数字农科院2.0

Transcriptome and Small-RNA Sequencing Reveals the Response Mechanism of Brassica napus to Waterlogging Stress

文献类型： 外文期刊

作者： Xianshuai Song;Lan Ge;Kaifeng Wang;Nian Wang;Xinfa Wang

作者机构：

关键词： Brassica napus;comparative transcriptome;microRNA;waterlogging

期刊名称： Plants

ISSN： 2223-7747

年卷期： 2025 年 14 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： Rapeseed (Brassica napus) is highly susceptible to waterlogging during the seedling stage; however, most of the studies on its gene expression under waterlogging stress have focused on transcriptional regulation, with little work conducted on post-transcriptional regulation to date. To elucidate this regulatory network, comparative transcriptome and miRNA analyses in the leaves and roots of rapeseed Zhongshuang11 (ZS11) were performed. Differentially expressed genes (DEGs) and miRNAs (DEmiRNAs) were identified by comparing the normal planting condition (the control group, CKT) with waterlogging treatment (WLT). DEGs identified in leaves and roots were enriched in different metabolic pathways, indicating their distinct mechanisms in response to waterlogging stress. In total, 68 and 82 DEmiRNAs were identified in leaves and roots, respectively, predicted to target 543 and 2122 DEGs in each tissue. Among these, 12 and 9 transcription factors (TFs) were exclusively targeted by DEmiRNAs in leaves and roots, respectively. Notably, six upregulated TFs in leaves were associated with the ethylene response and were predicted targets of bna-miR172 family members, and four TFs in roots participated in the ethylene response pathway. Furthermore, bna-miR169, along with novel-miR-23108 and novel-miR-42624 family members, played crucial roles in waterlogging response of rapeseed. Combining with the determination results of ethylene and jasmonic acid content, a preliminary model of miRNA-mediated gene expression regulation in rapeseed response to waterlogging stress was developed. These findings advance our understanding of transcriptional regulation under waterlogging and lay a theoretical foundation for improving rapeseed waterlogging tolerance.

分类号：

  • 相关文献

[1]Effects of waterlogging at different growth stages on physiological characteristics and seed yield of winter rape (Brassica napus L). Zhou, WJ,Lin, XQ. 1995

[2]Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus). Zhu, Yingchun,Yuan, Gaopeng,Gao, Bowen,An, Guolin,Li, Weihua,Si, Wenjing,Sun, Dexi,Liu, Junpu. 2022

[3]Stress-induced higher vein density in the C-3-C-4 intermediate Moricandia suffruticosa under drought and heat stress. Zhu, Xiaoyi,Liu, Jun,Sun, Xingchao,Kuang, Chen,Liu, Hongfang,Zhang, Liang,Zheng, Qiwei,Liu, Jing,Li, Jun,Wang, Hanzhong,Hua, Wei. 2022

[4]Comparative Transcriptome Analysis Reveals the Mechanisms Underlying Differences in Salt Tolerance Between indica and japonica Rice at Seedling Stage. Weilong Kong,Tong Sun,Chenhao Zhang,Xiaoxiao Deng,Yangsheng Li. 2021

[5]Comparative Transcriptome Analyses of the Developmental Stages of Taenia multiceps. Wen Hui Li,Yang Yang,Nian Zhang Zhang,Jian Kui Wang,Yin Ju Liu,Li Li,Hong Bin Yan,Wan Zhong Jia,Baoquan Fu. 2021

[6]Comparative Transcriptome Analyses of the Developmental Stages of Taenia multiceps. Wen Hui Li,Yang Yang,Nian Zhang Zhang,Jian Kui Wang,Yin Ju Liu,Li Li,Hong Bin Yan,Wan Zhong Jia,Baoquan Fu. 2021

[7]Transcriptomic differences in response to Metarhizium anisopliae and Trichoderma harzianum uncovers major regulative genes and pathways for establishment of beneficial relationship in peanut. Duozi Yan,Ni Cai,Xiangqun Nong,Guangjun Wang,Qinglei Wang,Hidayat Ullah,Xiongbing Tu,Zehua Zhang. 2022

[8]Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to Meloidogyne incognita. Yingchun Zhu,Gaopeng Yuan,Renzong Zhao,Guolin An,Weihua Li,Wenjing Si,Junpu Liu,Dexi Sun. 2022

[9]The Chromosome-Level Assembly of Ramie (Boehmeria Nivea L.) Genome Provides Insights into Molecular Regulation of Fiber Fineness. Kunmei Chen,Yao Ming,Mingbao Luan,Ping Chen,Junhui Chen,Heping Xiong,Jikang Chen,Bin Wu,Mingzhou Bai,Gang Gao,Qianqian Zhang,Xiaofei Wang,Jianbo Jian,Chunming Yu,Aiguo Zhu. 2023

[10]Oil candidate genes in seeds of cotton (Gossypium hirsutum L.) and functional validation of GhPXN1. Chenxu Gao,Xiao Han,Zhenzhen Xu,Zhaoen Yang,Qingdi Yan,Yihao Zhang,Jikun Song,Hang Yu,Renju Liu,Lan Yang,Wei Hu,Jiaxiang Yang,Man Wu,Jisheng Liu,Zongming Xie,Jiwen Yu,Zhibin Zhang. 2023

[11]milR20 negatively regulates the development of fruit bodies in Pleurotus cornucopiae. Yuhui Qi,Chenyang Huang,Mengran Zhao,Xiangli Wu,Guangyu Li,Yingjie Zhang,Lijiao Zhang. 2023

[12]Comparative Transcriptome Reveals Conserved Gene Expression in Reproductive Organs in Solanaceae. Zhang, Lingkui,Chong, Yipeng,Yang, Xiaolong,Fan, Wenyuan,Cheng, Feng,Li, Ying,Hou, Xilin,Zhang, Kang. 2025

[13]Comparative transcriptome analysis provides a glance into the regulation of the Krüppel homolog 1 on the reproduction and diapause of the predatory ladybeetle, Coccinella septempunctata. Junjie Chen,Xiaoxiao Liu,Shunda Han,Maosen Zhang,Zhaohan Liu,Yuyan Li,Lisheng Zhang. 2025

[14]Effects of nitric oxide on some physiological characteristics of maize seedlings under waterlogging. Wang, Ben,Li, Cuihua,Zhu, Yong,Tian, Xiaohai,Zou, Huawen,Liu, Hongfang,Ma, Guohui. 2011

[15]Comparison of transcriptomes undergoing waterlogging at the seedling stage between tolerant and sensitive varieties of Brassica napus L.. Zou Xi-ling,Zeng Liu,Lu Guang-yuan,Cheng yong,Xu Jin-song,Zhang Xue-kun. 2015

[16]Simulating the impact of flooding on wheat yield - Case study in East China. Li, Sanai,Tompkins, A. M.,Li, Sanai,Lin, Erda,Ju, Hui.

[17]Effects of waterlogging on nitrogen accumulation and alleviation of waterlogging damage by application of nitrogen fertilizer and mixtalol in winter rape (Brassica napus L). Zhou, W,Zhao, D,Lin, X. 1997

[18]Morpho-anatomical and physiological responses to waterlogging of sesame (Sesamum indicum L.). Wei, Wenliang,Li, Donghua,Wang, Linhai,Ding, Xia,Zhang, Yanxin,Gao, Yuan,Zhang, Xiurong. 2013

[19]The Transcriptome of Brassica napus L. Roots under Waterlogging at the Seedling Stage. Zou, Xiling,Tan, Xiaoyu,Hu, Chengwei,Zeng, Liu,Lu, Guangyuan,Fu, Guiping,Cheng, Yong,Zhang, Xuekun. 2013

[20]Screening and Identification of Waterlogging Tolerant Rapeseed (Brassica napus L.) During Germination Stage. Zou, Xi-ling,Cong, Ye,Cheng, Yong,Lu, Guang-yuan,Zhang, Xue-kun. 2013

作者其他论文 更多>>