数字农科院2.0

The role of OR5, which is highly expressed in the winged grain aphid Sitobion miscanthi, in specific recognition of EBF

文献类型： 外文期刊

作者： Jia Fan;Bing Wang;Tianjiao Zhang;Miaomiao Yu;Mengyu Wang;Siyu Zhang;Changqing Su;Jingyang Xu;Xin Jiang;Qian Zhang;Guirong Wang;Julian Chen

作者机构：

关键词： Alarm pheromone;Olfactory receptor;RNAi;Wing phenotype;Xenopus oocyte expression system

期刊名称： Insect Biochemistry and Molecular Biology

ISSN： 0965-1748

年卷期： 2024 年 173 卷

页码：

收录情况： SCIE（2024版）

摘要： Winged parthenogenetic aphids are mainly responsible for migration and dispersal. Aphid alarm pheromone (E)-β-Farnesene (EBF) has dual effects on repelling and stimulating wing differentiation in aphids. Previous studies have shown that the odorant coreceptor SmisOrco is involved in the perception of EBF by S. miscanthi; however, its EBF-specific odorant receptor (OR) and the difference between winged and wingless aphids remain unclear. In this study, the Xenopus oocyte expression system and RNAi technology were used to detect the transmission of EBF signals, and it was found that the olfactory receptor SmisOR5 is an EBF-specific OR in S. miscanthi and is specifically highly expressed in the antennae of winged aphids. Furthermore, when OR5 was silenced with dsRNA, the repellent effect of EBF was weakened, and aphids showed more active aimless movements. Therefore, as a specific OR for EBF, the high expression level of SmisOR5 in winged aphids suggests a molecular basis for its high sensitivity to EBF. This study advances our understanding of the molecular mechanisms of aphid EBF perception and provides novel ideas for effective management and prevention of the migration of winged aphids.

分类号：

  • 相关文献

[1]A Detailed Spatial Expression Analysis of Wing Phenotypes Reveals Novel Patterns of Odorant Binding Proteins in the Soybean Aphid, Aphis glycines. Ling Wang;Hang Yin,Zhiguo Zhu,Shuai Yang,Jia Fan. 2021

[2]The morphology and molecular mechanisms of enhanced olfaction in the grain aphid Sitobion miscanthi. Jia Fan,Xin Jiang,Qian Li,Miaomiao Yu,Siyu Zhang,Wenxin Xue,Frédéric Francis,Changqing Su,Gudbjorg Inga Aradottir,Yanxia Liu,Yong Zhang,Julian Chen. 2025

[3]Molecular basis of (E)-β-farnesene-mediated aphid location in the predator Eupeodes corollae. Bing Wang,Wanying Dong,Huimin Li,Chiara D'Onofrio,Penghua Bai,Ruipeng Chen,Lulu Yang,Jinan Wu,Xiaoqing Wang,Bo Wang,Dong Ai,Wolfgang Knoll,Paolo Pelosi,Guirong Wang. 2022

[4]Expressional and functional comparisons of five clustered odorant binding proteins in the brown marmorated stink bug Halyomorpha halys. Zehua Wang,Fan Yang,Ang Sun,Jingyi Song,Shuang Shan,Yongjun Zhang,Shanning Wang. 2022

[5]Identification Of An Intraspecific Alarm P.heromone and Two Conserved Odorant-Binding Proteins Associated With (E)-Beta-Farnesene Perception In Aphid Rhopalosiphum Padi. Fan, J,Xue, WX,Duan, HX,Jiang, X,Zhang, Y,Yu, WJ,Jiang, SS,Sun, JR,Chen, JL. 2017

[6]A Highly Expressed and Conserved Odorant Receptor for Identifying the Alarm Pheromone (E)-2-Decenal in Halyomorpha halys. Cao, Qian,Sun, Dongdong,Liu, Yang,Yang, Shiyong. 2025

[7]Molecular characterization and expression profiles of olfactory receptor genes in the parasitic wasp, Microplitis mediator (Hymenoptera: Braconidae). Gu, Shao-Hua,Wang, Shan-Ning,Guo, Yu-Yuan,Zhang, Yong-Jun,Ma, Long,Liu, Ze-Wen,Zhou, Jing-Jiang. 2014

[8]An olfactory receptor from Apolygus lucorum (Meyer-Dur) mainly tuned to volatiles from flowering host plants. Zhang, Jin,Li, Guo-Qing,Yan, Shu-Wei,Zhang, Jin,Liu, Yang,Wang, Gui-Rong.

[9]Narrow tuning of an odorant receptor to plant volatiles in Spodoptera exigua (Hubner). Liu, Yang,Guo, Mengbo,Cao, Depan,Wang, Guirong,Liu, Chengcheng,Guo, Mengbo,Dong, Shuanglin. 2014

[10]A chromosome-level genome assembly of the beet armyworm Spodoptera exigua. Bin Zhang,Bo Liu,Cong Huang,Longsheng Xing,Zaiyuan Li,Conghui Liu,Hongxu Zhou,Guiling Zheng,Jie Li,Jiachen Han,Qianlong Yu,Chunhong Yang,Wanqiang Qian,Fanghao Wan,Changyou Li. 2023

[11]Identification and Functional Characterization of an Odorant Receptor Expressed in the Genitalia of Helicoverpa armigera. Weihao Liu,Dongdong Sun,Xiaoqing Wang,Zhiqiang Wang,Yang Liu. 2024

[12]Molecular Characterization, Evolution and Expression Analysis of Ammonium Transporter from Four Closely Related Bactrocera Species (Tephritidae). Jie Zhang,Qi Wang,Chenhao Liu,Jiaying Liu,Qian Qian,Chuanjian Ru,Leyuan Liu,Shanchun Yan,Wei Liu,Guirong Wang. 2024

[13]Methyl eugenol regulates mating behavior in oriental fruit flies by enhancing lek attractiveness. Zhang, Jie,Liu, Wei,Chang, Hetan,Wang, Qi,Yuan, Jinxi,Liu, Leyuan,Liu, Chenhao,Zhang, Yan,Ru, Chuanjian,Yan, Shanchun,Hansson, Bill S.,Wang, Guirong. 2025

[14]褐飞虱延伸因子Elongationfactors-la的序列克隆和功能分析. 王渭霞,李凯龙,陈龙飞,陈洋,傅强. 2014

[15]RNAi和取食不同品种水稻后白背飞虱多铜氧化酶4基因的表达差异及生物学效应. 贾浩康,刘玉娣,侯茂林. 2019

[16]利用RNAi研究棉花SSH文库上调表达EST的功能. 尹国,路正营,李世云,韩永亮,张朝军,李付广. 2018

[17]褐飞虱甲硫氨酸合成酶基因NlylsMS的克隆及功能分析. 袁三跃,唐耀华,万品俊,李国清,傅强. 2015

[18]东北梅花鹿Galectin-1基因RNAi载体的构建. 秦欣欣,孙红梅,褚文辉,王大涛,郭倩倩,章秀婷,李春义. 2013

[19]RNA干扰技术在昆虫中的应用. 曹雅忠,尹姣,杜光青,李克斌,樊东. 2013

[20]梅花鹿ColX基因的RNAi重组慢病毒载体的构建及鉴定. 褚文辉,赵海平,杨福合,邢秀梅,李春义. 2010

作者其他论文 更多>>