Primary evaluation of immune responses in mice after a single mRNA immunization with Echinococcus granulosus Eg95 and Coenurus cerebralis TM16
文献类型: 外文期刊
作者: Chun Ni Meng;Ting Ting Li;Wen Hui Li;Meng Wang;Wei Gang Chen;Hong Bin Yan;Li Li;Xiao Lin Sun;Bao Quan Fu;Nian Zhang Zhang
作者机构:
关键词: Eg95 antigen;Immune response;mRNA-LNP;Recombinant protein vaccine;TM16 antigen
期刊名称: Vaccine
ISSN: 0264-410X
年卷期: 2026 年 75 卷
页码:
收录情况: SCIE(2025版)
摘要: Echinococcus granulosus and Taenia multiceps cause zoonotic cystic diseases. Studies have shown that Eg95 from E. granulosus is highly conserved, exhibits excellent immunogenicity, and serves as a critical candidate antigen. In recent years, the TM16 recombinant protein antigen has been one of the most studied antigens for preventing C. cerebralis and demonstrates optimal experimental immunogenicity. This study developed both recombinant protein and mRNA-LNP vaccines targeting the Eg95 and TM16 antigens. Immunization of BALB/c mice showed that both vaccines significantly increased specific antibody levels (IgG, IgG1, IgG2a) and cytokines (IFN-γ, IL-4) (P < 0.001), inducing a predominant Th1-type immune response. The mRNA-LNP vaccine demonstrated superior efficacy in enhancing IgG2a levels and CD8+ T cell responses compared to the protein vaccine. These results indicate strong immunogenicity and support further evaluation of the Eg95-TM16 mRNA-LNP vaccine in intermediate hosts like sheep and goats.
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