数字农科院2.0

Metabolomics and Lipidomics Reveal the Metabolic Disorders Induced by Single and Combined Exposure of Fusarium Mycotoxins in IEC-6 Cells

文献类型: 外文期刊

作者: Xinlu Wang;Yanyang Xu;Haiqi Yu;Yushun Lu;Yongzhong Qian;Meng Wang

作者机构:

关键词: combined toxicity;fusarium mycotoxins;IEC-6 cells;lipidomics;metabolomics

期刊名称: Foods

ISSN: 2304-8158

年卷期: 2025 年 14 卷 2 期

页码:

收录情况: SCIE(2025版)

摘要: Deoxynivalenol (DON), fumonisin B1 (FB1), and zearalenone (ZEN) are typical fusarium mycotoxins that occur worldwide in foodstuffs, posing significant health hazards to humans and animals. Single and combined exposure of DON, FB1, and ZEN leads to intestinal toxicity but the toxicology mechanism research is still limited. In this study, we explored the cytotoxicity effects of DON, FB1, ZEN, and their combination in rat intestinal epithelial cell line 6 (IEC-6) cells. Cell viability results showed that the cytotoxicity potency ranking was DON > ZEN > FB1. Furthermore, both DON + FB1 and DON + ZEN presented synergism to antagonism effects based on a combination index (CI)-isobologram equation model. Integrated metabolomics and lipidomics was adopted to explore cell metabolism disorders induced by fusarium mycotoxin exposure. A total of 2011 metabolites and 670 lipids were identified. An overlap of 37 and 62 differential compounds was confirmed after single and combined mycotoxin exposure by multivariate analysis, respectively. Some of the differential compounds were endocellular antioxidants and were significantly downregulated in mycotoxin exposure groups, indicating metabolic disorders as well as antioxidant capacity damage in cells. Pathway enrichment analysis annotated ethanol metabolism production of ROS by CYP2E1 was mainly involved in the disturbance of DON, FB1, and ZEN. The results obtained in this study help to define the toxicity effects of DON, FB1, and ZEN singly and in co-existence, providing an important scientific basis for combined risk recognition of mycotoxin contamination.

分类号:

  • 相关文献

[1]Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows. Tian, He,Zheng, Nan,Li, Songli,Zhang, Yangdong,Wang, Jiaqi,Wang, Weiyu,Cheng, Jianbo.

[2]Integrated metabolomics and lipidomics evaluate the alterations of flavor precursors in chicken breast muscle with white striping symptom. Kong F.,Bai L.,He Z.,Sun J.,Tan X.,Zhao D.,Feng F.,Liu D.,Zhao G.,Wen J.,Liu R.. 2023

[3]Integrated non-targeted lipidomics and metabolomics analyses for fluctuations of neonicotinoids imidacloprid and acetamiprid on Neuro-2a cells. Xinlu Wang,Jing Qiu,Yanyang Xu,Guangqin Liao,Qi Jia,Yecan Pan,Tiancai Wang,Yongzhong Qian. 2021

[4]Characterization and difference of lipids and metabolites from Jianhe White Xiang and Large White pork by high-performance liquid chromatography–tandem mass spectrometry. Run Zhang,Man Yang,Xinhua Hou,Renda Hou,Ligang Wang,Lijun Shi,Fuping Zhao,Xin Liu,Qingshi Meng,Lixian Wang,Longchao Zhang. 2022

[5]Comparative characterization of flavor precursors and volatiles of Taihe black-boned silky fowl and Hy-line Brown yolks using multiomics and GC-O-MS-based volatilomics. Weifang Yang,Youyou Yang,Liang Wang,Xueze Lv,Jing Li,Huanxian Cui,Chaohua Tang,Qingyu Zhao,Yaxiong Jia,Yuchang Qin,Junmin Zhang. 2023

[6]Toxic effects of fipronil and its metabolites on PC12 cell metabolism. Xiao Song,Xinlu Wang,Guangqin Liao,Yecan Pan,Yongzhong Qian,Jing Qiu. 2021

[7]Integrated Metabolomics and Lipidomics Analysis Reveals Lipid Metabolic Disorder in NCM460 Cells Caused by Aflatoxin B1 and Aflatoxin M1 Alone and in Combination. Xue Yang,Xue Li,Yanan Gao,Jiaqi Wang,Nan Zheng. 2023

[8]Multi-omics analysis of a drug-induced model of bipolar disorder in zebrafish. Yameng Li,Lin Zhang,Mingcai Mao,Linjuan He,Tiancai Wang,Yecan Pan,Xiaoyu Zhao,Zishu Li,Xiyan Mu,Yongzhong Qian,Jing Qiu. 2023

[9]Seasonal variation in non-volatile flavor substances of fresh tea leaves (Camellia sinensis) by integrated lipidomics and metabolomics using UHPLC-Q-Exactive mass spectrometry. Chen, Le,Zhang, Shan,Feng, Yuning,Jiang, Yongwen,Yuan, Haibo,Shan, Xujiang,Zhang, Qianting,Niu, Linchi,Wang, Shengnan,Zhou, Qinghua,Li, Jia. 2024

[10]Carbendazim led to neurological abnormalities by interfering metabolic profiles in zebrafish brain after short-term exposure. Wang, Tiancai,Wang, Zishuang,Liao, Guangqin,Li, Xiabing,Gu, Jingyi,Qiu, Jing,Qian, Yongzhong. 2025

[11]Proteomic, metabolomic and lipidomic profiles provide insights on milk quality differences during heat treatment. Meiqing Chen,Ran Xiao,Shuang Du,Ashikur Rahman,Nan Zheng,Yangdong Zhang,Tianjiao Niu,Jiaqi Wang. 2025

[12]Comparison Of Concentration And Health R.isks Of 9 Fusarium M ycotoxins In Commercial Whole Wheat Flour And Refined Wheat Flour By Multi-Iac-Hplc. Zhang, YY, Pei, F, Fang, Y, Li, P, Zhao, Y, Shen, F, Zou, YY, Hu, QH. 2019

[13]Natural Occurrence of Conventional and Emerging Fusarium Mycotoxins in Freshly Harvested Wheat Samples in Xinjiang, China. Weihua Zheng,Jinyi Zhang,Yi Shi,Can He,Xiaolong Zhou,Junxi Jiang,Gang Wang,Jingbo Zhang,Jianhong Xu,Jianrong Shi,Fei Dong,Tao Sun. 2025

[14]Extended biotic ligand model for predicting combined Cu-Zn toxicity to wheat (Triticum aestivum L.): Incorporating the effects of concentration ratio, major cations and pH. Wang, Xuedong,Ji, Dongxue,Chen, Xiaolin,Li, Xiaoxiu,Ma, Yibing,Ma, Yibing,Yang, Junxing.

[15]Joint acute toxicity of the herbicide butachlor and three insecticides to the terrestrial earthworm, Eisenia fetida. Wang, Yanhua,Cang, Tao,Yu, Ruixian,Wu, Shenggan,Liu, Xinju,Wang, Qiang,Cai, Leiming,Chen, Chen.

[16]Assessing joint toxicity of four organophosphate and carbamate insecticides in common carp (Cyprinus carpio) using acetylcholinesterase activity as an endpoint. Wang, Yanhua,Zhao, Xueping,Wang, Qiang,Chen, Chen,Qian, Yongzhong.

[17]The combined toxicity assessment of carp (Cyprinus carpio) acetylcholinesterase activity by binary mixtures of chlorpyrifos and four other insecticides. Chen, Chen,Qian, Yongzhong,Wang, Yanhua,Zhao, Xueping,Wang, Qiang. 2014

[18]Extended Biotic Ligand Model For P.redicting Combined Cu-Zn Toxicity T o Wheat (Triticum Aestivum L.): Incorporating The Effects Of Concentration Ratio, Major Cations And Ph. Wang, XD, Ji, DX, Chen, XL, Ma, YB, Yang, JX, Ma, JX, Li, XX. 2017

[19]Joint Acute And Endocrine Disruptive T.oxicities Of Malathion, Cypermethrin A nd Prochloraz To Embryo-Larval Zebrafish, Darzio Rerio. Guo, DM, Wang, YH, Qian, YZ, Chen, C, Jiao, BN, Cai, LM, Wang, Q. 2017

[20]Variations of enzymatic activity and gene expression in zebrafish (Danio rerio) embryos co-exposed to zearalenone and fumonisin B1. Guiling Yang,Yanhua Wang,Tiancai Wang,Dou Wang,Hongbiao Weng,Qiang Wang,Chen Chen. 2021

作者其他论文 更多>>