数字农科院2.0

Self-Assembled Nanoparticle Vaccines Elicit Robust Protective Immune Responses against Type O Foot-and-Mouth Disease Virus Infection

文献类型： 外文期刊

作者： Cao, Nan;Li, Yamei;Zhao, Qiongqiong;Yao, Manman;Ren, Xujiao;Tian, Linxing;Hu, Zihui;Diao, Feifei;Li, Hehai;Lu, Zengjun;Rao, Guibo;Zhang, Huawei;Li, Kun;Cao, Sheng;Li, Xiangmin;Qian, Ping

作者机构：

关键词： foot-and-mouth disease;epitopes;nanoparticlevaccines;prokaryotic system expression;protectiveresponse

期刊名称： ACS NANO

ISSN： 1936-0851

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Foot-and-mouth disease virus (FMDV), a critical pathogen in the global livestock industry, has long been a focal point of international disease control strategies. This study developed a nanoparticle-based FMDV vaccine platform. We fused the FMDV immunodominant epitope (VP1-G-H-loop) and T-cell epitope (T3D) with the nanoparticle scaffold lumazine synthase (LS), efficiently producing the T3D-LS-LOOP nanoparticle vaccine using the prokaryotic expression system Escherichia coli (BL21). Mouse experiments showed that the T3D-LS-LOOP nanoparticle vaccine induced a more robust immune response than the LS-LOOP nanoparticle vaccine. To evaluate the role of different scaffolds in FMDV antigen presentation, we substituted the LS scaffold with Ferritin (Ft) and beta-annulus peptide (beta) nanoparticles, generating T3D-beta-LOOP and T3D-Ft-LOOP nanoparticle proteins. The results demonstrated that the LS scaffold-mediated T3D-LS-LOOP nanoparticle vaccine induced the highest neutralizing antibody (NAb) titers (1:28.5) and an effective T-cell immune response, providing 100% protection in mice, outperforming T3D-Ft-LOOP (66.7%) and T3D-beta-LOOP (83.3%) nanoparticle vaccines. Encouragingly, the T3D-LS-LOOP nanoparticle vaccine also induced high NAb titers against different lineages (Mya98/Cathay/PanAsia) of type O FMDV and a strong Th2-biased cellular immune response in swine, with no adverse reactions observed postvaccination, achieving complete protection against a type O FMDV challenge in swine.

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