数字农科院2.0

Remapping the spatial distribution of neutralizing sites and their immunodominance on the capsid of different topotypes of FMDV serotype O by site-directed competitive ELISA for detection of neutralizing antibodies

文献类型： 外文期刊

作者： Zhao, Qiongqiong;Li, Fengjuan;Huang, Shulun;Xing, Xiangchuan;Sun, Ying;Li, Pinghua;Bao, Huifang;Fu, Yuanfang;Sun, Pu;Bai, Xingwen;Yuan, Hong;Ma, Xueqing;Zhao, Zhixun;Zhang, Jing;Wang, Jian;Wang, Tao;Li, Dong;Zhang, Qiang;Qian, Ping;Cao, Yimei;Li, Kun;Li, Xiangmin;Lu, Zengjun

作者机构：

关键词： foot-and-mouth disease virus;serotype O;neutralizing antigenic sites;neutralizing antibody;competitive ELISA

期刊名称： MICROBIOLOGY SPECTRUM

ISSN：

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： The foot-and-mouth disease virus (FMDV) serotype O contains at least five neutralizing antigenic sites, yet the structural relationship and antibody abundance remain poorly characterized. This study identifies six distinct neutralizing antigenic sites by evaluating 27 host-derived neutralizing antibodies (NAbs) using competitive enzyme-linked immunosorbent assay (cELISA). These sites include the VP1 G-H loop, VP1 C-terminus, site 2, site 4, site 6, and site 7. Notably, classical sites 1 and 5 were reclassified into the VP1 G-H loop and VP1 C-terminus classes. Sites 2 and 4 align with classical classifications, targeting independent epitopes on VP2 and VP3, respectively. We identified two novel sites: site 6, which involves extensive interactions with the G-H loop, C-terminus of VP1 and VP3, and site 7, which interacts with both VP2 and VP3. Sera from cattle, sheep, and pigs immunized with four serotype O lineages (O/SCGH/2016, O/Mya/98, O/Tibet/99, and O/XJ/2017) were used to evaluate the immunodominance of these sites. NAb responses favored site 4 for O/SCGH/2016 and the VP1 G-H loop for O/XJ/2017. Immunization effectiveness varied by strains and host species: O/XJ/2017 and O/Tibet/99 were effective in sheep, while O/Mya/98 showed reduced efficacy; O/Tibet/99 showed good immunogenicity in pigs. No significant differences were observed in cattle. There is a strong correlation (r = 0.8693) between NAb levels at site 6 and virus neutralization tests, suggesting its potential for use in alternative testing methods. This study describes the spatial distribution of neutralizing sites and highlights strain-specific immunodominant epitopes and differential antibody responses across species, providing valuable insights for FMD prevention and control.IMPORTANCEThe antigenic structure of the foot-and-mouth disease virus (FMDV) serotype O is complex, and the immunodominant epitopes among different lineages remain poorly understood. This study classified the capsid surface epitopes into six distinct antigenic sites utilizing 27 neutralizing antibodies (NAbs) by paired competitive ELISAs (cELISAs). High-affinity NAbs were selected for site-directed cELISAs to assess antibody abundance in serum from cattle, sheep, and pigs vaccinated with various inactivated FMDV serotype O vaccines. Additionally, liquid-phase blocking ELISA (LPBE) and virus neutralization test (VNT) were employed to measure total antibody and NAb titers. Results indicated that immunodominant sites vary among different strains, with each strain exhibiting different immunogenicity across the three animal species. Notably, antibody titers from NAb pO18-10, targeting site 6 on VP1 and VP3, correlated strongly with VNT results. This study provides comprehensive insights into the antigenic structure of FMDV serotype O and lays the groundwork for developing new methods to detect NAbs.

分类号：

  • 相关文献

[1]Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease virus serotype O. Cao, Yimei,Li, Fengjuan,Xing, Xiangchuan,Zhang, Huiyan,Zhao, Qiongqiong,Sun, Pu,Fu, Yuanfang,Li, Pinghua,Ma, Xueqing,Zhang, Jing,Zhao, Zhixun,Yuan, Hong,Wang, Jian,Wang, Tao,Bao, Huifang,Bai, Xingwen,Li, Dong,Zhang, Qiang,Li, Kun,Lu, Zengjun. 2025

[2]Development and Validation of a Competitive ELISA Based on Bovine Monoclonal Antibodies for the Detection of Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype A. Yimei Cao,Kun Li,Xiangchuan Xing,Guoqiang Zhu,Yuanfang Fu,Huifang Bao,Xingwen Bai,Pu Sun,Pinghua Li,Jing Zhang,Xueqing Ma,Jian Wang,Zhixun Zhao,Dong Li,Zaixin Liu,Zengjun Lu. 2022

[3]Two cross-protective antigen sites on foot-and-mouth disease virus serotype o structurally revealed by broadly neutralizing antibodies from cattle. Kun Li,Yong He,Li Wang,Pinghua Li,Sheng Wang,Pu Sun,Huifang Bao,Yimei Cao,Xuerong Liu,Guoqiang Zhu,Yali Song,Xingwen Bai,Xueqing Ma,Yuanfang Fu,Hong Yuan,Jing Zhang,Jian Wang,Yingli Chen,Dong Li,Zhiyong Lou,Zaixin Liu,Zengjun Lu. 2021

[4]Identification of the largest non-essential regions of the C-terminal portion in 3A protein of foot-and-mouth disease virus for replication in cell culture. . 2020

[5]Novel chimeric foot-and-mouth disease virus-like particles harboring serotype O VP1 protect guinea pigs against challenge. Li, Haitao,Li, Zhiyong,Xie, Yinli,Qin, Xiaodong,Qi, Xingcai,Sun, Peng,Bai, Xingwen,Zhang, Zhidong,Li, Haitao,Ma, Youji.

[6]Porcine epidemic diarrhea virus virus-like particles produced in insect cells induce specific immune responses in mice. Wang, Cuiling,Wang, Cuiling,Yan, Feihu,Zheng, Xuexing,Wang, Hualei,Jin, Hongli,Wang, Chong,Zhao, Yongkun,Feng, Na,Wang, Tiecheng,Gao, Yuwei,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Jin, Hongli,Wang, Chong,Zheng, Xuexing,Wang, Hualei,Zhao, Yongkun,Feng, Na,Wang, Tiecheng,Gao, Yuwei,Yang, Songtao,Xia, Xianzhu.

[7]Highly pathogenic porcine reproductive and respiratory syndrome virus GP5 B antigenic region is not a neutralizing antigenic region. Leng, Chao-Liang,An, Tong-Qing,Chen, Jia-Zeng,Gong, Da-Qing,Peng, Jin-Mei,Yang, Yong-Qian,Wu, Jiang,Guo, Juan-Juan,Li, Deng-Yun,Zhang, Yi,Meng, Zhen-Xiang,Wu, Yu-Quan,Tian, Zhi-Jun,Tong, Guang-Zhi,Tong, Guang-Zhi.

[8]Evidence of VP1 of duck hepatitis A type 1 virus as a target of neutralizing antibodies and involving receptor-binding activity. Li, Xiaojun,Zhao, Ran,Li, Chenxi,Zhang, Tingting,Meng, Fanyi,Liu, Ming,Zhang, Yun,Lin, Wei.

[9]A spike-specific whole-porcine antibody isolated from a porcine B cell that neutralizes both genogroup 1 and 2 PEDV strains. Fu, Fang,Li, Lin,Shan, Lingling,Yang, Beibei,Shi, Hongyan,Zhang, Jiaoer,Feng, Li,Liu, Pinghuang,Wang, Hongfeng.

[10]Newcastle disease virus-vectored West Nile fever vaccine is immunogenic in mammals and poultry. Wang, Jinliang,Yang, Jie,Ge, Jinying,Hua, Ronghong,Liu, Renqiang,Wang, Xijun,Shao, Yu,Sun, Encheng,Wu, Donglai,Wen, Zhiyuan,Bu, Zhigao,Li, Xiaofeng,Qin, Chengfeng. 2016

[11]A Novel Conserved Linear Neutralizing Epitope on the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein. Rong Hong Hua,Shu Jian Zhang,Bei Niu,Jin Ying Ge,Ting Lan,Zhi Gao Bu. 2023

[12]Mapping of foot-and-mouth disease virus antigenic sites recognized by single-domain antibodies reveals different 146S particle specific sites and particle flexibility. Harmsen M.M.,Li H.,Sun S.,van der Poel W.H.M.,Dekker A.. 2023

[13]A method for screening CDV microneutralization activity in microvolume samples. Deng X.,Su J.,Hu B.,Bai X.. 2024

[14]A ferritin-based nanoparticle displaying a neutralizing epitope for foot-and-mouth disease virus (FMDV) confers partial protection in guinea pigs. Bingzhou Lu,Yi Ru,Rongzeng Hao,Yang Yang,Huanan Liu,Yajun Li,Yue Zhang,Yuhan Mao,Rui Yang,Yangyang Pan,Sijiu Yu,Haixue Zheng,Yan Cui. 2024

[15]Safety and immunogenicity of a SARS-CoV-2 mRNA vaccine (SYS6006) in minks, cats, blue foxes, and raccoon dogs. Hong Huo,Jinming Wang,Chan Li,Shuang Xiao,Han Wang,Jinying Ge,Gongxun Zhong,Zhiyuan Wen,Chong Wang,Qiaoling Lang,Lili Chen,Zilong Wang,Jinliang Wang,Xijun Wang,Xijun He,Yuntao Guan,Lei Shuai,Zhigao Bu. 2024

[16]Structural basis of different neutralization capabilities of monoclonal antibodies against H7N9 virus. Zhao, Bingbing,Sun, Zhenzhao,Wang, Shida,Shi, Zhibin,Jiang, Yongping,Wang, Xiurong,Deng, Guohua,Jiao, Peirong,Chen, Hualan,Wang, Jingfei. 2024

[17]Isolation and characterization of GI-19/L1148-like infectious bronchitis virus in China. Huixin Li,Jiabei Han,Bing Wang,Zongxi Han,Shengwang Liu. 2025

[18]A neutralizing nanobody-based liquid-phase blocking ELISA to assess the protective potency of Senecavirus A vaccine. Mu, Suyu,Pan, Songjia,Dong, Hu,Wu, Jinen,Zhang, Yun,Yin, Shuanghui,Wang, Jianing,Wei, Tian,Wen, Xiaobo,Guo, Huichen,Sun, Shiqi. 2025

[19]Establishment of a pseudovirus neutralization assay for TGEV. Haojie Wang,Jianxing Chen,Lihong Xue,Yue Sun,Tongqing An,Yue Wang,Hongyan Chen,Changqing Yu,Changyou Xia,He Zhang. 2025

[20]Development of a competitive ELISA method based on VLPs detecting the antibodies of serotype A FMDV. Yun Zhang,Rui Wang,Manyuan Bai,Xuefei Wang,Hu Dong,Jiajun Li,Suyu Mu,Haisheng Miao,Jianling Song,Shiqi Sun,Huichen Guo. 2022

作者其他论文 更多>>