng-liang,Sun, Xue-yong,Wang, Xin-sheng,Wang, Da-zhou,Zhang, Hong-jun,Zhang, Hong-jun,Wang, Zhi-yong. 2013

[11]Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum). Ding, Anming,Marowa, Prince,Kong, Yingzhen.

[12]Co-efficacy of a Trap Crop, Colocasia esculenta (L.) Schott and a Biological Agent, Spodoptera litura Nuclear Polyhedral Virus on the Tobacco Caterpillar, Spodoptera litura (Fabricius) in the Tobacco Field. Zhou, Zhong-Shi,Xu, Zai-Fu,Zhou, Zhong-Shi,Chen, Ze-Peng. 2011

[13]NtPHYB1(K326), a homologous gene of Arabidopsis PHYB, positively regulates the content of phenolic compounds in tobacco. Zhao, Jiehong,Zhang, Jie,Li, Zhenhua,Yu, Jing,Yu, Shizhou,Guo, Yushuang,Fu, Yongfu,Zhang, Xiaomei,Han, Jie.

[14]Multiresidue determination of 114 multiclass pesticides in flue-cured tobacco by solid-phase extraction coupled with gas chromatography and tandem mass spectrometry. Cao, Jianmin,Sun, Na,Yu, Weisong,Pang, Xueli,Lin, Yingnan,Kong, Fanyu,Qiu, Jun.

[15]A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.). Wang, Di,Chen, Xiaobo,Song, Gaoyuan,Kong, Xingchen,Geng, Shuaifeng,Yang, Jiayue,Wang, Bingnan,Wu, Liang,Li, Aili,Mao, Long,Zhang, Zenglin,Liu, Danmei.

[16]Homology-based analysis of the GRAS gene family in tobacco. Chen, Y. Q.,Wang, D. W.,Ding, A. M.,Sun, T. T.,Wang, W. F.,Sun, Y. H.,Chen, Y. Q.,Sun, T. T.,Tai, S. S.. 2015

[17]Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion. Li, X,Yang, SH,Yu, XC,Jin, ZX,Li, WD,Li, L,Li, J,Li, MG.

[18]Genome-wide analysis of TCP family in tobacco. Chen, L.,Ding, A. M.,Chen, H.,Xia, F.,Wang, W. F.,Sun, Y. H.,Chen, L.,Chen, H.,Xia, F.,Wang, W. F.,Chen, Y. Q.. 2016

[19]Iprodione Residues and Dissipation Rates in Tobacco Leaves and Soil. Wang, Xiuguo,Xu, Guangjun,Wang, Fenglong,Sun, Huiqing,Li, Yiqiang.

[20]Genome-wide analysis of Water-stress-responsive microRNA expression profile in tobacco roots. Yin, Fuqiang,Gao, Jian,Qin, Cheng,Zhang, Zhiming,Shen, Yaou,Lin, Haijian,Pan, Guangtang,Yin, Fuqiang,Liu, Ming,Zhang, Wenyou,Xia, Mingzhong,Qin, Cheng,Yang, Aiguo,Luo, Chenggang.

作者其他论文 更多>>