数字农科院2.0

SNAC-Tag Integration into NPN Platform Facilitates Efficient Purification of Recombinant Proteins from Nicotiana benthamiana

文献类型： 外文期刊

作者： Hui-Xin Meng1;†; Hai-Ping Diao1;†; Jin-Hao Sun2; Shi-Yu Ren1; Xue-Jiao Xu1; Yong-Feng Guo1; Shi-Jian Song1;2; *

关键词： Plant molecular farming; Nicotiana benthamiana; SNAC-tag; Protein purification; Trimeric Proteins.

期刊名称： PLANT BIOTECHNOLOGY JOURNAL

ISSN： 1467-7644

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： We incorporated the sequence-specific nickel-assisted cleavage (SNAC)-tag into a natural peptidoglycan nanoparticle (NPN)-based purification platform. This approach employs NPN to efficiently enrich target proteins from plant total protein extracts, followed by Ni²⁺-mediated chemical cleavage to cost-effective and precisely remove the C-terminal NPN-binding domain, thereby releasing the target proteins

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