数字农科院2.0

An ultrasensitive lateral flow immunoassay platform for foodborne biotoxins and pathogenic bacteria based on carbon-dots embedded mesoporous silicon nanoparticles fluorescent reporter probes

文献类型： 外文期刊

作者： Wang Y.; Deng C.; Qian S.; Li H.; Fu P.; Zhou H.; Zheng J.

作者机构：

关键词： Bacteria; Carbon; Fluorescence; Food microbiology; Immunology; Mesoporous materials; Nanoparticles; Particle size; Silicon; Carbon dots; Detection limits; Fluorescence probes; Foodborne pollutant; Lateral flow immunoassay; Mesoporous; Mesoporous silicon nanoparticle; Silicon nanoparticles; Staphylococcus aureus; Test strips; Probes; aflatoxin B1; carbon; fluorescent dye; mesoporous silica nanoparticle; nanobead; silane; aflatoxin B1; carbon; fluorescent dye; nanoparticle; silicon; absorption spectroscopy; Article; chemical modification; controlled study; covalent bond; fluorescence; fluorescence intensity; food; foodborne transmission; lateral flow immunochromatography; limit of detection; nonhuman; particle size; quantitative analysis; quantum yield; spectrofluorometry; Staphylococcus aureus; transmission electron microscopy; ultraviolet visible spectroscopy; zeta potential; immunoassay; pollutant; procedures; Staphylococcus aureus; Aflatoxin B1; Carbon; Environmental Pollutants; Fluo

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2023 年 399 卷

页码：

收录情况： SCIE（2023版） ; ; EI（2023版）

摘要： Lateral flow immunoassays (LFIAs) are routine methods for rapid foodborne pollutants screening, with detection limits that are closely associated with the label probes used. The exploitation of high performance and robust probe is highly desirable, and remains a great challenge. Herein, we reported an emerging fluorescent nanobeads i.e. carbon-dots (CD) covalently incorporated mesoporous silicon nanoparticles (CD-MSNs) for LFIAs. CD-MSNs revealed brighter fluorescence, larger particle size and more modification sites in comparison with those of single CD. After bio-functionalisation, CD-MSNs probes were introduced to construct LFIA test strips, and designed for ultrasensitive detection of aflatoxin B1 (AFB1) and Staphylococcus aureus (S. aureus), two representative foodborne pollutants, based on the competitive and sandwich models, respectively. Very competitive quantitative detection limits i.e. 0.05 ng/mL and 102 cfu/mL were correspondingly obtained. Additionally, the test strips were successfully applied to rapidly and accurately screen AFB1 and S. aureus in food samples, highlighting their practicality. © 2022 Elsevier Ltd

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